The effects of elevated temperatures on yeast. I. Nutrient requirements for growth at elevated temperatures

Sherman, Frederick S.

doi:10.1002/jcp.1030540105

Cited by 24 publications

(5 citation statements)

References 16 publications

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“…The change in streptomycin requirement with temperature might be analogous to the increased nutritional requirements reported for other microorganisms at elevated temperature (Hills and Spurr, 1952;Giles, Partridge, and Nelson, 1957;Sherman, 1959). In E. coli, a mutant requiring pantothenic acid only at a high temperature was shown to possess a thermolabile pantothenate-synthesizing enzyme functional only below the optimal temperature for the wild type (Maas and Davis, 1952).…”

Section: Discussionmentioning

confidence: 61%

A TEMPERATURE-SENSITIVE STREPTOMYCIN REQUIREMENT IN AN ESCHERICHIA COLI MUTANT

Plunkett¹

1962

J Bacteriol

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PLUNKETT, GEOFFREY E. (Biochemical Research Foundation, Newark, Del.). A temperature-sensitive streptomycin requirement in an Escherichia coli mutant. J. Bacteriol. 84:275-277. 1962.-A strain of Escherichia coli, streptomycin-dependent at 37.5 C, has been found to be capable of continuing cell division in the absence of streptomycin when incubated at 31 C. This property appeared to be heritable and persisted after repeated single-colony isolations from streptomycin-containing agar maintained at 37.5 C, or from streptomycin-free agar maintained at 31 C. The cells were stored under refrigeration on streptomycin-free agar slants, and retained their requirement for streptomycin

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Section: Discussionmentioning

confidence: 61%

A TEMPERATURE-SENSITIVE STREPTOMYCIN REQUIREMENT IN AN ESCHERICHIA COLI MUTANT

Plunkett¹

1962

J Bacteriol

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“…One is led to conclude, therefore, that the temperature-sensitive character of this psychrophil is probably explained by the presence of certain exceptionally thermolabile enzymes. Such enzymes have not been reported in psychrophils to date (Ingraham & Bailey, 1959); they have, however, been found in mutant strains of certain mesophilic micro-organisms (Horowitz & Fling, 1956 ;Maas & Davis, 1952), and there is also evidence to suggest that certain enzymes in wild-type mesophils may have relatively low temperature maxima (Lichstein & Begue, 1960;Sherman, 1959) and that these enzymes may form the basis of the temperature-sensitive system that is responsible for the onset of synchronous growth following repeated changes in incubation temperature (Eichel, 1956).…”

Section: Discussionmentioning

confidence: 95%

Studies on the Biochemical Basis of the Low Maximum Temperature in a Psychrophilic Cryptococcus

Hagen

Rose

1962

Journal of General Microbiology

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SUMMARYA psychrophilic species of Cryptococcw, freshly inoculated cultures of which grew well in a glucose +salts +vitamins medium at 16" but not at a,ll at 30°, was induced to grow rapidly for a period at 30" by previous incubation at 16". A study was made of certain aspects of the biochemical composition of the cryptococcus in cultures maintained at 16" as compared with that in cultures transferred from 16" to 30°, in order to obtain information about the biochemical basis of the low maximum growth temperature for this organism. With the cryptococcus transferred from 16' to 30°, there was rapid utilization of intracellular reserves of total amino acids and total 0x0 acids. Exogenous sources of amino acids were unable to replenish the intracellular amino acid pool in the cryptococcus incubated at 30". However, addition of a-oxoglutarate, citrate, or DL-isocitrate to cultures transferred to 30" after 120 hr. at 1 6 ' enabled the cryptococcus to continue to grow at the higher temperature; stimulation of growth at 80" was most marked in cultures supplemented with a-oxoglutarate. Other tricarboxylic acid cycle and related intermediates (pyruvate, acetate, succinate, fumarate, t-malate, oxaloacetate) had no growth-promoting effect. Stimulation of growth at 30" in the presence of a-oxoglutarate was obtained only in cultures which had been incubated for 2 4 days at 16", indicating the existence of other temperature-sensitive metabolic processes in addition to those concerned in a-oxoglutarate synthesis.

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“…Inactivation of inesophilic inicroorganisms a t teillperatures above ca. 60" C is usually thought to be a result of the denaturation of enzymes a t these higher temperatures; evidence to support this view comes froin the finding that some mesop1~ilic microorganisms become more exacting nutritionally a t higher temperatures, a result, presuiiably, of the thermal inactivation of certain synthesizing enzymes (2,17). It is possible, therefore, that psychrophilic behavior in microorganisins may be a reflection of the thermal inactivation of certain enzymes at comparatively low temperatures of approximately 30" C. The presence of an abnormally therinolabile pantothenatesynthesizing enzyme has been demonstrated in a mutant of Esche~ichia coli (12), while Horowitz and Fling have reported differences between wild strains of iVez~rospora with regard to the tl~ermostability of the enzyme tyrosinase (7).…”

Section: Discussionmentioning

confidence: 99%

A Psychrophilic Cryptococcus

Hagen¹,

Rose²

1961

Can. J. Microbiol.

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A psychrophilic yeast, isolated from decomposing Lantinaria found on the beach a t Hebron Fjord in Labrador, was shown to be a species of Cryptococczis, closely resembling but not identical with C. mucorugoszis and C. terricolus. I n a glucose-salts-vitamins medium, the yeast grew most rapidly a t 25' C, although the final cell crop was greatest in cultures incubated a t 21' C. The yeast grew well a t 3' C after a lag period of approximately five days, but no growth was observed in cultures incubated throughout a t 30° C or above. Cultures of the yeast which had been incubated a t 30° C, but not a t 37O C, grew, on being transferred to 16' C, after a lag period that was proportional to the duration of incubation a t 30° C. The yeast grew well a t 30° C after cultures had been incubated for a period a t 16' C, although growth ceased a t this higher temperature after a few days; further growth a t 30' C could be obtained after the cultures had again been incubated a t 16O C. The significance of these results is discussed in relation t o the psychrophilic behavior of the yeast.'Manuscript

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The effects of elevated temperatures on yeast. I. Nutrient requirements for growth at elevated temperatures

Cited by 24 publications

References 16 publications

A TEMPERATURE-SENSITIVE STREPTOMYCIN REQUIREMENT IN AN ESCHERICHIA COLI MUTANT

A TEMPERATURE-SENSITIVE STREPTOMYCIN REQUIREMENT IN AN ESCHERICHIA COLI MUTANT

Studies on the Biochemical Basis of the Low Maximum Temperature in a Psychrophilic Cryptococcus

A Psychrophilic Cryptococcus

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