The effect of zinc aspartate pretreatment on ischemia-reperfusion injury and early changes of blood and tissue antioxidant enzyme activities after unilateral testicular torsion-detorsion

Özkan, Keramettin Uğur; Boran, Çetin; Kılınç, Metin; Garipardıç, Mesut; Kurutas, EB

doi:10.1016/j.jpedsurg.2003.09.013

Cited by 57 publications

(55 citation statements)

References 24 publications

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“…Similarly, SOD is an enzyme that functions to re-establish the balance after oxidative stress injury and, therefore, it may serve as an indicator for oxidative stress. 22 In previous studies, it's been shown that there were some histopathological changes and a decrease in testicular perfusion not only in ipsilateral torted, but also in the contralateral nontorted testis. 23,24 The agents used for prevention of I/R damage on ipsilateral testis also have beneficial effects on contralateral testis.…”

Section: Discussionmentioning

confidence: 95%

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An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

Kemahlı

Yildiz

Fırat

et al. 2016

CUAJ

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Introduction: The aim of this experimental study was to investigate the histopathological and biochemical effects of pyrrolidine dithiocarbamate, an antioxidant and inhibitor of NF-kβ, on ischemiareperfusion injury in rats. Methods: A total of 21 male Wistar-Albino rats were randomly distributed into three groups as sham group (Group 1), ischemia-reperfusion (I/R) group (Group 2) and I/R with pyrrolidine dithiocarbamate (PDTC) group (Group 3). Left testicles of rats in Groups 2 and 3 underwent testicular torsion of 720° for four hours and 100 mg/ kg of PDTC was administered intraperitoneally prior to detorsion in Group 3. An hour after detorsion process, left orchiectomies were performed and 5 ml of intracardiac blood samples were drawn from rats in all three groups. Histopathological examination of testis tissues performed and measurement of superoxide dismutase (SOD) and malondialdehyde (MDA) levels in blood samples were taken. Results: Elevated levels of MDA and decreased SOD activity, together with decreased Johnson tubular biopsy scores consistent with I/R injury were observed in Group 2 (p<0.05). Group 1 and Group 3 were similar in terms of MDA levels, SOD activity, and Johnson scores (p>0.05). Conclusions: Our results indicated that PDTC may have beneficial effects for alleviation of I/R injury in testicular tissue in rats. Understanding the underlying mechanisms and exploration of its diagnostic and therapeutic potential requires further randomized, controlled trials on a larger scale.

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Section: Discussionmentioning

confidence: 95%

“…Animals were kept at constant temperature (20)(21)(22) o C) and Test animal were divided ino three groups, with each group consisting of seven rats. Procedures administered in each group were as follows: Group 1 (control) underwent left orchiectomy after scrotal incision without any additional surgical interventions.…”

Section: Experimental Designmentioning

confidence: 99%

An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

Kemahlı

Yildiz

Fırat

et al. 2016

CUAJ

View full text Add to dashboard Cite

show abstract

“…[20][21] as well as the peroxidative damage induced by ischemia-reperfusion as a consequence testicular torsion-detorsion. 22 Zinc administration has also been shown to attenuate the testicular oxidative DNA damage induced by Figure 1. Major pathways of reactive oxygen species generation and metabolism.…”

Section: Small Molecular Mass Antioxidantsmentioning

confidence: 99%

Antioxidant Systems and Oxidative Stress in the Testes

Aitken

Roman

2009

Advances in Experimental Medicine and Biology

159

105

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“…TBARS levels decreased to approximately normal levels following ATP-MgCl( 2 ) administration before or after detorsion. Ozkan et al 24 studied the effect of zinc aspartate (ZA) pretreatment in testicular torsion/detorsion. Again TBARS level decreased following ZA administration.…”

Section: Discussionmentioning

confidence: 99%

Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis

Guimarães¹,

Kimura²,

Vasconcelos³

2010

Acta Cir. Bras.

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Purpose:To evaluate the protective role of dimethylsulfoxide (DMSO) in a rat model of testis ischemia/reperfusion (I/R). Methods: Twenty-four male Wistar rats were randomized in two equal groups. Control rats (G-1) received saline 2.0 ml intraperitoneally (ip) 21, 9 and 1 h before torsion. Experimental rats (G-2) received ip injections of 3% aqueous solution of DMSO, 0.1ml/10g body weight. Saline was added to complete 2.0ml when necessary. I/R injury was induced in anesthetized rats by torsion of the right testis lasting 3 hours. Testis and blood samples were collected at the end of ischemia (T-0) and 3 hours later (T-3) for assessment of testis malonaldehyde (MDA), reduced glutathione (GSH), and plasma total antioxidant power (TAP). Results: MDA levels decreased significantly in G-2 rats compared with G-1 animals in all time-points. GSH levels increased significantly in T-0 and T-3 time-points in DMSO pretreated rats compared with G-1 rats. GSH levels increased significantly during reperfusion in G-2 rats. TAP was similar in both groups denoting absence of systemic effects in this study. Conclusion: Pretreatment with DMSO reduces testis lipid peroxidation and oxidative stress caused by torsion/detorsion of the testis. Key words: Antioxidants. Testis. Oxidative Stress. Malondialdehyde. Glutathione. Rats. RESUMO Objetivo:Avaliar o papel protetor do dimetilsulfóxido (DMSO) utilizando um modelo de isquemia/reperfusão (I/R) do testículo do rato. Métodos: Vinte e quatro ratos machos Wistar, randomizados em dois grupos iguais e tratados com solução salina 2,0 ml (grupo Controle-G-1) ou solução de DMSO (3%, 0,1ml/10g peso corporal), acrescida de solução salina até completar 2,0 ml (grupo Experimento-G-2) por via intraperitoneal, as 21, 9 e 1 h antes da torção. .A lesão de I/R foi induzida por torção do testículo direito e mantida por 3 horas, em ratos previamente anestesiados. Amostras (sangue e testículo direito) foram coletadas ao término da isquemia (T-0) e após 3 horas de reperfusão (T-3) para determinação das concentrações de malonaldeído (MDA), glutationa reduzida (GSH) e capacidade antioxidante total do plasma (CAT). Resultados: As concentrações de MDA diminuíram significativamente nos ratos do G-2. comparados ao G-1, nos tempos estudados. As concentrações de GSH aumentaram significativamente nos testículos dos ratos do G-2 comparado aos controles no T-0 e no T-3 e durante a reperfusão no G-2. Os valores obtidos na análise do CAT foram semelhantes. Conclusão: O pré-tratamento com DMSO reduz a peroxidação lipídica e o estresse oxidativo decorrentes da torção/destorção do testículo, no rato.

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The effect of zinc aspartate pretreatment on ischemia-reperfusion injury and early changes of blood and tissue antioxidant enzyme activities after unilateral testicular torsion-detorsion

Cited by 57 publications

References 24 publications

An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

Antioxidant Systems and Oxidative Stress in the Testes

Dimethylsulfoxide attenuates ischemia-reperfusion injury in rat testis

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