The effects of compound treatment of Aspergillus oryzae and fibrolytic enzyme on in vitro degradation, gas production and fermentative profile of maize silage and sugarcane silage

Abstract

The present study was conducted to evaluate the effect of a live culture of Aspergillus oryzae (A; CCT4359) and fibrolytic enzyme (E; Fibrozyme Alltech Inc.) on fibre digestibility by a gas production bioassay and in vitro degradation of maize silage and sugarcane silage. A completely randomized design trial was performed to evaluate: A doses (0, 20, 60 and 100 mg/l), E doses (0, 160, 320 and 480 mg/l) and roughage source (R; maize and sugarcane silage) in a 4 × 4 × 2 factorial arrangement. The inclusion of increasing doses of A and E increased dry matter and neutral detergent fibre in vitro digestibility linearly, but for E this effect occurred only in maize silage. There was a linear increase in the potential for gas production at the highest dose of A only in sugarcane silage, with no effect on lag time (L). Increasing doses of E increased the volume of gases produced linearly, and a trend of linear reduction of L, regardless of the roughage. There was a linear reduction in ammonia-nitrogen concentration in response to increasing doses of A and E, and an increase in acetic acid concentration at the highest dose of A, regardless of roughage. The additives had no synergistic effect on gas production and digestibility, but were efficient in altering the fermentative pattern, demonstrating the potential to increase fibre degradation.

Use of exogenous fibrolytic enzymes and probiotic in finely ground starters to improve calf performance

“…Wang et al 11 showed that supplementation with fibrolytic enzymes tended to increase feed intake of bull calves aged 46 to 90 days. The discrepancy between our results and those of other researchers could be due to the method of administration of fibrolytic enzymes (direct administration into the starter feed versus pretreatment of the forage with fibrolytic enzymes), the composition of the basal feed, especially fiber sources or fiber content, which affect the activity and stability of rumen enzymes 14 . The lower concentration of NDF and higher digestibility of NDF should improve feed intake.…”

“…Both supplements, fibrolytic enzymes and probiotics, have the potential to modulate rumen fermentation and improve fiber digestibility, animal performance, and feed conversion efficiency. Some researchers have investigated the synergistic interaction between these two additives in in vitro and in vivo studies 12 – 14 . Despite conflicting results in in vitro studies, Malik and Bandla 12 reported that the combination of probiotics and fibrolytic enzymes had a reciprocal beneficial effect on rumen fermentation and improved growth performance and feed efficiency of growing buffalo calves.…”