The enhanced effect of lupeol on the destruction of gastric cancer cells by NK cells

Wu, Xiaoting; Liu, Junquan; Lu, Xiaoting; Chen, Fuxing; Zhou, Zhonghai; Wang, Tao; Zhu, Sheng-Ping; Fei, Sujuan

doi:10.1016/j.intimp.2013.04.017

Cited by 50 publications

(31 citation statements)

References 41 publications

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“…The above results are in accordance to the report that the lupeol treatment permeabilizes the cell wall allowing the leakage of LDH in gastric cancer cells leading to cell death. [19] The nature of the compound to induce cell death in the MCF-7 cells was proved with the results of AO/EtBr staining [Figure 4]. The control untreated live cells exhibited green cells specifying the absence of cell death and the compound treated cells were found to be stained as red cells signifying the induction of cell death by the compound.…”

Section: Resultsmentioning

confidence: 99%

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In vitro evaluation of anticancer potentials of lupeol isolated from Elephantopus scaber L. on MCF-7 cell line

Pitchai¹,

Roy²,

Ignatius³

2014

J Adv Pharm Technol Res

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Lupeol is a triterpenoid, present in most of the medicinally effective plants and possess a wide range of biological activity against human diseases. The present study aims at evaluating the anticancer potentials of lupeol, isolated from the leaves of Elephantopus scaber L. and thereby explores its action on key cancer marker, Bcl-2. The effect of lupeol on the cell viability of MCF-7 was determined by MTT and lactate dehydrogenase assays at different concentrations. The efficacy of the compound to induce cell death was analyzed using AO/EtBr staining. Phase contrast microscopic analysis provided the changes in cell morphology of the compound treated normal breast cells (MCF-10A) and MCF-7 cells. The expression of Bcl-2 and Bcl-xL proteins in the normal, cancer and lupeol treated cancer cell was analyzed by western blotting. Lupeol induced an effective change in the cell viability of MCF-7 cells with IC50 concentration as 80 μM. Induction of cell death, change in cell morphology and population of the cancer cells was observed in the lupeol treated cells, but the normal cells were not affected. The compound effectively downregulated Bcl-2 and Bcl-xL protein expressions, which directly contribute for the induction of MCF-7 cell apoptosis. Conclusion: Thus, lupeol acts as an anticancer agent against MCF-7 cells and is a potent phytodrug to be explored further for its cytotoxic mechanism.

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Section: Resultsmentioning

confidence: 99%

“…[13] It is also evident that the compound is a potential agent against other cancers such as human prostate, colorectal, skin, liver and gastric cancer. [141516171819]…”

Section: Introductionmentioning

confidence: 99%

In vitro evaluation of anticancer potentials of lupeol isolated from Elephantopus scaber L. on MCF-7 cell line

Pitchai¹,

Roy²,

Ignatius³

2014

J Adv Pharm Technol Res

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“…Liu et al 101 isolated eight compounds including 5 sterols from L. betulina, while Fakoya & Oloketuyi 9 , reported phenolic and steroids compounds are present in EE of this mushroom. Lupeol, one terpenoid, is also present in LBE, library search exhibited that it facilitated significant inhibition of growth of breast cancer cell line 102 , prostate 103 , colorectal 104 , and gastric cancer 105 . GCMS profile of LBE also showed the presence of 4'-Hydroxy-6-methoxyaurone and this compound inhibits cancer cells to acquire drug resistant and also cytotoxicity against mouse RAW264.7 cell 106 .…”

Section: Discussionmentioning

confidence: 99%

Anti-cancer property of Lenzites betulina (L) Fr. on cervical cancer cell lines and its anti-tumor effect on HeLa-implanted mice

Sanyal

Ghosh

2019

Preprint

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In global scenario cervical cancer is increasing. New drugs from natural compounds are in search. Mushrooms are now recognized as miniature pharmaceutical factories producing hundreds of novel constituents. We have taken ethanolic extract Lenzities betulina (LBE) wild mushroom for evaluation of its as anti-cancer property against cervical cancer cell lines e.g. HeLa, CaSki and SiHa and anti tumor activity against HeLa implanted tumor on mice. The extraction was done by dip and stirring method in 90% ethanol for 72 h. For evaluation of anti-cervical cancer, several assays were performed such as MTT assay, cell morphology by phase contrast microscope and F-action polymerization by Laser scanning confocal microscope and nuclear morphology DAPI staining under inverted fluorescence microscope, MMP, ROS, cell cycle, autophagy and stem cell population by flow cytometry and DNA laddering were done. Western blotting was done for protein expression. To evaluate anti-metastatic activity, anti-cologenic assay and wound healing assay were adopted. For chemo-analysis of the LBE, GC-MS was done. The results from Cytotoxicity assay showed that at highest dose of LBE (1000 µg/ml) after 24 h, percentage of cell inhibitions were 85.13 %, 77.13 % and 47.70 % against HeLa , CaSki and SiHa respectively and the calculated IC 50 values were 492.52 ± 2.6 µg/ml, 612.22 ± 4.2 µg/ml, and 1210.30 ± 6.4 µg/ml respectively . Depending upon the cytotoxicity screening, HeLa cell line was considered for the further studies. Cell morphology study exhibited that LBE treated HeLa cells became round from normal spindle shape. DAPI staining showed that LBE treated nucleus became condensed and fragmented. DNA fragmentation at 230 and 300 base pair zone from agarose gel assay was observed. LBE induced ROS generation and reduced MMP. It up regulated the expression of apoptotic genes and p53 while down regulated Bcl2, pro-caspase 3 and pro caspase-9 gene. Cell cycle was arrested at G2/M checkpoint. Autophagic induction was exhibited by vacuole formation in treated cells. CSC population of treated cells was reduced and F-actin polymerization was observed in treated cells. In addition, LBE suppressed metastatic nature by inhibition of cell migration and colonization. The inhibition of growth of the tumors in HeLa cell-implanted mice showed that treatment with 50 mg LBE/kg of body weight of mice led to a marked reduction in the volume (93.22 ± 9.2 %) and weight (90.42 ±9.55 %) of the tumors. The GC-MS profile of LBE shows that out of 69 compounds, 9, 12-Octadecadienoic acid (Z, Z) and 8, (3.beta22E) are in a significantly higher proportion with the percentage peak area 22.13 and 19.72 respectively. Library search for bioactivity showed that these compounds are anti-cancerous and interestingly 4'-Hydroxy-6methoxyaurone binding with P-glycoprotein inhibits the cancer cells to become drug resistant. In conclusion, LBE is very prominent anti-cervical cancer having a lot of anti-cancerous compounds which are probably acting synergistically. This report of anti-ce...

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“…cervical, head and neck, lung, and prostate cancer (Prasad et al, 2008;Bhattacharyya et al, 2017;Min et al, 2019) sensitizing chemotherapy ABCG2, MAPK1, EIF2A, CASP3 colon cancer (Chen et al, 2018b) enhancing immunity AKT1, BCL2, CTNNB1, IFNG, LAMP1, MAPK2/3, PIK3CA, PRF1 gastric cancer (Wu et al, 2013) A B This study systematically explored the anti-cancer mechanisms of Litchi using network pharmacology methods. However, it was distinct from traditional network pharmacology research, in which, the components and targets of a natural herb were mainly predicted based on online databases, followed by experimental verification in vitro and in vivo.…”

Section: Phenolic Acidsmentioning

confidence: 99%

Mapping Pharmacological Network of Multi-Targeting Litchi Ingredients in Cancer Therapeutics

Cao

Han

et al. 2020

Front. Pharmacol.

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Considerable pharmacological studies have demonstrated that the extracts and ingredients from different parts (seeds, peels, pulps, and flowers) of Litchi exhibited anticancer effects by affecting the proliferation, apoptosis, autophagy, metastasis, chemotherapy and radiotherapy sensitivity, stemness, metabolism, angiogenesis, and immunity via multiple targeting. However, there is no systematical analysis on the interaction network of "multiple ingredients-multiple targets-multiple pathways" anticancer effects of Litchi. In this study, we summarized the confirmed anticancer ingredients and molecular targets of Litchi based on published articles and applied network pharmacology approach to explore the complex mechanisms underlying these effects from a perspective of system biology. The top ingredients, top targets, and top pathways of each anticancer function were identified using network pharmacology approach. Further intersecting analyses showed that Epigallocatechin gallate (EGCG), Gallic acid, Kaempferol, Luteolin, and Betulinic acid were the top ingredients which might be the key ingredients exerting anticancer function of Litchi, while BAX, BCL2, CASP3, and AKT1 were the top targets which might be the main targets underling the anticancer mechanisms of these top ingredients. These results provided references for further understanding and exploration of Litchi as therapeutics in cancer as well as the application of "Component Formula" based on Litchi's effective ingredients.

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The enhanced effect of lupeol on the destruction of gastric cancer cells by NK cells

Cited by 50 publications

References 41 publications

In vitro evaluation of anticancer potentials of lupeol isolated from Elephantopus scaber L. on MCF-7 cell line

In vitro evaluation of anticancer potentials of lupeol isolated from Elephantopus scaber L. on MCF-7 cell line

Anti-cancer property of Lenzites betulina (L) Fr. on cervical cancer cell lines and its anti-tumor effect on HeLa-implanted mice

Mapping Pharmacological Network of Multi-Targeting Litchi Ingredients in Cancer Therapeutics

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