Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Jenkins, Jill A.; Olivier, Heather M.; Draugelis-Dale, Rassa O.; Eilts, B.E.; Torres, Leticia; Patiño, Reynaldo; Nilsen, Elena B.; Goodbred, Steven L.

doi:10.1016/j.scitotenv.2013.09.097

Cited by 21 publications

(10 citation statements)

References 86 publications

(107 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A high intersex incidence (between 80 and 100% of male fish) has also been found in some areas of the United Kingdom and United States. Studies of wild roach (Bjerregaard et al, 2006;Jobling et al, 2009;Jobling et al, 1998), and largemouth bass (Micropterus salmoides) (Blazer et al, 2007(Blazer et al, , 2012(Blazer et al, , 2014 found that intersex severity was dependent upon the density of human and animal populations in the watershed. We observed the same trend in this study, where the most densely populated area (Kitchener) was correlated with the highest score of intersex severity.…”

Section: Reproductive Healthmentioning

confidence: 99%

“…Thus, many potentially harmful compounds enter the receiving environment via MWWTP effluent. Feminization of male fish is one impact of MWWE exposure, and has been noted in wild fish populations throughout the western world (Jobling et al, 1998;Bjerregaard et al, 2006;Blazer et al, 2007;Tetreault et al, 2011). Male fish show various indicators of feminization including smaller testes, lower levels of male sex hormones, reduced secondary sexual characteristics, presence of immature oocytes in testes (intersex) and elevation of plasma vitellogenin (VTG) levels (Desforges et al, 2010;Liney et al, 2005;Tetreault et al, 2011;Vajda et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

“…The most thoroughly studied populations of feminized male fish are the roach (Rutilus rutilus) in UK rivers, where the degree of feminization correlates with the density of MWWTPs (Jobling et al, 1998). Feminized roach have been characterized as having smaller gonads, intersex condition, and high plasma VTG levels (Jobling et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Feminized roach have been characterized as having smaller gonads, intersex condition, and high plasma VTG levels (Jobling et al, 1998). When studies were conducted to determine the reproductive fitness of these fish, feminized males displayed decreased gamete quality (lower sperm density) and diminished fertilization success (Jobling et al, 2002a,b).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

et al. 2015

View full text Add to dashboard Cite

Section: Reproductive Healthmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

et al. 2015

View full text Add to dashboard Cite

“…In addition to cell membrane integrity, other sperm cell characteristics can be evaluated using flow cytometry, such as: mitochondrial activity (Ogier De Baulny et al, 1997, Trigo et al, 2015), ATP content (Ogier De Baulny et al, 1999), apoptosis, and nuclear DNA fragmentation (Jenkins et al, 2014). However, in small fish, such as biomedical models, the volume of sperm that can be collected is minimal (≤ 2 ul), and the possibility of additional quality assessment is typically not possible.…”

Section: Introductionmentioning

confidence: 99%

Amine reactive dyes: An alternative to estimate membrane integrity in fish sperm cells

Torres

Tiersch

2016

Aquaculture

View full text Add to dashboard Cite

Fluorescent dyes that binds irreversibly to cellular amines, come in several available emission spectra, and do not poses health concerns were used to evaluate membrane integrity in fish sperm cells. The objectives of the present study were to determine: (1) a working dye concentration for fish sperm samples, and (2) if the traditional propidium iodide/SYBR-14 staining combination was comparable with the amine reactive dye (ARD) methods at identifying cell populations with intact and compromised membranes after sperm activation, refrigerated storage, and exposure to cryoprotectant and surfactant. Zebrafish (Danio rerio) sperm were obtained by stripping, and pooled samples (in triplicate) were used in all tests. Six dilutions of the amine dye (ranging from 0.625 to 0.02 μL/mL) were evaluated, and compared with the traditional staining protocol. A concentration of 0.5 μL/mL ARD was selected to be used in subsequent assays. Sperm suspensions were activated with deionized water to simulate urine contamination. After 10 sec, osmolality was increased to stop activation, and the procedure was repeated in 10-sec intervals until the sperm remained activated for 120 consecutive sec; membrane integrity was analyzed at each time interval. For the storage assay, sperm suspensions were prepared in Hanks’ balanced salt solution at 302 mOsm/kg osmolality (HBSS302), HBSS354 and HBSS402, and evaluated every 2 hr for 8 hr, and every 24 hr for 72 hr. Cryoprotectant toxicity was tested by diluting sperm suspensions in HBSS340 with methanol at 5, 10 and 15% final concentrations. Surfactant toxicity was tested by diluting sperm suspensions in HBSS354 with Triton X-100 at 0.2, 0.15 and 0.1 mM final concentrations. In each toxicity assay, membrane integrity was tested every 20 min for 80 min. The number of membrane-intact cells significantly decreased across time in all treatments (p < 0.05). Significant differences between staining protocols were observed after activation and after exposure to methanol at 10 and 15%, and to Triton X-100 (p < 0.05). The average difference, however, was minor (between 1 and 6% in average) in relation to the typical values used for decision making based on this assay. Results showed that this method has the potential to contribute greatly to the standardization of cryopreservation in aquatic species.

show abstract

Estrogenicity and androgenicity screening of PCB sulfate monoesters in human breast cancer MCF-7 cells

Flor

Lehmler

et al. 2015

Environ Sci Pollut Res

View full text Add to dashboard Cite

Recent studies identified PCB sulfate esters as a major product of PCB metabolism. Since hydroxy-PCBs (HO-PCBs), the immediate precursors of PCB sulfates and important contributors to PCB toxicity, were shown to have estrogenic activity, we investigated the estrogenicity/androgenicty of a series of PCB sulfate metabolites. We synthesized the five possible structural sulfate monoester metabolites of PCB 3, a congener shown to be biotransformed to sulfates, a sulfate ester of the paint-specific congener PCB 11, and sulfate monoesters of two HO-PCBs reported to interact with sulfotransferases (PCB 39, no ortho chlorines, and PCB 53, 3 ortho chlorines). We tested these PCB sulfates and 4’-HO-PCB 3 as positive control for estrogenic, androgenic, anti-estrogenic and anti-androgenic activity in the E- and A-screen with human breast cancer MCF7 derived cells at 100 μM – 1 pM concentrations. Only 4’-HO-PCB 3 was highly cytotoxic at 100 μM. We observed structure-activity relationships: compounds with a sulfate group in the chlorine-containing ring of PCB 3 (2PCB 3 and 3PCB 3 sulfate) showed no interaction with the estrogen (ER) and androgen (AR) receptor. The 4’-HO-PCB 3 and its sulfate ester had the highest estrogenic effect, but at 100 fold different concentrations, i.e. 1 μM and 100 μM, respectively. Four of the PCB sulfates were estrogenic (2’PCB 3, 4’PCB 3, 4PCB 39, 4PCB 53 sulfates; at 100 μM). These sulfates and 3’PCB 3 sulfate also exhibited anti-estrogenic activity, but at nM and pM concentrations. The 4’PCB 3 sulfate (para-para’ substituted) had the strongest androgenic activity, followed by 3’PCB 3, 4PCB 53, 4PCB11, and 4PCB 39 sulfates and the 4’HO-PCB 3. In contrast, anti-androgenicity was only observed with the two compounds that have the sulfate group in ortho- or meta- position in the second ring (2’PCB 3 and 3’PCB 3 sulfate). No dose-response was observed in any screen, but, with exception of estrogenic activity (only seen at 100 μM), endocrine activity was often displayed at several concentrations and even at 1 pM concentration. These data suggest that sulfation of HO-PCBs is indeed reducing their cytotoxicity and estrogenicity, but may produce other endocrine disruptive activities at very low concentrations.

show abstract

Assessing reproductive and endocrine parameters in male largescale suckers (Catostomus macrocheilus) along a contaminant gradient in the lower Columbia River, USA

Cited by 21 publications

References 86 publications

Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

Amine reactive dyes: An alternative to estimate membrane integrity in fish sperm cells

Estrogenicity and androgenicity screening of PCB sulfate monoesters in human breast cancer MCF-7 cells

Contact Info

Product

Resources

About