The effects of quercetin on the expression of SREBP-1c mRNA in high-fat diet-induced NAFLD in mice

Abstract

Objectives The study aimed to determine the effect of quercetin on the expression of primary regulator gene involved in lipogenesis and triglycerides synthesis in the liver, and the sterol regulatory binding protein-1c (SREBP-1c) mRNA in non-alcoholic fatty liver disease (NAFLD) with a high-fat diet (HFD) model. Methods Fifty-six Balb/c mice were divided into seven groups: standard feed; HFD; HFD and quercetin 50 mg/kg for 28 days; HFD and quercetin 100 mg/kg BW for 28 days; HFD and quercetin 50 mg/kg for 14 days; HFD and quercetin 100 mg/kg for 14 days; HFD and repaired fed for 14 days. Quercetin was administered intraperitoneally. The animals were sacrificed 24 h after the last treatment; the liver was taken for macroscopic, histopathological staining using hematoxylin–eosin and reverse transcription-PCR analysis sample. Results HFD significantly increased the expression of SREBP-1c mRNA; meanwhile, quercetin and repaired feed significantly reduced the expression of SREBP-1c mRNA in the liver. Quercetin at a dose of 50 mg/kg and 100 mg/kg also improved liver cells’ pathological profile in high-fat diet NAFLD. Conclusions The present study suggests that quercetin has an inhibitory effect on SREBP-1c expression and improved liver pathology in NAFLD mice.

Orally administered octacosanol improves liver insulin resistance in high-fat diet-fed mice through the reconstruction of the gut microbiota structure and inhibition of the TLR4/NF-κB inflammatory pathway

“…39 Srebp-1c plays a central role in lipogenesis, enhancing the transcription of genes required for fatty acid syntheses, including fat acid synthase (Fas) and acetyl-CoA carboxylase 1 (Acc1). 40 Fas is a key enzyme controlling the rate of fatty acid synthesis, 41 while Acc1 is the rate-limiting enzyme in the biogenesis of long-chain fatty acids and regulation of fatty acid synthesis. 42 Our results showed that compared to the HFD mice, the Octa-treated mice showed significantly altered mRNA expressions of the above-mentioned genes, revealing the possibility of altered lipid metabolism through changes in the lipogenic genes caused by Octa in the HFD-fed mice.…”

The Role of Hydrogen Sulfide Regulation of Autophagy in Liver Disorders

“…It is highly expressed in liver [ 51 , 52 ]. The increasing evidence have shown that SREBP-1c contributes to NAFLD [ 53 , 54 , 55 ]. HFD significantly increased the expression level of SREBP-1c and its downstream lipid metabolism-related proteins, and downregulated autophagy in HFD-fed mice.…”

A review of edible plant-derived natural compounds for the therapy of liver fibrosis

“…Quercetin also improves DMN, TAA, MCD or CdCl 2 -induced liver fibrosis [80,210–213]. In HFD-fed rats or mice, quercetin protects liver damage via inhibiting SREBP-1c expression, modulating intestinal microbiota, or upregulating inositol-requiring enzyme 1 alpha/spliced X-box binding protein 1 pathway [214–216]. Quercetin could also alleviate autoimmune liver damage in ConA-induced mouse model by suppressing TRAF6/JNK pathway [217].…”