The Effects of Varying Solvent Polarity on Extraction Yield of Orthosiphon stamineus Leaves

Razak, Mohd Farhan B. Abdul; Yong, Pin Kar; Shah, Zamree Md; Abdullah, Luqman Chuah; Yee, Soh Sin; Yaw, Ir Thomas Choong She

doi:10.3923/jas.2012.1207.1210

Cited by 39 publications

(20 citation statements)

References 5 publications

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“…The result suggests that the major phytochemicals in L. cyanescens, leaves were mostly high in polarity and soluble in water. This is similar to the observation of Mohd Farhan et al, 45 with Orthosiphon stamineus. Although both water and methanol contain hydroxyl group that can form hydrogen bonding with the solute, water may be more effective in extracting the solute because it has higher polarity and shorter chain.…”

Section: Discussionsupporting

confidence: 81%

Evaluation of Lens Aldose Reductase Inhibitory and Free Radical Scavenging Potential of Fractions of Lonchocarpus cyanescens: Potential for Cataract Remediation

Ajani¹,

Sabiu²,

Odufuwa³

et al. 2016

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Section: Discussionsupporting

confidence: 81%

Evaluation of Lens Aldose Reductase Inhibitory and Free Radical Scavenging Potential of Fractions of Lonchocarpus cyanescens: Potential for Cataract Remediation

Ajani¹,

Sabiu²,

Odufuwa³

et al. 2016

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“…This is due to the variation in solubility and polarity of both component and solvent. Razak et al (2012) also reported that the extraction of O. stamineus gave the highest yield using water (34%) followed by ethanol (5%). It was stated that rosmarinic acid is present in O. stamineus abundantly of which it extraction is favoured by very polar solvents.…”

Section: Resultsmentioning

confidence: 93%

“…Water extract gave a comparable yield of 4.32 ± 0.03% which indicates that polar compounds are easier to be extracted compared to nonpolar compounds. Water, methanol and ethanol contain hydroxyl group which can form a hydrogen bonding with the solute, but water has shorter chain and higher polarity making it more effective in extracting the solute (Pin et al 2010;Razak et al 2012). Thus, the addition of water in the organic solvent increased the extraction yield for both ethanolic and methanolic solvent.…”

Section: Resultsmentioning

confidence: 99%

Cosolvent Selection for Supercritical Fluid Extraction (Sfe) of Bioactive Compounds from Orthosiphon stamineus

Mohamed@Mahmood¹,

Daud²,

Markom³

et al. 2018

JSM

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In this work, a preliminary study was conducted to study the effects of different types and concentrations of cosolvents based on the total yield and antioxidants capacity prior to supercritical fluid extraction (SFE) of Orthosiphon stamineus (locally referred as misai kucing). Initially, a comparison was made by cold maceration technique with nine types of different cosolvents, namely water, pure ethanol, 25% (v/v) of ethanol in water, 50% (v/v) of ethanol in water, 75% (v/v) of ethanol in water, pure methanol, 25% (v/v) of methanol in water, 50% (v/v) of methanol in water and 75% (v/v) of methanol in water. The antioxidant capacity was analysed by free radical scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH), total phenolic content (TPC) and total flavonoid content (TFC). Aqueous ethanolic solvent of 50% (v/v) ethanol in water showed the highest total yield of extract of 4.64 ± 0.02%. All antioxidant assays of TPC and TFC showed the highest value of 3.42 ± 0.08 mg GAE g −1 extract, 4.7 ± 0.14 mg CAE g −1 extract, respectively and IC 50 value for DPPH was 0.625 µg/mL for 50% (v/v) ethanol in water extract. Based on the overall result, ethanolic solvents gave a better result for all antioxidant assays compared to those of methanolic solvents. Using the selected cosolvent, the identification of target compounds, which were rosmarinic acid, eupatorin and sinensetin from supercritical fluid extraction was determined by using HPLC. In conclusion, ethanol-water solvent was efficient in extracting bioactive compounds in O. stamineus and also improved the total yield, thus the usage of ethanolic solvent in different concentrations should be considered for further optimisation of SFE with cosolvent studies.

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“…If the solubility value has almost the same value, then the compound will be easier to dissolve. The total solubility parameters include polar, nonpolar, and hydrogen bonds [28]. This study also used a method that searched for solvents according to the total solubility parameters based on the polarity of solvents, such as n-hexane (polar), ethyl acetate (semipolar), and water (polar).…”

Section: Discussionmentioning

confidence: 99%

Antibacterial Effects of Sarang Semut (Myrmecodia Pendans) Fractions Using Three Different Solvents Toward Enterococcus Faecalis Cps2

et al. 2020

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Objective: This study investigated the effect of antibacterial activity from sarang semut fractions with three different solvents, i.e. nonpolar (n-hexane),semipolar (ethyl acetate), and polar (water), to determine the minimum inhibitory concentration (MIC) on Enterococcus faecalis cps2.Methods: The fractions were extracted with a maceration method and a methanol solvent. The fractionation was performed with three groupsof solvent to obtain the n-hexane, ethyl acetate, and water fractions. The active compound from the best fraction group was identified using aphytochemical test, gas chromatography-mass spectrophotometry, and thin-layer chromatography. Each fraction group was divided into five differentconcentrations, i.e. 20%, 40%, 60%, 80%, and 100% and was assessed against E. faecalis cps2 with an agar diffusion method. Chlorhexidine 2% wasused as a positive control. The width of the inhibition zone was calculated.Results: The ethyl acetate group had the biggest inhibition zone of 21 mm in diameter compared to n-hexane and water, which was 15 mm and19 mm in diameter, respectively. The MIC value of the fraction with a 20% concentration of ethyl acetate was significantly different (P < 0.05) from then-hexane and water solvents in inhibiting the growth of E. faecalis cps2.Conclusion: The ethyl acetate fraction of sarang semut had a greater inhibitory effect on E. faecalis cps2. In addition, the antibacterial activity of thefraction increased with an increase in concentration.

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The Effects of Varying Solvent Polarity on Extraction Yield of Orthosiphon stamineus Leaves

Cited by 39 publications

References 5 publications

Evaluation of Lens Aldose Reductase Inhibitory and Free Radical Scavenging Potential of Fractions of Lonchocarpus cyanescens: Potential for Cataract Remediation

Evaluation of Lens Aldose Reductase Inhibitory and Free Radical Scavenging Potential of Fractions of Lonchocarpus cyanescens: Potential for Cataract Remediation

Cosolvent Selection for Supercritical Fluid Extraction (Sfe) of Bioactive Compounds from Orthosiphon stamineus

Antibacterial Effects of Sarang Semut (Myrmecodia Pendans) Fractions Using Three Different Solvents Toward Enterococcus Faecalis Cps2

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