The effects of desferrioxamine on cisplatininduced lipid peroxidation and the activities of antioxidant enzymes in rat kidneys

Abstract

<jats:p> Cisplatin-induced nephrotoxicity is associated with an increase in lipid peroxidation and oxygen free radicals in rat kidneys. In this study, the effects of desferrioxamine were compared to vitamin C and E on cisplatin-induced lipid peroxidation and antioxidant enzyme activities in rat kidneys. Rats were divided into five groups, with 15 Wistar rats in each group. In the control group, rats received 1 mL/100 g isotonic saline solution intraperitoneally (i.p.). In Group II, 10 mg/kg cisplatin i.p. was injected to rats. Thirty minutes before the same dosage of cisplatin administration, 100 mg/kg i.p. vitamin C or E was given to rats in groups III and IV, respectively. Rats in Group V received 250 mg/kg desferrioxamine i.p., before the same dose of cisplatin administration. All rats were killed by cervical dislocation after 72 hours. The kidneys were immediately removed and washed in cold saline. Spectrophotometric method was used for all analyses. While catalase, glutathione reductase (GR), and super oxide dismutase (SOD) levels were found to be significantly decreased (P B &lt; 0.001), malondialdehyde (MDA) (P &lt; 0.05) and hydrogen peroxide (H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub>) (P &lt; 0.001) levels were significantly increased in the cisplatin group when compared to the controls. MDA levels were decreased by desferrioxamine (P &lt; 0.005) as well as vitamin C and E (P &lt; 0.05 and P &lt; 0.001, respectively). These three compounds induced a significant increase in SOD levels (P B &lt; 0.05), but only in the vitamin C group, were SOD levels not significantly different than the levels of the controls (P &gt; 0.05). In the desferrioxamine (P &lt; 0.05), vitamin C and E groups (P &lt; 0.001 for both), the cisplatin elevated H<jats:sub>2</jats:sub>O<jats:sub>2</jats:sub> levels were decreased. None of these drugs had any effect on GR and catalase levels (P &gt; 0.05). Desferrioxamine is useful to prevent cisplatin-induced lipid peroxidation, however, vitamin C and E are more effective on antioxidant enzymes than desferrioxamine. </jats:p>

Plant Food Extracts as Source of Bioactive Compounds for Prevention of Cisplatin-Induced Kidney Dysfunction in Rats

“…Results from the current work further support reports from several studies that provided evidence that the cellular events in cisplatin-mediated nephrotoxicity are a consequence of reactive oxygen species (ROS) generation, which produces oxidative renal damage [Yilmaz et al, 2004;Kadikoylu et al, 2004]. Cisplatin in the present study produced significant reduction of the activities of plasma catalase (an antioxidant enzyme), plasma total antioxidant capacity accompanied by increased lipid peroxidation compared with the control rats.…”

Antioxidant effect of carnosine pretreatment on cisplatin-induced renal oxidative stress in rats

“…In fact, there are evidences of the renal increase in NO production in cisplatin nephrotoxicity (27). The O 2 •-increase in cisplatin-nephrotoxicity may be simply consequence of the mitochondrial dysfunction (4) and the decrease in superoxide dismutase activity (28). One of the most important intracellular antioxidant systems is the glutathione redox cycle.…”

The Protective Effects of Amifostine on Adriamycin-Induced Acute Cardiotoxicity in Rats

“…Amifostine decreases generation of free oxygen radicals and might have a protective effect on adriamycin toxicity (Singal and Tong 1988;Dorr 1996;Kang et al 1996;Samelis et al 1998;Kocak et al 2003;Kadikoylu et al 2004). Adriamycin-induced hematological and testicular toxicities, cataract formation, and cisplatininduced neuro-, nephro-and ototoxicities may be either prevented or decreased by administration of amifostine before cytotoxic chemotherapy in experimental and clinical studies (Spencer et al 1995;Santini and Giles 1999).…”