Identification of Direct Targets of FUSCA3, a Key Regulator of Arabidopsis Seed Development

Wang, Fangfang; Perry, Sharyn E.

doi:10.1104/pp.112.212282

Cited by 205 publications

(250 citation statements)

References 79 publications

(168 reference statements)

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“…In this model, and in addition to miR156, miRNA166 is also relevant since one of its target genes, PHB, is a direct activator of LEC2 (Tang et al 2012), revealing a regulatory module consisting of a miRNA, its target genes (PHB and PHV), and a direct target of PHB (LEC2). Additionally, FUS3 is involved in feedback regulation of the activation network through its direct targets AGL15, MYB118 and miRNA156 encoding gene (Wang and Perry 2013). By searching available resources and performing phylogenetic analyses a list of AFL ortologues in the plant kingdom has been produced and allowed to conclude that the core function of the AFL network is conserved among Spermatophyte (Devic and Roscoe 2016).…”

Section: Micrornas As Components Of Gene Regulatory Network In Seed mentioning

confidence: 99%

The pivotal role of small non-coding RNAs in the regulation of seed development

Rodrigues

Miguel

2017

Plant Cell Rep

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Section: Micrornas As Components Of Gene Regulatory Network In Seed mentioning

confidence: 99%

The pivotal role of small non-coding RNAs in the regulation of seed development

Rodrigues

Miguel

2017

Plant Cell Rep

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“…The VAL proteins have a B3 DNA-binding domain that is proposed to recognize RY elements (Suzuki et al, 2007). Since MIR156A and MIR156C contain RY motifs (Wang and Perry, 2013), we reasoned that they might be targets of the VAL proteins. To investigate this, we first analyzed the expression levels of the pri-MIR156s in val1/2 mutants and compared them with the levels in wild-type and strong atbmi1a/b seedlings at 10 DAG (Fig.…”

Section: The Levels Of H2aub and H3k27me3 Marks In Atbmi1mentioning

confidence: 99%

“…Among these genes, MIR156A and MIR156C were recently shown to be direct targets of the seed maturation gene FUS3. FUS3 activates MIR156A/C expression during seed development, and this expression is important after germination to delay the juvenile-to-adult vegetative phase transition (Wang and Perry, 2013). MIR156A and MIR156C contain RY elements at their 59 end and into/through the gene, which are DNA elements specifically recognized by the B3 DNA-binding domain of FUS3 (Wang and Perry, 2013).…”

Section: Atbmi1a/b Mutants Have An Extended Juvenile Phasementioning

confidence: 99%

“…On the other hand, recent evidence indicates that the seed maturation gene FUSCA3 (FUS3) contributes to the direct expression of the primary transcripts of MIR156A and MIR156C (pri-MIR156A and pri-MIR156C) in the developing seed and that this expression is important after germination to delay the juvenile-to-adult vegetative phase transition (Wang and Perry, 2013). However, upstream effectors mediating the age-dependent decline in miR156 levels are largely unknown.…”

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confidence: 99%

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Deciphering the Role of POLYCOMB REPRESSIVE COMPLEX1 Variants in Regulating the Acquisition of Flowering Competence in Arabidopsis

et al. 2015

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Polycomb group (PcG) proteins play important roles in regulating developmental phase transitions in plants; however, little is known about the role of the PcG machinery in regulating the transition from juvenile to adult phase. Here, we show that Arabidopsis (Arabidopsis thaliana) B lymphoma Moloney murine leukemia virus insertion region1 homolog (BMI1) POLYCOMB REPRESSIVE COMPLEX1 (PRC1) components participate in the repression of microRNA156 (miR156). Loss of AtBMI1 function leads to the up-regulation of the primary transcript of MIR156A and MIR156C at the time the levels of miR156 should decline, resulting in an extended juvenile phase and delayed flowering. Conversely, the PRC1 component EMBRYONIC FLOWER (EMF1) participates in the regulation of SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE and MIR172 genes. Accordingly, plants impaired in EMF1 function displayed misexpression of these genes early in development, which contributes to a CONSTANSindependent up-regulation of FLOWERING LOCUS T (FT) leading to the earliest flowering phenotype described in Arabidopsis. Our findings show how the different regulatory roles of two functional PRC1 variants coordinate the acquisition of flowering competence and help to reach the threshold of FT necessary to flower. Furthermore, we show how two central regulatory mechanisms, such as PcG and microRNA, assemble to achieve a developmental outcome.Polycomb group (PcG) proteins are conserved epigenetic regulators that mediate gene repression through the incorporation of histone-modifying marks (Calonje, 2014). As far as it is known, PcG proteins associate in two multiprotein complexes in Arabidopsis (Arabidopsis thaliana): POLYCOMB REPRES-SIVE COMPLEX1 (PRC1) and PRC2. The combined activity of the two complexes is required for stable repression of the target genes.The major function of PRC2 is to perform histone H3 lysine-27 trimethylation (H3K27me3) through the methyltransferase activity of CURLY LEAF (CLF) and SWINGER (SWN) during sporophyte development or of MEDEA in the endosperm (Chanvivattana et al., 2004). Other PRC2 components are the Drosophila melanogaster suppressor of zeste12 homologs VERNALIZATION2 (VRN2), EMBRYONIC FLOWER2 (EMF2), and FERTILIZATION-INDEPENDENT SEED2, which confer specificity to the resulting PRC2s even though they have some overlapping functions (Chanvivattana et al., 2004), and finally MULTICOPY SUPPRESSOR OF INHIBITORY REGULATOR OF THE RAS-CYCLIC AMP PATHWAY and FERTILIZATION-INDEPENDENT ENDOSPERM, which are common subunits for the different PRC2s (Derkacheva and Hennig, 2014). On the other hand, the identity of Arabidopsis PRC1 is not defined yet. PRC1-mediated function can be histone 2A monoubiquitination (H2Aub) dependent, through the E3 ubiquitin ligase activity of the PRC1 RING finger proteins Arabidopsis B lymphoma Moloney murine leukemia virus insertion region1 homolog 1A (AtBMI1A)/B/C and AtRING1A/B, or H2Aub independent, which requires the activity of the PRC1 component EMF1 (Bratzel et al., 2010(Bratzel et al., , 2012Yang et al., 2013a;Calo...

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“…17 Together with ABI3/ABI5/DELLA complex, FUS3 regulates the expression of heat-induced genes to promote thermoinhibition. 13,18,19 It will be important to determine if the accumulation of the ABI3/ABI5/DELLA complex at high temperature is also due to inhibition of proteasome activity by ABA. Given that GA partially rescues thermoinhibition and increases FUS3 degradation, it is expected that GA would have a stimulatory effect on proteasome activity and protein ubiquitination during germination, by antagonizing ABA action.…”

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confidence: 99%

Inhibition of FUSCA3 degradation at high temperature is dependent on ABA signaling and is regulated by the ABA/GA ratio

Chiu

Saleh

Gazzarrini

2016

Plant Signaling & Behavior

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During seed imbibition at supra-optimal temperature, an increase in the abscisic acid (ABA)/gibberellin (GA) ratio imposes secondary dormancy to prevent germination (thermoinhibition). FUSCA3 (FUS3), a positive regulator of seed dormancy, accumulates in seeds imbibed at high temperature and increases ABA levels to inhibit germination. Recently, we showed that ABA inhibits FUS3 degradation at high temperature, and that ABA and high temperature also inhibit the ubiquitin-proteasome system, by dampening both proteasome activity and protein polyubiquitination. Here, we investigated the role of ABA signaling components and the ABA antagonizing hormone, GA, in the regulation of FUS3 levels. We show that the ABA receptor mutant, pyl1-1, is less sensitive to ABA and thermoinhibition. In this mutant background, FUS3 degradation in vitro is faster. Similarly, GA alleviates thermoinhibition and also increases FUS3 degradation. These results indicate that inhibition of FUS3 degradation at high temperature is dependent on a high ABA/GA ratio and a functional ABA signaling pathway. Thus, FUS3 constitutes an important node in ABA-GA crosstalk during germination at supra-optimal temperature. KEYWORDS ABA; dormancy; FUSCA3; GA; germination; high temperature; protein degradation; proteasome; thermoinhibition; ubiquitin proteasome system; UPS Seed dormancy is an important adaptive trait that prevents seedling establishment under unfavourable conditions to maximize plant survival. Dormancy prevents pre-harvest sprouting of mature seeds and precocious germination or vivipary of immature seeds when still attached to the mother plant. These processes can lead to severe yield loss in agricultural crops. While primary dormancy is induced during seed maturation, secondary dormancy can be imposed in mature seeds with non-deep dormancy to prevent their germination under unfavourable growth conditions such as high temperature. [1][2] In Arabidopsis, the transcription factor FUSCA3 (FUS3) is essential for seed maturation and regulates several processes including the establishment of primary dormancy during embryogenesis. Loss-of-function fus3 embryos skip dormancy and can germinate precociously, while mutants ectopically expressing FUS3 post-embryonically display increased dormancy and show delayed germination, growth and flowering among several other phenotypes. 3-6 FUS3 represses developmental phase transitions by increasing the levels of the dormancy-promoting hormone, abscisic acid (ABA), while repressing the synthesis of the germination/growth-promoting hormone, gibberellin A (GA). 5,7,8 FUS3 is itself regulated by these hormones: the protein is more abundant in the presence of ABA and less abundant in the presence of GA. Exogenous GA application or reduction of endogenous ABA both partially rescue FUS3 overexpression phenotypes, such as the delayed growth, flowering and reduced cell cycling. 5 While FUS3 was shown to directly bind and repress GA biosynthetic genes, 4,8 the mechanisms behind the regulation of FUS3 protein levels...

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Identification of Direct Targets of FUSCA3, a Key Regulator of Arabidopsis Seed Development

Cited by 205 publications

References 79 publications

The pivotal role of small non-coding RNAs in the regulation of seed development

The pivotal role of small non-coding RNAs in the regulation of seed development

Deciphering the Role of POLYCOMB REPRESSIVE COMPLEX1 Variants in Regulating the Acquisition of Flowering Competence in Arabidopsis

Inhibition of FUSCA3 degradation at high temperature is dependent on ABA signaling and is regulated by the ABA/GA ratio

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