Mapping Pharmacological Network of Multi-Targeting Litchi Ingredients in Cancer Therapeutics

Cao, Sisi; Han, Yulan; Li, Qiaofeng; Chen, Yanjiang; Zhu, Dong; Su, Zhiheng; Guo, Hongwei

doi:10.3389/fphar.2020.00451

Cited by 14 publications

(6 citation statements)

References 245 publications

(189 reference statements)

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“…However, the seed, leaf, and skin of litchi were usually abandoned. Research studies have showed that they are rich in polyphenols, flavonoids, and saponins, which are demonstrated to have great medicinal value. − As in our previous report, there are a range of phenolic compounds, such as protocatechuic acid, protocatechuic aldehyde, procyanidin D, (−)-epicatechin, cinnamtannin B1, procyanidin A1, rutin, and phlorizin in litchi seeds . Pharmacological studies showed that litchi seeds had multiple bioactivities such as antiobesity, antioxidant, anti-inflammation, antihyperlipidemia, antidiabetes, hypoglycemic, and immunoregulation .…”

Section: Introductionmentioning

confidence: 80%

Polyphenol-Rich Extract from Litchi chinensis Seeds Alleviates Hypertension-Induced Renal Damage in Rats

Yao¹,

Liu²,

Yin³

et al. 2021

J. Agric. Food Chem.

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Litchi chinensis seed is a valuable byproduct of the subtropical fruit litchi (L. chinensis Sonn.), whose extract (LSE) has been confirmed to ameliorate dyslipidemia, hyperglycemia, and oxidative stress caused by type 2 diabetes. However, if LSE exerts an effect on anti-hypertension and hypertensive renal damage remains unknown. In this study, 13 polyphenols and one fatty acid were identified by UPLC-Q/TOF-MS. Network pharmacological analysis revealed that the therapeutic effects of LSE may be involved in multitargets and multipathways, such as the TNF signaling pathway, interleukin (IL)-6-mediated signaling pathway, NF-kappa B signaling pathway, removal of superoxide radicals, negative regulation of blood pressure, and so forth. Moreover, spontaneously hypertensive rats (SHRs) were daily gavaged with LSE (60 mg/kg) for 10 weeks. LSE remarkably reduced systolic blood pressure (SBP). The hypertension-induced renal damage was improved by suppressing inflammation and oxidative stress, which was consistent with the prediction of network pharmacology. In addition, LSE treatment remarkably increased the relative abundances of Lactobacillus and the production of short-chain fatty acids in the intestine. Our study indicated that a byproduct of litchi, namely, litchi seed, may be effective in reducing SBP and alleviating hypertensive renal damage.

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Section: Introductionmentioning

confidence: 80%

Polyphenol-Rich Extract from Litchi chinensis Seeds Alleviates Hypertension-Induced Renal Damage in Rats

Yao¹,

Liu²,

Yin³

et al. 2021

J. Agric. Food Chem.

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“…Litchi seeds have been traditionally used in Chinese herbal formulas for urologic neoplasms including prostate cancer, renal carcinoma and bladder cancer ( Hsu et al, 2012 ; Emanuele et al, 2018 ). In a previous study, we found that flavonoids were the most abundant chemical component among the extracts of Litchi seeds which exerted anti-PCa activity ( Guo et al, 2017 ; Cao et al, 2020 ). Other studies also found that flavonoids extracted from Litchi pericarp such as epicatechin and proanthocyanidin B2 had anticancer activities ( Koga et al, 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…Its seeds have been traditionally used in China for treating male urogenital diseases, including prostate cancer, prostatitis, benign prostatic hyperplasia and orchitis etc. ( Cao et al, 2020 ). Accumulating pharmacological evidence suggested that the extracts of Litchi seeds have anticancer effects and can inhibit metastatic tumor growth ( Hsu et al, 2012 ; Emanuele et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Total Flavonoids of Litchi Seed Attenuate Prostate Cancer Progression Via Inhibiting AKT/mTOR and NF-kB Signaling Pathways

et al. 2021

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Litchi seeds have been traditionally used in Chinese herbal formula for urologic neoplasms including prostate cancer (PCa). However, the effective components of Litchi seeds and the mechanisms of their actions on PCa cell growth and metastasis remain unclear. In this study, we investigated the effects and molecular mechanisms of the Total Flavonoid of Litchi Seed (TFLS) in PCa PC3 and DU145 cell lines. We found that TFLS significantly inhibited the PCa cell proliferation, induced apoptosis, and prevented cell migration and invasion. Furthermore, we observed that TFLS upregulated the expression of epithelial biomarker E-cadherin and downregulated mesenchymal biomarker Vimentin. TFLS also increased the expression of cleaved-PRAP and Bax, and decreased the expression of Bcl-2 in both PC3 and DU145 cells. Besides, TFLS inhibited AKT signaling pathway by reducing the phosphorylation of AKT and activities of downstream signal transducers including mTOR, IκBα and NF-kB. Finally, TFLS treated mice exhibited a significant decrease in tumor size without toxicity in major organs in vivo. These results indicated that TFLS could suppress PCa cell growth in vivo and inhibit PCa cell proliferation and metastasis in vitro through induction of apoptosis and phenotypic reversal of EMT, which may be achieved by inhibiting the AKT/mTOR and NF-κB signaling pathways. Taken together, our data provide new insights into the role of TFLS as a novel potent anti-cancer agent for the treatment of PCa.

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“…As a new paradigm in drug studies, derived from systems biology and bioinformatics, network pharmacology can reveal the underlying molecular mechanisms of Chinese herb medicine, via the construction and visualization of “medicine–target–disease” interaction network ( 21 , 22 ). Network pharmacology can reflect the “multiple components–multiple targets–multiple pathways” of anticancer effects of Chinese herb medicine ( 23 ), which is potential to solve the same problems of the D. officinale methanolic extract.…”

Section: Introductionmentioning

confidence: 99%

Anti-colon Cancer Effects of Dendrobium officinale Kimura & Migo Revealed by Network Pharmacology Integrated With Molecular Docking and Metabolomics Studies

Tao

Wang

et al. 2022

Front. Med.

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ObjectiveThe present study aimed to investigate the potential mechanism of Dendrobium officinale (D. officinale) on colorectal cancer and the relevant targets in the pathway using a network pharmacological approach.Methods(1) We identified the major bioactive components of D. officinale by UPLC-ESI-MS/MS and established the in-house library by using the literature mining method. (2) Target prediction was performed by SwissADME and SwissTargetPrediction. (3) A protein–protein interaction (PPI) network and component–target–pathway network (C-T-P network) were constructed. (4) The GO pathways and the KEGG pathway enrichment analysis were carried out by the Metascape database. (5) Molecular docking was performed by AutoDock software. (6) A series of experimental assays including cell proliferation, cell invasion and migration, and TUNEL staining in CRC were performed in CRC cell lines (HT-29, Lovo, SW-620, and HCT-116) to confirm the inhibitory effects of D. officinale.Results(1) In total, 396 candidate active components of D. officinale were identified by UPLC-ESI-MS/MS and selected from the database. (2) From OMIM, GeneCards, DrugBank, and TTD databases, 1,666 gene symbols related to CRC were gathered, and (3) 34 overlapping gene symbols related to CRC and drugs were obtained. (4) These results suggested that the anti-CRC components of D. officinale were mainly apigenin, naringenin, caffeic acid, γ-linolenic acid, α-linolenic acid, cis-10-heptadecenoic acid, etc., and the core targets of action were mainly ESR1, EGFR, PTGS2, MMP9, MMP2, PPARG, etc. (5) The proliferation of muscle cells, the regulation of inflammatory response, the response of cells to organic cyclic compounds, and the apoptotic signaling pathway might serve as principal pathways for CRC treatment. (6) The reliability of some important active components and targets was further validated by molecular docking. The molecular docking analysis suggested an important role of apigenin, naringenin, PTGS2, and MMP9 in delivering the pharmacological activity of D. officinale against CRC. (7) These results of the evaluation experiment in vitro suggested that D. officinale had a strong inhibitory effect on CRC cell lines, and it exerted anti-CRC activity by activating CRC cell apoptosis and inhibiting CRC cell migration and invasion.ConclusionThis study may provide valuable insights into exploring the mechanism of action of D. officinale against CRC.

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Mapping Pharmacological Network of Multi-Targeting Litchi Ingredients in Cancer Therapeutics

Cited by 14 publications

References 245 publications

Polyphenol-Rich Extract from Litchi chinensis Seeds Alleviates Hypertension-Induced Renal Damage in Rats

Polyphenol-Rich Extract from Litchi chinensis Seeds Alleviates Hypertension-Induced Renal Damage in Rats

Total Flavonoids of Litchi Seed Attenuate Prostate Cancer Progression Via Inhibiting AKT/mTOR and NF-kB Signaling Pathways

Anti-colon Cancer Effects of Dendrobium officinale Kimura & Migo Revealed by Network Pharmacology Integrated With Molecular Docking and Metabolomics Studies

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