The effects of freezing on long-term storage of canine erythrocytes

Abstract

Human medicine studies have so far demonstrated that erythrocytes may be preserved and stored at low temperatures for decades retaining their metabolic and biochemical properties. However, detailed studies regarding this problem are not yet available in veterinary medicine. Therefore, the objective of the current study was to investigate time-dependent effects of long-term frozen storage of canine red blood cells.Twelve healthy adult dogs meeting the criteria for blood transfusion were used in the study. Whole blood samples (450 ± 45 ml) collected from each dog were centrifuged by a cryogenic microcentrifuge and packed RBC suspensions were obtained. The samples were prewashed three times in 0.9% NaCl solution and were allocated into three groups to be evaluated at three different time points (day 0 and month 4 and 6). The samples to be frozen were subjected to glycerolization and then stored at -80 o C for 4 and 6-month periods. At the end of this period the packed RBC samples were thawed, centrifuged and then washed in a consecutive series of dextrose solutions. 2,3-Diphosphoglycerate (2,3-DPG), Adenosin triphosphate (ATP), supernatant hemoglobin (SupHb), sodium (Na + ) and potassium (K + ) levels, residual glycerol concentrations and hemograms were evaluated and compared. Sterility tests were performed on all samples for bacterial contamination. A statistically significant decrease was noted in potassium levels, which was the natural outcome of deglycerolization process. No significant change was observed in terms of other parameters due based on different time points. In conclusion, long-term frozen storage had no negative effect on the quality parameters of canine erythrocytes.

Association of Veterinary Hematology and Transfusion Medicine (AVHTM) Transfusion Reaction Small Animal Consensus Statement (TRACS). Part 1: Definitions and clinical signs

“…Reports of bacterial contamination of blood units in veterinary medicine are less frequently described than in human medicine, likely because fresh platelet transfusions are less common in veterinary medicine. Percentage of negative bacteriological units was high (94-100%) in studies on feline [131][132][133][134][135][136] and canine blood and plasma products [137][138][139][140] .…”

To study the effect of oxygen carrying capacity on expressed changes of erythrocyte membrane protein in different storage times

“…The transport of oxygen in the body all depends on the affinity of hemoglobin with oxygen. In the case of high partial pressure of oxygen, oxygen is easy to combine with hemoglobin, while in the case of low partial pressure of oxygen, oxygen and hemoglobin are easily dissociated [17]. It can be seen from Figure 2A that the oxygen partial pressure of red blood cells decreased gradually with the extension of storage time, which indicated that the effective oxygen carrying and oxygen releasing capacity of red blood cells decreased.…”

Blood Hypothermic Storage and Erythrocyte Cryopreservation in Dogs

“…The slow cooling (40% glycerol concentration) showed quite a high effi ciency (70-80% survival) for canine erythrocytes [1].…”

“…Найбільш ефективними непроникними кріопротекторами для кріоконсервування еритроцитів собак є поліетиленгліколь з м. м. 1500 (ПЕГ-1500) [1,2] та гідроксиетильований крохмаль (ГЕК) [15,21,28,31]. Ці речовини ефективні в низьких концентраціях і забезпечують захист клітин за допомогою часткової їх дегідратації перед заморожуванням.…”