The effects of diazinon on lipid peroxidation and antioxidant enzymes in rat erythrocytes: role of vitamins E and C

Abstract

Reactive oxygen species caused by organophosphates may be involved in the toxicity of various pesticides. Therefore, in this study, we aimed to investigate the effects of acute exposure to organophosphate insecticide diazinon (DI) and possible ameliorating role of vitamins E and C, with the following parameters: lipid peroxidation (LPO) and the activity of the glutathione peroxidase (GSH-Px) and superoxide dismutase (SOD) in rat erythrocytes. The experimental groups were arranged as control group, DI-treated group (DI) and DI + vitamin E + vitamin C-treated group (DI + Vit). DI + Vit groups were treated orally with a single dose of 335 mg/kg DI body weight. Vitamins E and C were injected at doses of 150 mg/kg body weight intramuscular (in) and 200 mg/kg body weight intraperitoneal (ip), respectively, 30 min after the treatment of DI in DI + Vit group. Blood samples were taken 24 h after the DI. The results showed that DI administration caused to increase in LPO and the activities of SOD and GSH-Px enzymes in erythrocytes. Also, the combination of vitamins E and C decreased LPO and the activities of GSH-Px and SOD compared with the DI group. In conclusion, although treating rats with single dose DI increases LPO and antioxidant enzyme activities in erythrocytes, vitamins C and E combination can reduce LPO caused by DI.

Amelioration of liver and kidney functions disorders induced by sodium nitrate in rats using wheat germ oil

“…The ability of animals to resist the toxic effects of environmental agents is dependent on the detoxication and antioxidant systems. Recently, several nutrients and other chemicals are effective antioxidants, such as vitamins, trace elements, amino acids and their derivatives, fatty acids and plant phenolics (Ayo, Minka, & Mamman, 2006;Son, Mo, Rhee, & Pyo, 2004;Suteu et al, 2007).…”

Protective Effect of Tert Butyl Hydroquinone on Diazinon-Induced Oxidative Stress in Brain and Heart of Male Rats

“…In recent years, some agents with antioxidant effects (e.g. vitamins C and E, N-acetylcysteine and zinc) have been used to decrease cellular oxidative stress and thus cellular damage, in cases of organophosphate poisoning [18,25]. Tert-butylhydroquinone (TBHQ) is one of the few antioxidants permitted for use in foods.…”

“…The severity of diazinon-induced lipid peroxidation in different tissues depends on several factors such as oxygen consumption, metabolic activity rate and susceptibility to oxidants [29]. The use of antioxidants has been suggested as one of the methods of dealing with the toxicity of organophosphates in various studies [18,25,26,31]. TBHQ is a derivative of hydroquinone, substituted with tert-butyl group.…”

“…Administration of diazinon to rats resulted in depletion of glycogen from the brain and peripheral tissues [17]. It has also been shown that diazinon caused an increase in lipid peroxidation in rat erythrocytes [18]. Diazinon treatment in rats decreased renal antioxidants and enhanced lipid peroxidation with concomitant renal damage, which are involved in the diazinon-induced renal oxidative stress and toxicity [19].…”

The Protective Role of Vitamin C against Formaldehyde inducedhepatotoxicity and nephrotoxicity in Male Rats

“…Some vitamins (such as vitamins A, E, and C) are known to play an important role in ameliorating the toxicity effects of reactive species generated by chemical agents in biological systems. Vitamins C and E are known to be potent antioxidants [19,20].…”