The Embryo MADS Domain Protein AGAMOUS-Like 15 Directly Regulates Expression of a Gene Encoding an Enzyme Involved in Gibberellin Metabolism

Wang, Huai; Caruso, Leonardo V.; Downie, A. Bruce; Perry, Sharyn E.

doi:10.1105/tpc.021261

Cited by 165 publications

(150 citation statements)

References 52 publications

(93 reference statements)

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“…The recovered transgenic soybean plants constitutively expressing AGL15 appear relatively normal, with some plants showing perhaps increased branching or darker green color combined with reduced seed set and some meristem abnormalities. All of these potential phenotypes would be consistent with phenotypes observed with ectopic expression of AGL15 in Arabidopsis (Fernandez et al, 2000;Wang et al, 2004). However, because the plants thus far are from tissue culture, it is premature to make any conclusions about effects of the transgene on plant morphology or development at this time.…”

Section: Discussionsupporting

confidence: 51%

“…Also intriguing are recent results that indicate that LEC2 may directly induce expression of AGL15 (Braybrook et al, 2006). Like LEC2 and FUS3, AGL15 impacts upon bioactive GA accumulation, but AGL15 mediates its effect at least in part by directly inducing expression of AtGA2ox6 (At1g02400) that encodes a GA 2-oxidase that catabolizes biologically active GA (Wang et al, 2004). Expression of this GA 2-oxidase affects somatic embryo development from the shoot apical meristem (SAM) of liquid culture grown seedlings in the presence of 2,4-D (Wang et al, 2004).…”

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confidence: 99%

“…Like LEC2 and FUS3, AGL15 impacts upon bioactive GA accumulation, but AGL15 mediates its effect at least in part by directly inducing expression of AtGA2ox6 (At1g02400) that encodes a GA 2-oxidase that catabolizes biologically active GA (Wang et al, 2004). Expression of this GA 2-oxidase affects somatic embryo development from the shoot apical meristem (SAM) of liquid culture grown seedlings in the presence of 2,4-D (Wang et al, 2004). We report here that loss-of-function alleles of AGL15, in some cases when combined with loss of function of AGL18, a closely related family member that is redundant to AGL15 in control of flowering time (Adamczyk et al, 2007), significantly impairs the ability to form somatic embryos in two systems in Arabidopsis.…”

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The MADS-Domain Transcriptional Regulator AGAMOUS-LIKE15 Promotes Somatic Embryo Development in Arabidopsis and Soybean

et al. 2008

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The MADS-domain transcriptional regulator AGAMOUS-LIKE15 (AGL15) has been reported to enhance somatic embryo development when constitutively expressed. Here we report that loss-of-function mutants of AGL15, alone or when combined with a loss-of-function mutant of a closely related family member, AGL18, show decreased ability to produce somatic embryos. If constitutive expression of orthologs of AGL15 is able to enhance somatic embryo development in other species, thereby facilitating recovery of transgenic plants, then AGL15 may provide a valuable tool for crop improvement. To test this idea in soybean (Glycine max), a full-length cDNA encoding a putative ortholog of AGL15 was isolated from soybean somatic embryos. Subsequently, the corresponding genomic region of the gene was obtained. This gene, designated GmAGL15, encodes a protein with highest similarity to AGL15 from Arabidopsis (Arabidopsis thaliana) and Brassica napus that accumulates to its highest amount in embryos in these species. Like Arabidopsis and Brassica AGL15, GmAGL15 was preferentially expressed in developing embryos. When ectopically overexpressed the soybean protein was able to enhance somatic embryo development in soybean.

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Section: Discussionsupporting

confidence: 51%

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confidence: 99%

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confidence: 99%

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The MADS-Domain Transcriptional Regulator AGAMOUS-LIKE15 Promotes Somatic Embryo Development in Arabidopsis and Soybean

et al. 2008

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“…ChIP was performed as described (Wang et al, 2004;Zheng et al, 2009;Zheng and Perry, 2011) except that a modified sonication buffer with lower sarkosyl was used (10 mM potassium phosphate, pH 7, 0.1 M NaCl, 0.25% sarkosyl, 10 mM EDTA, and 0.1 mM phenylmethylsulfonyl fluoride). The immunoprecipitation buffer from Zheng and Perry (2011) was also modified to lower ionic detergents (50 mM Tris, pH 7.5, 150 mM NaCl, 1% Triton X-100, and 1.2 mM EDTA, pH 8.0), and 4 volumes of immunoprecipitation buffer was added to 1 volume of solubilized chromatin in modified sonication buffer.…”

Section: Chip-chip and Data Analysismentioning

confidence: 99%

Identification of Direct Targets of FUSCA3, a Key Regulator of Arabidopsis Seed Development

2013

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FUSCA3 (FUS3) is a B3 domain transcription factor that is a member of the LEAFY COTYLEDON (LEC) group of genes. The LEC genes encode proteins that also include LEC2, a B3 domain factor related to FUS3, and LEC1, a CCAAT box-binding factor. LEC1, LEC2, and FUS3 are essential for plant embryo development. All three loss-of-function mutants in Arabidopsis (Arabidopsis thaliana) prematurely exit embryogenesis and enter seedling developmental programs. When ectopically expressed, these genes promote embryo programs in seedlings. We report on chromatin immunoprecipitation-tiling array experiments to globally map binding sites for FUS3 that, along with other published work to assess transcriptomes in response to FUS3, allow us to determine direct from indirect targets. Many transcription factors associated with embryogenesis are direct targets of FUS3, as are genes involved in the seed maturation program. FUS3 regulates genes encoding microRNAs that, in turn, control transcripts encoding transcription factors involved in developmental phase changes. Examination of direct targets of FUS3 reveals that FUS3 acts primarily or exclusively as a transcriptional activator. Regulation of microRNA-encoding genes is one mechanism by which FUS3 may repress indirect target genes. FUS3 also directly up-regulates VP1/ABI3-LIKE1 (VAL1), encoding a B3 domain protein that functions as a repressor of transcription. VAL1, along with VAL2 and VAL3, is involved in the transition from embryo to seedling development. Many genes are responsive to FUS3 and to VAL1/VAL2 but with opposite regulatory consequences. The emerging picture is one of complex cross talk and interactions among embryo transcription factors and their target genes.

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“…Moreover, overexpression of the Arabidopsis AGAMOUS-Like 15 (AGL15) gene and its homologous gene in soybean, which encode MADS domaincontaining proteins, enhances the formation of secondary embryos and somatic embryos [16,17]. The promotion of somatic embryo formation by AGL15 is attributed, at least in part, to a reduced GA level [18]. This notion is consistent with the observation made in the pkl mutant that displays a GA-deficiency phenotype [9,10], and the observation that FUS3, encoding a B3 transcription factor critical for embryogenesis, negatively regulates GA biosynthesis [19].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis

et al. 2008

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Formation of somatic embryos from non-germline cells is unique to higher plants and can be manipulated in a variety of species. Previous studies revealed that overexpression of several Arabidopsis genes, including WUSCHEL (WUS)/PLANT GROWTH ACTIVATOR6 (PGA6), BABY BOOM, LEAFY COTYLEDON1 (LEC1), and LEC2, is able to cause vegetative-to-embryonic transition or the formation of somatic embryos. Here, we report that a gain-offunction mutation in the Arabidopsis PGA37 gene, encoding the MYB118 transcription factor, induced vegetative-toembryonic transition, the formation of somatic embryos from root explants, and an elevated LEC1 expression level. Double mutant analysis showed that WUS was not required for induction of somatic embryos by PGA37/MYB118. In addition, overexpression of MYB115, a homolog of PGA37/MYB118, caused a pga37-like phenotype. A myb118 myb115 double mutant did not show apparent developmental abnormalities. Collectively, these results suggest that PGA37/ MYB118 and MYB115 promote vegetative-to-embryonic transition, through a signaling pathway independent of WUS.

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The Embryo MADS Domain Protein AGAMOUS-Like 15 Directly Regulates Expression of a Gene Encoding an Enzyme Involved in Gibberellin Metabolism

Cited by 165 publications

References 52 publications

The MADS-Domain Transcriptional Regulator AGAMOUS-LIKE15 Promotes Somatic Embryo Development in Arabidopsis and Soybean

The MADS-Domain Transcriptional Regulator AGAMOUS-LIKE15 Promotes Somatic Embryo Development in Arabidopsis and Soybean

Identification of Direct Targets of FUSCA3, a Key Regulator of Arabidopsis Seed Development

Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis

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