The electrophoretic pattern of serum proteins in dairy cows with inflammatory diseases

Abstract

The objective of the study was to evaluate the electrophoretic pattern of serum proteins in dairy cows suffering from various infl ammatory diseases, and to study the infl uence of these diseases on the concentrations of protein fractions. Fourty dairy cows with clinical signs of various infl ammatory diseases were used in the study, including cows with post-partum metritis (n=10), mastitis (n=7), and hoof diseases (n=23). The cows were of a black pied Holstein-Friesian breed, Slovak spotted breed and their crossbreeds at the age of 3.5 to 8 years. Fourteen clinically healthy dairy cows were taken as the control group. The cows were blood sampled for the determination of total serum proteins and serum protein fractions. The protein fractions were divided into albumin, α 1 -, α 2 -, β 1 -, β 2 -, and γ-globulins. In cows with post-partum metritis we found signifi cantly lower concentrations of albumin (P<0.001) and signifi cantly higher values of α 1 -globulins (P<0.01) compared with healthy animals. Signifi cantly higher concentrations of β 1 -globulins were observed in cows with mastitis (P<0.001), while the γ-globulin fraction was non-signifi cantly higher. In cows with hoof diseases we recorded signifi cantly lower values of albumin (P<0.001), signifi cantly higher concentrations of α 1 -and β 1 -globulins (P<0.001), and non-signifi cantly higher β 2 -and γ-globulins. Moreover, in the electrophoretic pattern of serum proteins we found β-γ bridging in thirteen cows with hoof diseases. Presented data suggest marked infl uence of infl ammatory diseases on the concentrations of serum protein fractions in dairy cows. However, further investigations are needed to establish the diagnostic utility of serum protein electrophoresis in bovine clinical practice in relation to infl ammatory diseases.

Reproductive and Productive Performance of Dairy Cows After Lameness Treatment

“…Protein profile showed significant increase P˂0.05 in total protein and globulin levels in the treated group compared to control (7.83 ± 0.027, 6.94 ± 0.03 and 3.64 ± 0.013, 2.71 ± 0.033 respectively). These results agreed with Tóthová et al (2017) andJawor et al (2008) they reported a significant increase in serum total proteins and globulin levels in cows suffering from hoof and limb inflammatory diseases compared to control. Our results showed non-significant difference between studied groups in serum albumin level.…”

Abnormalities of Serum Protein Electrophoresis in Cattle and Comparison between Assays for Markers of Inflammation and Markers of Liver Alteration

“…1 and Fig. 2), the α2 and γ globulins are good markers of inflammation like this explained by Tóthová et al (2017). The case in Fig.…”

“…With this in mind, we have opted for the study of serum protein electrophoresis (SPE) to confirm its use as a means of diagnostics complementary to clinical examinations (Tóthová et al 2017), rather than the dosage of inflammation proteins (acute phase proteins of inflammation): haptoglobin and fibrinogen (Humblet et al 2004;Tóthová et al 2015;Salem 2017) which are used in Bovine medicine and which also remains expensive.…”

Dynamics of some indicators of blood serum in case of digestive disordersin calves in the early postnatal period

“…Normal albumin concentrations and high globulin concentrations have been shown to indicate hypoalbuminemia masked by dehydration [12,13]. Based on the fact that globulins are a group of proteins that are regulators of the immune system and simultaneously non-specific protease inhibitors [5,12,13], an increase in their concentration is an indicator of stimulation of the body's defenses against the background of organic and functional disorders of the gastrointestinal tract of calves, accompanied by diarrhea. Against the background of diarrhea in calves, the glucose level reached 5.83±0.93 mmol/l in the control group, and 4.85±0.84 mmol/l in the experimental group.…”