The MADS-Domain Transcriptional Regulator AGAMOUS-LIKE15 Promotes Somatic Embryo Development in Arabidopsis and Soybean

Thakare, Dhiraj; Tang, Weining; Hill, Kristine; Perry, Sharyn E.

doi:10.1104/pp.108.115832

Cited by 138 publications

(140 citation statements)

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“…Other genes have been identified that, when ectopically expressed, are able to promote somatic embryogenesis, including WUSCHEL (Gallois et al, 2004), BABY BOOM (Boutilier et al, 2002), SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KI-NASE1 (Hecht et al, 2001), LEAFY COTYLEDON 1-LIKE (Kwong et al, 2003) PGA37/MYB118 and MYB115 (Wang et al, 2009), and AGAMOUS-LIKE15 (AGL15; Harding et al, 2003;Thakare et al, 2008). Loss-of-function mutants in most of these genes do not have defects during zygotic embryo development, perhaps indicating the presence of redundant factors, as is common with ;90% of gene functions protected by redundancy (Pickett and Meeks-Wagner, 1995;Meinke et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…Ectopic expression of AGL15 promotes development of somatic embryos from zygotic embryo explants and then maintains development in this mode for extended periods (over 12 years to date; Harding et al, 2003). A 35S pro :AGL15 transgene also promotes somatic embryo development from the shoot apical region of seedlings that had completed germination in liquid media containing 2,4-D (Harding et al, 2003;Thakare et al, 2008). Furthermore, a putative ortholog of AGL15 from soybean (Glycine max) enhanced somatic embryo development in this species (Thakare et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…A 35S pro :AGL15 transgene also promotes somatic embryo development from the shoot apical region of seedlings that had completed germination in liquid media containing 2,4-D (Harding et al, 2003;Thakare et al, 2008). Furthermore, a putative ortholog of AGL15 from soybean (Glycine max) enhanced somatic embryo development in this species (Thakare et al, 2008). Although loss-of-function alleles of agl15 do not have any obvious impairment in zygotic embryo development, agl15, in some systems as a double mutant with a knockout allele in the closest family member agl18, showed decreased ability to produce somatic embryos (Thakare et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

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Global Identification of Targets of theArabidopsisMADS Domain Protein AGAMOUS-Like15

et al. 2009

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AGAMOUS-Like15 (AGL15) is a MADS domain transcriptional regulator that promotes somatic embryogenesis by binding DNA and regulating gene expression. Chromatin immunoprecipitation (ChIP) analysis previously identified DNA fragments with which AGL15 associates in vivo, and a low-throughput approach revealed a role for AGL15 in gibberellic acid catabolism that is relevant to embryogenesis. However, higher throughput methods are needed to identify targets of AGL15. Here, we mapped AGL15 in vivo binding sites using a ChIP-chip approach and the Affymetrix tiling arrays for Arabidopsis thaliana and found that ;2000 sites represented in three biological replicates of the experiment are annotated to nearby genes. These results were combined with high-throughput measurement of gene expression in response to AGL15 accumulation to discriminate responsive direct targets from those further downstream in the network. LEAFY COTYLE-DON2, FUSCA3, and ABA INSENSITIVE3, which encode B3 domain transcription factors that are key regulators of embryogenesis, were identified and verified as direct target genes of AGL15. Genes identified as targets of the B3 genes are also targets of AGL15, and we found that INDOLEACETIC ACID-INDUCED PROTEIN30 is involved in promotion of somatic embryo development. The data presented here and elsewhere suggest that much cross-regulation occurs in gene regulatory networks underpinning embryogenesis.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Global Identification of Targets of theArabidopsisMADS Domain Protein AGAMOUS-Like15

et al. 2009

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“…Ectopic expression of BBM results in the formation of somatic embryos and cotyledon-like structures on seedlings [15]. Moreover, overexpression of the Arabidopsis AGAMOUS-Like 15 (AGL15) gene and its homologous gene in soybean, which encode MADS domaincontaining proteins, enhances the formation of secondary embryos and somatic embryos [16,17]. The promotion of somatic embryo formation by AGL15 is attributed, at least in part, to a reduced GA level [18].…”

Section: Introductionmentioning

confidence: 99%

Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis

et al. 2008

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Formation of somatic embryos from non-germline cells is unique to higher plants and can be manipulated in a variety of species. Previous studies revealed that overexpression of several Arabidopsis genes, including WUSCHEL (WUS)/PLANT GROWTH ACTIVATOR6 (PGA6), BABY BOOM, LEAFY COTYLEDON1 (LEC1), and LEC2, is able to cause vegetative-to-embryonic transition or the formation of somatic embryos. Here, we report that a gain-offunction mutation in the Arabidopsis PGA37 gene, encoding the MYB118 transcription factor, induced vegetative-toembryonic transition, the formation of somatic embryos from root explants, and an elevated LEC1 expression level. Double mutant analysis showed that WUS was not required for induction of somatic embryos by PGA37/MYB118. In addition, overexpression of MYB115, a homolog of PGA37/MYB118, caused a pga37-like phenotype. A myb118 myb115 double mutant did not show apparent developmental abnormalities. Collectively, these results suggest that PGA37/ MYB118 and MYB115 promote vegetative-to-embryonic transition, through a signaling pathway independent of WUS.

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“…Other genes encoding transcription factors also promote embryo development when ectopically expressed, including BABY BOOM (BBM; Boutilier et al, 2002), WUSCHEL (Zuo et al, 2002;Gallois et al, 2004), LEAFY COTYLEDON1-LIKE (L1L; Kwong et al, 2003), PLANT GROWTH ACTIVATOR37/MYB118 and MYB115 , and AGAMOUS-LIKE15 (AGL15; Harding et al, 2003;Thakare et al, 2008). SOMATIC EMBRYO-GENESIS RECEPTOR-LIKE KINASE1 (Hecht et al, 2001) encodes a Leu-rich repeat receptor-like kinase, but it too promotes somatic embryo formation in some systems when expressed via the 35S promoter.…”

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confidence: 99%

Identification of Direct Targets of FUSCA3, a Key Regulator of Arabidopsis Seed Development

2013

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FUSCA3 (FUS3) is a B3 domain transcription factor that is a member of the LEAFY COTYLEDON (LEC) group of genes. The LEC genes encode proteins that also include LEC2, a B3 domain factor related to FUS3, and LEC1, a CCAAT box-binding factor. LEC1, LEC2, and FUS3 are essential for plant embryo development. All three loss-of-function mutants in Arabidopsis (Arabidopsis thaliana) prematurely exit embryogenesis and enter seedling developmental programs. When ectopically expressed, these genes promote embryo programs in seedlings. We report on chromatin immunoprecipitation-tiling array experiments to globally map binding sites for FUS3 that, along with other published work to assess transcriptomes in response to FUS3, allow us to determine direct from indirect targets. Many transcription factors associated with embryogenesis are direct targets of FUS3, as are genes involved in the seed maturation program. FUS3 regulates genes encoding microRNAs that, in turn, control transcripts encoding transcription factors involved in developmental phase changes. Examination of direct targets of FUS3 reveals that FUS3 acts primarily or exclusively as a transcriptional activator. Regulation of microRNA-encoding genes is one mechanism by which FUS3 may repress indirect target genes. FUS3 also directly up-regulates VP1/ABI3-LIKE1 (VAL1), encoding a B3 domain protein that functions as a repressor of transcription. VAL1, along with VAL2 and VAL3, is involved in the transition from embryo to seedling development. Many genes are responsive to FUS3 and to VAL1/VAL2 but with opposite regulatory consequences. The emerging picture is one of complex cross talk and interactions among embryo transcription factors and their target genes.

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The MADS-Domain Transcriptional Regulator AGAMOUS-LIKE15 Promotes Somatic Embryo Development in Arabidopsis and Soybean

Cited by 138 publications

References 44 publications

Global Identification of Targets of theArabidopsisMADS Domain Protein AGAMOUS-Like15

Global Identification of Targets of theArabidopsisMADS Domain Protein AGAMOUS-Like15

Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis

Identification of Direct Targets of FUSCA3, a Key Regulator of Arabidopsis Seed Development

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