The effects of different (pseudo)halide substrates on peroxidase‐mediated killing of <i>Actinobacillus actinomycetemcomitans</i>

Ihalin, Riikka; Loimaranta, Vuokko; Lenander-Lumikari, M.; Tenovuo, Jorma

doi:10.1111/j.1600-0765.1998.tb02338.x

Cited by 39 publications

(23 citation statements)

References 41 publications

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“…Incorporation of I − and SCN − into peroxidase systems have been poorly investigated and contradictory results have been reported about the antimicrobial action of this combination; some are supporters of synergistic, the others, antagonistic interactions. For example, Ihalin and group successively reported that the addition of SCN − into the peroxidase/H 2 O 2 /I − system abolished the bactericidal activity of the oxidized halide (Ihalin et al, 1998, 2001). Similarly, Ahariz and Courtois (2010) demonstrated a competition between I − and SCN − , reporting that the addition of SCN − to peroxidase/G/GOD/I − system (G/GOD: glucose/glucose oxidase as source of hydrogen peroxide) decreased the antifungal effect of the system.…”

Section: Discussionmentioning

confidence: 99%

“…However, a peroxidase exerts an antimicrobial effect indirectly by catalyzing the transformation of a substrate with low antimicrobial properties into one with high antimicrobial effects (Klebanoff, 1967). The complete antimicrobial peroxidase system requires three components: a particular peroxidase enzyme, hydrogen peroxide (H 2 O 2 ), and an oxidizable substrate such as a halide or a pseudohalide (Ihalin et al, 1998). Peroxidase-catalyzed oxidation of (pseudo)halides yields reactive agents which oxidize microorganisms, damaging essential structural and functional components and causing inhibition of microbial metabolism and growth (Thomas and Fishman, 1986).…”

Section: Introductionmentioning

confidence: 99%

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Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems

et al. 2017

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Antibiotic resistance is an increasingly serious threat to global health. Consequently, the development of non-antibiotic based therapies and disinfectants, which avoid induction of resistance, or cross-resistance, is of high priority. We report the synthesis of a biocidal complex, which is produced by the reaction between ionic oxidizable salts—iodide and thiocyanate—in the presence of hydrogen peroxide as an oxidation source. The reaction generates bactericidal reactive oxygen and iodine species. In this study, we report that the iodo-thiocyanate complex (ITC) is an effective bactericidal agent with activity against planktonic and biofilm cells of Gram-negative (Escherichia coli and Pseudomonas aeruginosa) and Gram-positive (Staphylococcus aureus and methicillin-resistant S. aureus) bacteria. The minimum bactericidal concentrations and the minimum biofilm eradication concentrations of the biocidal composite were in the range of 7.8–31.3 and 31.3–250 μg ml−1, respectively. As a result, the complex was capable to cause a rapid cell death of planktonic test cultures at between 0.5 and 2 h, and complete eradication of dual and mono-species biofilms between 30 s and 10 min. Furthermore, the test bacteria, including a MRSA strain, exposed to the cocktail failed to develop resistance after serial passages. The antimicrobial activity of the ITC appears to derive from the combinational effect of the powerful species capable of oxidizing the essential biomolecules of bacteria. The use of this composition may provide an effective and efficient method for killing potential pathogens, as well as for disinfecting and removing biofilm contamination.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Antibacterial Potential of an Antimicrobial Agent Inspired by Peroxidase-Catalyzed Systems

et al. 2017

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“…[81012–14] Most studies use spectrophotometry to detect MPO levels. Azurophilic granules can be easily stained, and this test has been used extensively in Chédiak-Higashi syndrome.…”

Section: Discussionmentioning

confidence: 99%

Myeloperoxidase staining in the diagnosis of aggressive periodontitis

Singh¹,

Acharya²,

Kumar³

2011

J Indian Soc Periodontol

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Aims:To evaluate neutrophil myeloperoxidase (MPO) staining procedure as a reliable, affordable and easily available diagnostic assay for aggressive periodontitis.Materials and Methods:Fifteen subjects were recruited in the study wherein five each were diagnosed as aggressive periodontitis and chronic periodontitis respectively, and five were periodontally healthy. Three millilitres (ml) of venous blood was collected using Vacutainers containing ethylene diamine tetra acetate (EDTA) and was subjected to MPO staining procedure. Histological picture was evaluated using a visual analogue scale (VAS).Results:MPO stained specimen of all the patients showed positive MPO staining of the neutrophils. The intensity of the stain of MPO granules was more in aggressive periodontitis specimen as compared to the chronic periodontitis patient specimen and healthy subject specimen. The staining characteristics were comparable for chronic periodontitis patients and healthy subject.Conclusions:This study shows that there is a potential and probable place for MPO staining as an economical, relatively convenient and easily available assay in the diagnosis of aggressive periodontitis.

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“…The peroxidase-SCN − system has antibacterial effects against many bacteria, including streptococci and lactobacilli, and some periodontal and other mucosal pathogens (Pruitt and Reiter, 1985;Tenovuo, 1985;Courtois et al, 1992;Ihalin et al, 1998). In streptococci it inhibits glucose incorporation, glycolysis, and acid production by cariogenic bacteria (Pruitt and Reiter, 1985;Lenander-Lumikari and Loimaranta, 2000); it also affects the cytoplasmic membrane and inhibits various respiratory oxidases and reductases in E. coli.…”

Section: Peroxidasesmentioning

confidence: 99%