A TEMPERATURE-SENSITIVE STREPTOMYCIN REQUIREMENT IN AN ESCHERICHIA COLI MUTANT

Abstract

PLUNKETT, GEOFFREY E. (Biochemical Research Foundation, Newark, Del.). A temperature-sensitive streptomycin requirement in an Escherichia coli mutant. J. Bacteriol. 84:275-277. 1962.-A strain of Escherichia coli, streptomycin-dependent at 37.5 C, has been found to be capable of continuing cell division in the absence of streptomycin when incubated at 31 C. This property appeared to be heritable and persisted after repeated single-colony isolations from streptomycin-containing agar maintained at 37.5 C, or from streptomycin-free agar maintained at 31 C. The cells were stored under refrigeration on streptomycin-free agar slants, and retained their requirement for streptomycin

Interrelationship Between Temperature and Sodium Chloride on Growth of Lactic Acid Bacteria Isolated From Meat-Curing Brines

“…A number of investigators have observed a requirement for certain vitamins and cofactors when microorgansims are cultured at high temperatures, and, if the temperature is decreased, the requirement for an exogenous supply of the cofactor no longer exists (Mitchell and Houlahan 1946a, b;Begue and Lichstein, 1959;Maas, 1950). Recently, this generalization was extended to a streptomycin-dependent strain of Escherichia coli that could grow in the absence of the antibiotic at lower temperatures (Plunkett, 1962). These results are in accord with the hypothesis that a heat-labile enzyme system is involved, and circumvention of the inhibition is accomplished by decreasing the temperature of incubation.…”