The effects of platelet-rich plasma on the osteogenic induction of bone marrow mesenchymal stem cells

Zou, Jun; Yuan, Chenxi; Wu, Chunshen; Cao, Cheng; Yang, Huilin

doi:10.3109/03008207.2014.930140

Cited by 32 publications

(28 citation statements)

References 18 publications

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“…These cultured ADSCs also maintained their differentiation potential, and were able to differentiate into adipocytes and osteoblasts… With these phenotypes, obtained ADSCs satisfied the minimal standard depicted by Dominici et al (Dominici et al, 2006). Similar studies have been successful in replacing FBS with PRP in MSC culture conditions, including those from as bone marrow (Amable et al, 2014;Zou et al, 2014), and umbilical cord blood Van Pham and Kim Phan, 2014).…”

Section: Discussionmentioning

confidence: 77%

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Pham¹,

Vu²,

Le³

et al. 2014

Biomed Res Ther

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Abstract-Adipose-derived stem cells (ADSCs) are excellent for regenerative medicine. Like mesenchymal stem cells, ADSCs possess multi-potent differentiation capacity that enables them to differentiate into osteoblasts, chondrocytes and adipocytes, as well as trans-differentiation into other cells. ADSC transplantation has gained attention in recent years, especially in vitro expanded ADSC transplantation. This study aimed to provide a new method to in vitro primarily culture and secondary culture of ADSCs that were compliant with good manufacturing practice for clinical applications. Stromal vascular fraction (SVF) was extracted from adipose tissue by commercial kits. SVF was expanded in vitro in medium with non-allogeneic supplements. Cultured ADSCs maintained immune-phenotype, karyotype, and differentiation potential after ten passages. Moreover, ADSCs at 15th passage could not form tumors in NOD/SCID mice. This research produced a suitable protocol for clinical applications of expanded ADSCs.

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Section: Discussionmentioning

confidence: 77%

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Pham¹,

Vu²,

Le³

et al. 2014

Biomed Res Ther

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“…Since we have no data regarding activated platelets (P-selectin-positive platelets), the exact influence of active platelets on the circulating CD34-positive cells is unknown. However, a previous study reported that platelet rich plasma enhances the proliferation of bone marrow multi-potent stem cells [6]. Therefore, we believe that the number of platelets correlates with the number of active platelets.…”

Section: Discussionmentioning

confidence: 83%

“…Additionally, plasma levels of S1P have been shown to lead to the trafficking of stem cells from the bone marrow to the peripheral blood [5]. Platelets are a rich source of VEGF, SDF-1α, and S1P [3], and platelet-rich plasma could enhance the proliferation of bone marrow mesenchymal stem cells, which are known to be multi-potent stem cells [6]. On the other hand, stem cells are also known to differentiate into megakaryocytes and to subsequently produce platelets [7].…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, platelets are reported to induce differentiation of human CD34-positive cells into endothelial cells [9]. Since CD34-positive cells have been reported to contribute to endothelial repair [10], and platelet rich plasma could enhance the proliferation of bone marrow multi-potent stem cells [6], high levels of platelets in the plasma might have a beneficial effect on endothelial repair. However, platelets are also reported to play an important role in the development of atherosclerotic lesions as an initial actor [11].…”

Section: Introductionmentioning

confidence: 99%

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Platelets as an indicator of vascular repair in elderly Japanese men

et al. 2016

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Platelets and circulating CD34-positive cells have been reported to contribute to vascular repair (endothelial repair and developing atherosclerosis). And because hypertension is known to be a strong vascular impairment factors, it should also influence the respective numbers of these factors. To clarify the clinical importance of platelets on vascular repair, we conducted a cross-sectional study of 567 Japanese men aged 60-69 who underwent an annual health check-up between 2013 and 2015. Multiple linear regression analysis of non-hypertensive subjects adjusting for classical cardiovascular risk factors showed that although platelet count did not significantly correlate with carotid intima media thickness (β = −0.05, p = 0.356), it did positively correlate significantly with the natural log of the number of circulating CD34-positive cells (β = 0.26, p < 0.001). In hypertensive subjects, a significant positive correlation was seen between platelets and intima media thickness (β = 0.19, p = 0.008), whereas no significant correlation was seen between platelet count and the natural log of the number of circulating CD34-positive cells (β = 0.11, p = 0.119). Our results indicate that platelet count is an indicator of vascular repair activity (endothelial repair and developing atherosclerosis). Additionally, hypertension might mask the beneficial effects of circulating CD34-positive cells.

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“…However, BMSCs in bone marrow are rare, with only one BMSC detected in 1 × 10 5 bone marrow mononuclear cells. PRP, as a storage vehicle for growth factors such as PDGF, TGF-β, PDEGF, VEGF, and platelet factor-4, enhances osteogenic differentiation of BMSCs [15]. Many studies have shown that factors that positively affect bone formation and fracture healing also positively affect tendon–bone healing [16].…”

Section: Introductionmentioning

confidence: 99%

Combination of platelet-rich plasma and bone marrow mesenchymal stem cells enhances tendon–bone healing in a rabbit model of anterior cruciate ligament reconstruction

Teng

Zhou

et al. 2016

J Orthop Surg Res

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BackgroundThe objective of this study was to investigate the potency of platelet-rich plasma (PRP) combined with bone marrow mesenchymal stem cells (BMSCs) to promote tendon–bone healing in a rabbit model.MethodsIn the in vitro study, the effects of PRP on osteogenic induction of BMSCs were analysed. Later, PRP with or without BMSCs was used in the rabbit model of anterior cruciate ligament reconstruction. Specimens were harvested 8 weeks postoperatively to evaluate tendon–bone healing by histology, radiology, and biomechanical testing.ResultsThe in vitro study revealed that collagen I, osteocalcin, and osteopontin expression was higher in BMSCs co-cultured with PRP for 14 days. The in vivo study revealed a more mature tendon–bone interface using light microscopy, a more newly formed bone at the bone tunnel walls detected by micro-computed tomography, and a significantly higher failure load as assessed by biomechanical testing in the BMSC + PRP group than in the control and PRP groups.ConclusionsThese results indicate that the combination of PRP and BMSCs promotes tendon–bone healing and has potential for clinical use.Electronic supplementary materialThe online version of this article (doi:10.1186/s13018-016-0433-7) contains supplementary material, which is available to authorized users.

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The effects of platelet-rich plasma on the osteogenic induction of bone marrow mesenchymal stem cells

Cited by 32 publications

References 18 publications

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Good manufacturing practice-compliant isolation and culture of human adipose derived stem cells

Platelets as an indicator of vascular repair in elderly Japanese men

Combination of platelet-rich plasma and bone marrow mesenchymal stem cells enhances tendon–bone healing in a rabbit model of anterior cruciate ligament reconstruction

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