The effects of cryopreservation on red blood cell microvesiculation, phosphatidylserine externalization, and CD47 expression

Holovati, Jelena L.; Wong, Kenneth A.; Webster, Jarret M.; Acker, Jason P.

doi:10.1111/j.1537-2995.2008.01735.x

Cited by 38 publications

(44 citation statements)

References 45 publications

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“…On the other hand, alterations in electrolyte levels were thought to have resulted from the rinsing series during deglycerolization process since intracellular K + flows out whereas extracellular Na + flows in through the cellular membrane during freezing and storage processes of RBCs, which eventually serves the electrolyte balance mechanisms (Hogman and Meryman 1999). The results of Holovati et al (2008), who pointed out the depletion of potassium during deglycerolization was compatible with our findings. It was a remarkable finding that Sup.…”

Section: Discussionsupporting

confidence: 92%

The effects of freezing on long-term storage of canine erythrocytes

Bala¹,

Özcan²

2016

Polish Journal of Veterinary Sciences

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Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells.Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained. The samples were prewashed three times in 0.9% NaCl solution and were allocated into three groups to be evaluated at three different time points (day 0 and month 4 and 6). The samples to be frozen were subjected to glycerolization and then stored at -80 o C for 4 and 6-month periods. At the end of this period the packed RBC samples were thawed, centrifuged and then washed in a consecutive series of dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), Adenosin triphosphate (ATP), supernatant hemoglobin (SupHb), sodium (Na + ) and potassium (K + ) levels, residual glycerol concentrations and hemograms were evaluated and compared. Sterility tests were performed on all samples for bacterial contamination. A statistically significant decrease was noted in potassium levels, which was the natural outcome of deglycerolization process. No significant change was observed in terms of other parameters due based on different time points. In conclusion, long-term frozen storage had no negative effect on the quality parameters of canine erythrocytes.

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Section: Discussionsupporting

confidence: 92%

The effects of freezing on long-term storage of canine erythrocytes

Bala¹,

Özcan²

2016

Polish Journal of Veterinary Sciences

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“…Since all metabolic and biochemical activities are suppressed within the cell during the freezing process (Holovati et al 2008), a decrease in ATP concentration is not to be expected. However, thawing haemolysis (Kim et al 2004) may cause a decrease in ATP levels, which is closely associated with that of 2,3-DPG (Hess and Greenwalt 2002;Holme 2005).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, it was also suggested that alterations in stored erythrocytes result not only from intracellular factors and storage conditions but also from contaminating leukocytes and their side products (Andreu 1991;Holovati et al 2008). Therefore, the necessity of a closed system processing unit ensuring stringent control against bacterial contamination was particularly underlined (Battaglia 2000;Lucas et al 2004).…”

mentioning

confidence: 99%

Freezing and storage of leukodepleted erythrocyte suspensions

Bala¹,

Eraslan²,

Akyazı³

et al. 2016

Vet. Med. - Czech

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Studies on the frozen storage of human blood products have benefited veterinary transfusion medicine in recent years, but the long-term cryopreservation of canine red blood cells (RBCs) has not yet been thoroughly investigated. Further, no studies are available with respect to the frozen storage of leukocyte-depleted canine red blood cells (LD-RBCs). The objective of the current study was to investigate time-dependent effects of long-term frozen storage on leukocyte-depleted canine RBCs. Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged for 5 min at 22 °C and 4200 × g in a cryogenic microcentrifuge and concentrated RBC (pRBC) suspensions were obtained. Leukocyte depletion was achieved by filtration (2.6 log 10 ). Then, the filtrated samples were prewashed three times in 0.9% NaCl solution and were allocated into three subgroups to be evaluated at three different time points (Day 0, Month 4 and Month 6). The samples for cryopreservation were subjected to glycerolisation and then stored at -80 °C for 4-and 6-month periods. At the end of this period pRBC units were thawed by manual agitation in a water bath maintained at 36-38 °C, centrifuged and then washed in a consecutive series of 12%, 1.6% and 0.9% of NaCl + 0.2 dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), adenosine triphosphate (ATP), supernatant haemoglobin (SupHb), sodium (Na + ) and potassium (K + ) levels, residual glycerol concentrations and haemograms of thawed and deglycerolised pRBC samples were evaluated together with those of Day 0. Sterility tests were performed on all samples for bacterial contamination. No statistically significant differences were noted except for Hct and SupHb levels. No bacterial contamination was noted in any of the samples on the basis of sterility tests. It was found that the described glycerolisation procedure could be a method of choice in the cryopreservation of leukocyte-depleted pRBCs (LD-pRBCs) since no negative effect was observed on the quality of the products and long-term frozen storage did not cause RBC destruction.

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“…Важливо відзначити, що в суспензіях розморожених еритроцитів постійно спостерігаються тран-сформовані клітини. Відомо, що везикуляція мембран еритроцитів може відбуватися як в стресових [18], так і в фізіологічних умовах, зокрема, в процесі старіння in vivo [19]. Та-ким чином клітини позбавляються від діля-нок мембрани, які несуть в собі специфічні ознаки старіння і можуть розпізнаватися іму-нокомпетентними клітинами, що дає змогу їм безпечно залишатися довгий час у руслі крові [19].…”

Section: схема дослідуunclassified

“…При гіпотермічному зберіганні еритроци-тів зміни структури плазматичної мембрани пов'язані з метаболічними порушеннями і окисними процесами, які торкаються ліпід-них і білкових компонентів мембрани [18]. Оскільки зберігання еритроцитів за умов гіпотермії до 10 діб несуттєво впливало на характеристики CD44, можна зробити ви-сновок, що цей період часу не є критичним для розвитку пошкоджень мембрани, які б позначалися на стабільності клітин, зокрема, не було виявлено сублетальних ушкоджень, пов'язаних з змінами характеристик CD44.…”

Section: схема дослідуunclassified

Changes in Erythrocyte Surface Marker Cd44 During Hypothermic and Low Temperature Storage

Zemlianskykh¹,

Babijchuk²

2016

Fiziol Zh

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The effects of cryopreservation on red blood cell microvesiculation, phosphatidylserine externalization, and CD47 expression

Cited by 38 publications

References 45 publications

The effects of freezing on long-term storage of canine erythrocytes

The effects of freezing on long-term storage of canine erythrocytes

Freezing and storage of leukodepleted erythrocyte suspensions

Changes in Erythrocyte Surface Marker Cd44 During Hypothermic and Low Temperature Storage

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