The effects of cryopreservation on sperm morphology, motility and mitochondrial function

O’Connell, Michael P.; McClure, Neil; Lewis, Sheena

doi:10.1093/humrep/17.3.704

Cited by 390 publications

(257 citation statements)

References 27 publications

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“…14 Yet another study showed that the progressively motile sperms were found to decrease by 41% and the motile sperms by 33% after freezing and thawing. 11 However, we could not find any study in the past that was found to study the effect of motility in the prolonged post thaw interval. So we went a step ahead, waited for 40 min, while incubating the post wash swim up at 37 C and then analysed it.…”

Section: Discussionmentioning

confidence: 91%

“…10 Therefore; an impairment of mitochondrial activity may explain the reduction in motility. 11 Although motility is not directly related to the fertilizing capacity, it is one of the most important factors affecting the sperm quality. 12 According to Keel and Webster, 1993, it has been documented that the motility of sperms is found to decrease by 50% after the freeze thaw cycle.…”

Section: Discussionmentioning

confidence: 99%

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Study of human sperm motility post cryopreservation

Oberoi

Kumar

Talwar

2014

Medical Journal Armed Forces India

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a b s t r a c tBackground: Cryopreservation of spermatozoa is a widely used technique to preserve the fertility of males. It can also benefit the armed forces personnel who are to be sent for long recruitments, while leaving their families behind. This study, apart from studying the effects of freezing and thawing, reveals the effect of the post thaw interval on the motility of the human spermatozoa and thus widens the insemination window period.Methods: A detailed semen analysis was carried out as per the WHO guidelines for 25 samples. The samples were then washed, analysed and frozen in liquid nitrogen. The semen samples were subsequently thawed and similarly analysed after 20 min and 40 min of thawing. This was then followed by statistical analysis of the comparative motilities.Results: Motility of sperms is found to decrease after cryopreservation. However, the study revealed that after thawing a significant increase in the motility of the sperms was noted with the progression of time (p < 0.05). Conclusion:By simulating conditions similar to the in vivo conditions for the post thaw semen samples, we can safely wait, confirm the parameters like motility and count, and then inseminate the samples instead of blindly inseminating them immediately after thawing.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Study of human sperm motility post cryopreservation

Oberoi

Kumar

Talwar

2014

Medical Journal Armed Forces India

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“…During the process of freezing and thawing, physical and chemical stress to the sperm membrane are produced, leading to loss of sperm viability and fertilizing ability [2]. ROS are produced by a wide variety of exogenous chemicals and metabolic processes causing a broad spectrum of damage to the biological system.…”

Section: Discussionmentioning

confidence: 99%

“…Cryopreservation of human semen represents a useful therapeutic option in the management of infertility with several indications [1]. However, during cryopreservation, spermatozoa are exposed to physical and chemical stress that results in adverse changes in membrane lipid composition, sperm motility, viability and acrosome status [2][3][4][5][6]. All these changes reduce the fertilizing ability of human spermatozoa after cryopreservation [7].…”

Section: Introductionmentioning

confidence: 99%

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Martínez-Soto¹,

DiosHourcade²,

Gutiérrez‐Adán³

et al. 2010

Asian J Androl

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In this study, we evaluated the effects of genistein supplementation of the thawing extender on frozen-thawed human semen parameters. We analyzed the effect of supplementation on sperm motility, capacitation (membrane lipid disorder), reactive oxygen species (ROS) generation, chromatin condensation and DNA damage. Using this preliminary information, it maybe possible to improve the cryopreservation process and reduce the cellular damage. We have confirmed that the isoflavone genistein (10 mmol L -1 ) has antioxidant properties on the frozen-thawed spermatozoa. This results in a decreased ROS production that shows a slight improvement in the sperm motility, and decreases the membrane lipid disorder and DNA damage caused by cryopreservation. These results suggest an effect of genistein on sperm functionality that could be of interest for assisted reproduction treatments using frozenthawed human spermatozoa, but further studies will be necessary to confirm our findings and to evaluate the possible clinical applications.

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“…Motility depends on endogenous ATP to transport chemical energy, and reduced motility may be associated with mitochondrial damage (O'Connell et al, 2002). Carp (Cyprinus carpio) sperm motility quits when 50 to 80% of ATP is exhausted via hydrolysis (Billard et al, 1995).…”

Section: Sperm Quality Parametersmentioning

confidence: 99%

Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Jenkins

Olivier

Draugelis-Dale

et al. 2014

Science of The Total Environment

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The effects of cryopreservation on sperm morphology, motility and mitochondrial function

Abstract: Sperm morphology, motility, mitochondrial activities and viability are equally susceptible to cryopreservation-induced damage. R123 intensity is a novel and robust indicator of mitochondrial function before and after such trauma.

Cited by 390 publications

References 27 publications

Study of human sperm motility post cryopreservation

Study of human sperm motility post cryopreservation

Effect of genistein supplementation of thawing medium on characteristics of frozen human spermatozoa

Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

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