The effects of 50 Hz magnetic field–exposed cell culture medium on cellular functions in FL cells

Fei, Yue; Su, Liling; Lou, Haifeng; Zhao, Chuning; Wang, Yiqin; Chen, Guangdi

doi:10.1093/jrr/rrz020

Cited by 12 publications

(8 citation statements)

References 37 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There is not currently a consensus on the effect of static or alternating magnetic fields on cells. [61][62][63][64] Here, cells on non-actuated control devices were exposed to a static magnetic field from the embedded permanent magnet, but were not exposed to the time-varying magnetic field. Although we do not anticipate that the static or dynamic magnetic fields will affect breast cancer cell behavior, this is worth investigating in future studies which aim to more deeply explore the metabolic activity, matrix deposition, and mechanotransduction of metastatic cancer cells.…”

Section: Discussionmentioning

confidence: 99%

High‐Throughput Magnetic Actuation Platform for Evaluating the Effect of Mechanical Force on 3D Tumor Microenvironment

Enríquez

Libring

Field

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

Accurately replicating and analyzing cellular responses to mechanical cues is vital for exploring metastatic disease progression. However, many of the existing in vitro platforms for applying mechanical stimulation seed cells on synthetic substrates. To better recapitulate physiological conditions, a novel actuating platform is developed with the ability to apply tensile strain on cells at various amplitudes and frequencies in a high‐throughput multi‐well culture plate using a physiologically relevant substrate. Suspending fibrillar fibronectin across the body of the magnetic actuator provides a matrix representative of early metastasis for 3D cell culture that is not reliant on a synthetic substrate. This platform enables the culturing and analysis of various cell types in an environment that mimics the dynamic stretching of lung tissue during normal respiration. Metabolic activity, YAP activation, and morphology of breast cancer cells are analyzed within one week of cyclic stretching or static culture. Further, matrix degradation is significantly reduced in breast cancer cell lines with metastatic potential after actuation. These new findings demonstrate a clear suppressive cellular response due to cyclic stretching that has implications for a mechanical role in the dormancy and reactivation of disseminated breast cancer cells to macrometastases.

show abstract

Section: Discussionmentioning

confidence: 99%

High‐Throughput Magnetic Actuation Platform for Evaluating the Effect of Mechanical Force on 3D Tumor Microenvironment

Enríquez

Libring

Field

et al. 2020

Adv Funct Materials

View full text Add to dashboard Cite

show abstract

“…For instance, when a human renal cell line was exposed to a 1 mT, 10 Hz field, production of intracellular ROS was inhibited [ 77 ], while exposure of microglial cells to a 1 mT, 50 Hz field prevented elevation of ROS and calcium levels, as well as subsequent cell death [ 78 ]. Some studies have even found that NIR exposure did not elicit changes in ROS levels, such as in human amniotic epithelial cells exposed to a 50 Hz magnetic fields ranging from 0–4 mT [ 79 ]. For more comprehensive reviews on the different effects of magnetic fields on biological systems and radical pairs, see [ 80 , 81 ].…”

Section: Potentials Of Non-ionizing Radiation To Control Tissue Growthmentioning

confidence: 99%

An Open Question: Is Non-Ionizing Radiation a Tool for Controlling Apoptosis-Induced Proliferation?

Hack

Kinsey

Beane

2021

IJMS

View full text Add to dashboard Cite

Non-ionizing radiation is commonly used in the clinical setting, despite its known ability to trigger oxidative stress and apoptosis, which can lead to damage and cell death. Although induction of cell death is typically considered harmful, apoptosis can also be beneficial in the right context. For example, cell death can serve as the signal for new tissue growth, such as in apoptosis-induced proliferation. Recent data has shown that exposure to non-ionizing radiation (such as weak static magnetic fields, weak radiofrequency magnetic fields, and weak electromagnetic fields) is able to modulate proliferation, both in cell culture and in living organisms (for example during tissue regeneration). This occurs via in vivo changes in the levels of reactive oxygen species (ROS), which are canonical activators of apoptosis. This review will describe the literature that highlights the tantalizing possibility that non-ionizing radiation could be used to manipulate apoptosis-induced proliferation to either promote growth (for regenerative medicine) or inhibit it (for cancer therapies). However, as uncontrolled growth can lead to tumorigenesis, much more research into this exciting and developing area is needed in order to realize its promise.

show abstract

“…The ELF magnetic field is a component of ELF electromagnetic waves that have the ability to penetrate almost all materials. The interaction of the ELF magnetic field with cells is able to change the cell membrane potential, so that it will have an impact on biophysical mechanisms in cells [1]. This mechanism underlies the appearence of biological effects on living things including bacteria which are exposed to the ELF magnetic field.…”

Section: Introductionmentioning

confidence: 99%

The effectiveness of exposure to magnetic fields of extremely low frequency 300T and 500T in inhibiting the proliferation of pathogenic bacteria to increase physical resistance of vannamei shrimp

Sudarti

Qumairoh

Prihandono

2022

J. Phys.: Conf. Ser.

View full text Add to dashboard Cite

Vannamei shrimp is a type of fish that rots easily, only able to survive about 6 hours at room temperature. This study aims to examine the effectiveness of exposure to an Extremely Low Frequency (ELF) magnetic field with an intensity of 300 T and 500 T in inhibiting the proliferation of pathogenic bacteria to increase the physical resistance of white vaname shrimp. The research design used a completely randomized design and was carried out at the Physics Laboratory of the Faculty of Teacher Training and Education, University of Jember. The Extremely Low Frequency (ELF) magnetic field in this study is a component of the ELF electromagnetic wave generated by the Current Transformer by minimizing the exposure to the ELF electric field to about 30 V/m. The study sample was 210 fresh vaname shrimp and divided into 7 treatment groups, namely the control group (K), the group exposed to the ELF magnetic field intensity of 300 T for 60 minutes (E-300.60’), 90 minutes (E-300.90’), 120 minutes (E-300.120’), and the group exposed to an ELF magnetic field of 500 T intensity for 60 minutes (E-500.60’), 90 minutes (E-500.90’), 120 minutes (E-500,120’). As an indicator of vaname shrimp resistance at room temperature is the population of pathogenic bacteria, color, aroma, texture, mucus, and pH. The research data were analyzed using One Way Anova analysis. The results showed that the population of Salmonella bacteria was significantly lower than the control in the vannamei shrimp sample exposed to an ELF magnetic field of 500 T, but the population of E.Coli bacteria increased significantly in the vannamei shrimp sample exposed to an ELF magnetic field of 300 T and 500 T compared to control. Naturally, vaname shrimp stored at room temperature showed the appearance of the shrimp getting paler, and the texture getting softer, smelling rotten and slimy after the 6th hour. However, the color, smell, and texture of shrimp exposed to an ELF magnetic field of 500 T intensity for 120 minutes were able to survive at a score of 8 at the 5th hour observation and 7th at the 10th hour observation. Conclusion: Exposure to an ELF magnetic field with an intensity of 500 T was effective in suppressing the proliferation of salmonella Sp bacteria but was less effective in suppressing the proliferation of E.Coli bacteria. This condition affects the physical condition of white shrimp until it lasts at the 10th hour. The results of this study serve as the basis for developing food security technology by the ELF magnetic field.

show abstract

The effects of 50 Hz magnetic field–exposed cell culture medium on cellular functions in FL cells

Cited by 12 publications

References 37 publications

High‐Throughput Magnetic Actuation Platform for Evaluating the Effect of Mechanical Force on 3D Tumor Microenvironment

High‐Throughput Magnetic Actuation Platform for Evaluating the Effect of Mechanical Force on 3D Tumor Microenvironment

An Open Question: Is Non-Ionizing Radiation a Tool for Controlling Apoptosis-Induced Proliferation?

The effectiveness of exposure to magnetic fields of extremely low frequency 300T and 500T in inhibiting the proliferation of pathogenic bacteria to increase physical resistance of vannamei shrimp

Contact Info

Product

Resources

About