Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

Fuzzen, Meghan; Bennett, Charles H.; Tetreault, Gerald R.; McMaster, Mark E.; Servos, Mark R.

doi:10.1016/j.aquatox.2015.01.009

Cited by 52 publications

(55 citation statements)

References 39 publications

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“…Continuous up-regulation of choriogenin H minor in rainbow darter from DSW may suggest estrogenic exposure of fish, most likely originating from the Waterloo MWWTP which persisted in the river water where these fish resided. In several studies, it has been shown that intersex occurrence in the rainbow darter collected downstream of the Waterloo MWWTP was more prevalent compared with the upstream sites [15,31]. The vtg transcript was not elevated in rainbow darter in the present study, even though vtg induction has been commonly seen as a response to estrogens in wastewaterexposed sites [1,57].…”

Section: Selected Transcripts That Were Differentially Expressedcontrasting

confidence: 68%

“…In 2011 DSW fish showed a down-regulation in their "stress response," whereas in 2012 the liver was dominated by processes that were involved in apoptosis. Exposure of fish to municipal effluents can cause changes in sex steroid production, and decreased production of androgen steroids in particular has been reported in rainbow darter in the Grand River downstream of wastewater outfalls [15,25]. Previous studies showed that the process of apoptosis was associated with exposure of fish to municipal effluents [23].…”

Section: Gene Set Enrichment Analysis and Mwwtp Upgradesmentioning

confidence: 99%

“…Studies conducted in rivers throughout the United Kingdom have demonstrated the feminization of male fish and the presence of intersex (ova-testes) in wild populations of roach (Rutilus rutilus), which was associated with the proximity to municipal wastewater-treatment plants (MWWTPs) [ [1][2][3]. In addition to intersex, MWWTP effluents have been associated with vitellogenin induction in male fish [7,8], altered steroid hormone production [9,10], reduced secondary sex characteristics [11,12], disrupted behavior [13,14], impaired gametogenesis [15,16], and effects on somatic indices, including gonad somatic index (GSI) and liver somatic index (LSI) [8,17,18]. In addition to intersex, MWWTP effluents have been associated with vitellogenin induction in male fish [7,8], altered steroid hormone production [9,10], reduced secondary sex characteristics [11,12], disrupted behavior [13,14], impaired gametogenesis [15,16], and effects on somatic indices, including gonad somatic index (GSI) and liver somatic index (LSI) [8,17,18].…”

Section: Introductionmentioning

confidence: 99%

“…This species is highly abundant throughout the Grand River watershed, and it has a relatively small home range, moving only a few meters, mostly in the spring during spawning [26,27]. High incidence of intersex condition (testes-ova) [26,31], decreased production of androgens [25,26], and reduced reproductive performance [15] have been observed in rainbow darter downstream of the wastewater outfalls. Recent studies have shown that complex mixtures of pollutants released from the 2 major municipal wastewater outfalls in the central reaches of the Grand River (i.e., Kitchener and Waterloo) have resulted in endocrine disruption in rainbow darter.…”

Section: Introductionmentioning

confidence: 99%

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Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

Marjan

Martyniuk

Fuzzen

et al. 2017

Enviro Toxic and Chemistry

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The present study measured hepatic transcriptome responses in male rainbow darter (Etheostoma caeruleum) exposed to 2 municipal wastewater-treatment plants (MWWTPs; Kitchener and Waterloo) over 4 fall seasons (2011-2014) in the Grand River (Ontario, Canada). The overall goal was to determine if upgrades at the Kitchener MWWTP (in 2012) resulted in transcriptome responses indicative of improved effluent quality. The number of differentially expressed probes in fish downstream of the Kitchener outfall (904-1223) remained comparable to that downstream of Waterloo (767-3867). Noteworthy was that year and the interaction of year and site explained variability in more than twice the number of transcripts than site alone, suggesting that year and the interaction of year and site had a greater effect on the transcriptome than site alone. Gene set enrichment analysis revealed a gradual reduction in the number of gene ontologies over time at exposure sites, which corresponded with lower contaminant load. Subnetwork enrichment analysis revealed that there were noticeable shifts in the cell pathways differently expressed in the liver preupgrade and postupgrade. The dominant pathways altered preupgrade were related to genetic modifications and cell division, whereas postupgrade they were associated with the immune system, reproduction, and biochemical responses. Molecular pathways were dynamic over time, and following the upgrades, there was little evidence that gene expression profiles in fish collected from high-impact sites postupgrade were more similar to those in fish collected from reference site. Environ Toxicol Chem 2017;36:2108-2122. © 2017 SETAC.

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Section: Selected Transcripts That Were Differentially Expressedcontrasting

confidence: 68%

Section: Gene Set Enrichment Analysis and Mwwtp Upgradesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

Marjan

Martyniuk

Fuzzen

et al. 2017

Enviro Toxic and Chemistry

Self Cite

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“…There is substantial evidence that 41 exposure of male fish to WwTW effluents causes feminization, and that severely 42 feminized male gonads impair breeding success of those individuals. [1][2][3] Feminized male 43 phenotypes include the production of the female yolk protein precursor vitellogenin, 4 44 feminized reproductive ducts and the presence of both male and female germ cells in the 45 male gonad. 5 46 47 The estrogenic activity of WwTW effluents predominantly results from the presence of 48 steroid estrogens emanating from human excretion.…”

mentioning

confidence: 99%

Effects of Exposure to WwTW Effluents over Two Generations on Sexual Development and Breeding in Roach Rutilus rutilus

Hamilton

Lange

Nicol

et al. 2015

Environ. Sci. Technol.

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Exposure to environmental estrogens in wastewater treatment works (WwTW) effluents induces feminized responses in male fish, including the development of eggs in male testes. However, the impacts on the offspring of exposed fish are not well understood. In this study, we examined whether roach (Rutilus rutilus) from mothers that had been exposed to an undiluted WwTW effluent from early life to sexual maturity had altered susceptibility to gonadal feminization and an impaired capacity to reproduce. For males from both WwTW effluent exposed mothers and dilution water exposed mothers, effluent exposure for up to 3 years and 9 months induced feminized male gonads, although the intersex condition was relatively mild. There was no difference in the severity of gonadal feminization in roach derived from either WwTW effluent exposed or dilution water exposed mothers. Furthermore, a breeding study revealed that roach with effluent-exposed mothers reproduced with an equal success as roach with mothers exposed to clean water. Roach exposed to the effluent for 3 years in this study were able to reproduce successfully. Our findings provide no evidence for impacts of WwTW effluent exposure on reproduction or gonadal disruption in roach down the female germ line and add to existing evidence that male roach with a mild intersex condition are able to breed competitively.

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Nonlethal laparoscopic detection of intersex (testicular oocytes) in largemouth bass (Micropterus salmoides) and smallmouth bass (Micropterus dolomieu)

MacLeod

Blazer

Matsche

et al. 2017

Enviro Toxic and Chemistry

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Intersex in wild fish populations has received considerable attention in the scientific literature and public media. Conventional detection of testicular oocytes, the presence of immature oocytes within testis of male fish, commonly employs transverse sectioning of excised testis and is lethal. The present study used a nonlethal laparoscopic technique to collect biopsies of testis from black bass, entering the body cavity via the genital pore. Detection of testicular oocytes was compared between biopsy and conventional methods using 79 smallmouth bass (Micropterus dolomieu) from 8 sites and 68 largemouth bass (Micropterus salmoides) from 4 sites. The 2 methods performed similarly at sites where testicular oocyte severity was moderate or high (6 of 8 smallmouth bass sites), whereas transverse sectioning resulted in superior testicular oocyte detection at sites where severity was low (2 of 8 smallmouth bass sites and all 4 largemouth bass sites). In smallmouth bass, testicular oocyte prevalence by transverse and biopsy methods was strongly correlated across sites (r = 0.81) and severity reported by enumeration of testicular oocytes was moderately correlated across sites (r = 0.59). Survival of a subset of largemouth bass (n = 20) to 28 d after laparoscopic surgery was 90%. The present research indicates that laparoscopy may be useful for monitoring the prevalence and severity of testicular oocytes in Micropterus species, particularly when lethal sampling is precluded. Environ Toxicol Chem 2017;36:1924-1933. © 2016 SETAC.

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Severe intersex is predictive of poor fertilization success in populations of rainbow darter (Etheostoma caeruleum)

Cited by 52 publications

References 39 publications

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

Returning to normal? Assessing transcriptome recovery over time in male rainbow darter (Etheostoma caeruleum) liver in response to wastewater‐treatment plant upgrades

Effects of Exposure to WwTW Effluents over Two Generations on Sexual Development and Breeding in Roach Rutilus rutilus

Nonlethal laparoscopic detection of intersex (testicular oocytes) in largemouth bass (Micropterus salmoides) and smallmouth bass (Micropterus dolomieu)

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