The Effects of Light and Moderate Intensity Exercise on the Femoral Bone and Cerebellum of<scp>d</scp>-Galactose-Exposed Rats

Partadiredja, Ginus; Karima, Nisa; Utami, Kurnia Putri; Agustiningsih, Denny; Sofro, Zaenal Muttaqien

doi:10.1089/rej.2018.2050

Cited by 8 publications

(6 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with prior studies 5 , 44 – 46 , an impairment of trabecular bone microarchitecture following either D-gal injection or HFD consumption was discovered in our study. This structural change was detected early, at week 4, through the simultaneous loss of bone volume fraction (i.e.…”

Section: Discussionsupporting

confidence: 93%

D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis

Imerb

Thonusin

Pratchayasakul

et al. 2022

Sci Rep

View full text Add to dashboard Cite

We aimed to compare the time-course effect of D-galactose (D-gal)-induced aging, obesity, and their combined effects on bone homeostasis. Male Wistar rats were fed with either a normal diet (ND; n = 24) or a high-fat diet (HFD; n = 24) for 12 weeks. All rats were then injected with either vehicle or 150 mg/kg/day of D-gal for 4 or 8 weeks. Blood was collected to measure metabolic, aging, oxidative stress, and bone turnover parameters. Bone oxidative stress and inflammatory markers, as well as bone histomorphometry were also evaluated. Additionally, RAW 264.7 cells were incubated with either D-gal, insulin, or D-gal plus insulin to identify osteoclast differentiation capacity under the stimulation of receptor activator of nuclear factor κB ligand. At week 4, D-gal-induced aging significantly elevated serum malondialdehyde level and decreased trabecular thickness in ND- and HFD-fed rats, when compared to the control group. At week 8, D-gal-induced aging further elevated advanced glycation end products, increased bone inflammation and resorption, and significantly impaired bone microarchitecture in HFD-fed rats. The osteoclast number in vitro were increased in the D-gal, insulin, and combined groups to a similar extent. These findings suggest that aging aggravates bone dyshomeostasis in the obese condition in a time-dependent manner.

show abstract

Section: Discussionsupporting

confidence: 93%

D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis

Imerb

Thonusin

Pratchayasakul

et al. 2022

Sci Rep

View full text Add to dashboard Cite

show abstract

“…The precision estimate of the stereological procedure was calculated using the formula:

{CV}_{total}^{2} = {CV}_{biol}^{2} + {CE}^{2}

where CV is coefficient of variation, CV biol is biological coefficient of variation which reflects individually biological variations, and CE is coefficient of error that reflects sampling error (Boyce, Dorph‐Petersen, Lyck, & Gundersen, ; West, ). The sampling strategy is considered to be optimal if CE 2 /CV 2 is between 0.2 and 0.5 (Agustina, Sofro, & Partadiredja, ; Partadiredja, Karima, Utami, Agustiningsih, & Sofro, ).…”

Section: Methodsmentioning

confidence: 99%

Chlorogenic acid ameliorates memory loss and hippocampal cell death after transient global ischemia

Hermawati

Arfian

Mustofa

et al. 2019

Eur J of Neuroscience

Self Cite

View full text Add to dashboard Cite

Chlorogenic acid (CGA) is known to have antioxidant potentials, yet the effect of CGA on brain ischemia has not been sufficiently understood. Brain ischemia such as transient global ischemia disrupts many areas of the brain of rats, including the hippocampus. Male Wistar rats were randomly assigned into five groups, that is, sham‐operated (SO), bilateral common carotid occlusion (BCCO), and BCCO+ 15, 30, and 60 mg/kg bw CGA groups (CGA15, CGA30, and CGA60, respectively). Brain ischemia was induced in Wistar rats with BCCO for 20 min followed by intraperitoneal injection of CGA. The rats were examined for the spatial memory in a Morris water maze test on the 3rd day and were euthanized on the 10th day after BCCO. The total number of pyramidal cells was estimated, and the mRNA expressions of Bcl2, Bax, caspase‐3, SOD2, SOD1, GPx, ET‐1, eNOS, CD31, and VEGF‐A were measured. The BCCO group spent less time and distance in the target quadrant than any other group in the spatial memory retention test. The CA1 pyramidal cell numbers in the BCCO and CGA15 groups were lower than in the CGA30 and CGA60 groups. The mRNA expressions of Bcl2, SOD2, and CD31 in the BCCO group were lower than in the CGA15, CGA30, and CGA60 groups. The ET‐1 expression was higher in the BCCO and CGA15 groups than in the SO, CGA30, and CGA60 groups. CGA improves the spatial memory and prevents the CA1 pyramidal cell death after BCCO by increasing Bcl2, SOD2, and CD31 expressions and decreasing ET‐1 expression.

show abstract

“…The present study aimed to investigate the effect of low and moderate treadmill exercise on liver of d ‐Galactose‐exposed aging rat model. The present study focusing on liver fibrosis, number of M1 and M2, and M1/M2 ratio was an extension of a previous study by Partadiredja et al ().…”

Section: Introductionmentioning

confidence: 73%

Effects of low and moderate treadmill exercise on liver ofd‐galactose‐exposed aging rat model

et al. 2019

View full text Add to dashboard Cite

Aging increases liver susceptibility to diseases and it causes inflammation in liver tissue which can lead to fibrosis. Studies suggest that aging is caused by the accumulation of free radicals. Lack of physical activity can lower hormone levels and increase free radicals that can accelerate the aging process. Hence, physical activity is very important to maintain functions of organs. This research was aimed to study the effects of low and moderate treadmill exercise on d‐Galactose‐exposed aging rat model by evaluating the degree of hepatic fibrosis, number of M1 and M2, and M1/M2 ratio. Twenty‐four 3‐month‐old male Wistar aging model rats were randomly divided into four groups, that is, three treatment groups with daily 300 mg kgBW−1 d‐Galactose injection administrated intraperitoneally for 4 weeks and 1 control group with normal saline injection. Two of the d‐Galactose treated groups were given low and moderate treadmill exercise for 4 weeks. It was concluded that low intensity treadmill exercise significantly lowered the degree of d‐Galactose‐exposed hepatic fibrosis, and moderate treadmill exercise was able to restore the injured liver tissue back to the non‐aging state. Administration of d‐Galactose causes inflammation marked by the elevated number of M1 and M2 macrophages. Moderate treadmill exercise drove M1/M2 ratio back to the control condition.

show abstract

The Effects of Light and Moderate Intensity Exercise on the Femoral Bone and Cerebellum ofd-Galactose-Exposed Rats

Cited by 8 publications

References 54 publications

D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis

D-galactose-induced aging aggravates obesity-induced bone dyshomeostasis

Chlorogenic acid ameliorates memory loss and hippocampal cell death after transient global ischemia

Effects of low and moderate treadmill exercise on liver ofd‐galactose‐exposed aging rat model

Contact Info

Product

Resources

About