An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

Kemahlı, Eray; Yildiz, Mevlüt; Fırat, Tülin; Özyalvaçlı, Mehmet Emin; Üyetürk, Uğur; Yılmaz, Burak; Gücük, Adnan

doi:10.5489/cuaj.3160

Cited by 11 publications

(10 citation statements)

References 24 publications

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“…Also, it has strong anti-inflammatory properties as it is a specific inhibitor of NFkB. 24,25,27 Thiol compounds such as N-acetyl-cysteine (NAC) or t-cysteine also inhibit NFkB activation. The basis of NFkB inhibition is the free oxygen radical scavenging effect of thiol groups.…”

Section: Discussionmentioning

confidence: 99%

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Protective effect of pyrrolidine dithiocarbamate against methotrexate-induced testicular damage

2021

Hum Exp Toxicol

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The aim of the study was to investigate the protective effect of pyrrolidine dithiocarbamate (PDTC) against methotrexate (MTX)-induced testicular damage in rats. Forty Wistar albino male rats were divided into equally four groups: Control group (saline solution, IP), PDTC group (100 mg/kg PDTC,IP, 10 days), MTX group (20 mg/kg MTX, IP, single dose, on the 6th day) and MTX + PDTC group (100 mg/kg PDTC, IP, 10 days and 20 mg/kg MTX, IP, single dose, on the 6th day). After 10 days, testicular tissues were excised for morphometric, histological and immunohistochemical evaluations. Serum testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH) and prokineticin 2 (PK2) levels were determined. Body and testicular weights were measured. Testicular damage was assessed by histological evaluation. Nuclear factor kappa B (NFkB), nuclear factor erythroid 2 related factor 2 (Nrf2) and PK2 immunoreactivities were evaluated by HSCORE. Body and testicular weights, serum FSH, LH, testosterone levels, seminiferous tubule diameter and germinal epithelial thickness were significantly decreased in the MTX group. However, serum PK2 level, histologically damaged seminiferous tubules and interstitial field width were significantly increased. Additionally, there was an increase in NFkB and PK2 immunoreactivity, whereas there was a significant decrease in Nrf2 immunoreactivity. PDTC significantly improved hormonal, morphometric, histological and immunohistochemical findings. Taken together, we conclude that PDTC may reduce MTX-induced testicular damage via NFkB, Nrf2 and PK2 signaling pathways.

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Section: Discussionmentioning

confidence: 99%

“…It is also a potent antioxidant. [24][25][26][27] Studies have reported that PDTC protects and improves histopathology by reducing oxidative stress in organ damage caused by various agents. 25,28,29 To the best of our knowledge, no study has examined the protective effect of PDTC on testicular damage caused by MTX.…”

Section: Introductionmentioning

confidence: 99%

Protective effect of pyrrolidine dithiocarbamate against methotrexate-induced testicular damage

2021

Hum Exp Toxicol

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“…Nuclear factor kappa Β plays a crucial role in immune response, cellular proliferation, inflammatory, and apoptosis[ 117 ]. Pyrrolidine dithiocarbamate (PDTC) is a stable low-molecular thiol compound which acts by neutralizing ROS[ 118 ]. Kemahli et al[ 118 ] studied the antioxidant effect of PDTC in a TT model and found that administration of PDTC exaggerates the antioxidant system by lowering MDA levels, increasing SOD activity and improving Johnson scores of biopsy specimen.…”

Section: Searchmentioning

confidence: 99%

Antioxidants in experimental ischemia-reperfusion injury of the testis: Where are we heading towards?

Vaos

Zavras

2017

WJM

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Testicular torsion (TT) is a medical emergency that primary affects newborns and young adolescents. It causes testicular injury due to the torsion of the spermatic cord and its components, initially in the venous blood flow and finally in the arterial blood flow. Prompt diagnosis and early surgical management are necessary in managing this urgent situation. The process of the pathophysiological events in ischemia-reperfusion is multifactorial and deals with the perception of the oxidative stress responsible for the consequences of ischemia/reperfusion (I/R) stress following TT. Duration and severity of torsion also play a significant role in the oxidative stress. A detrimental result of the defense system of the testes takes place resulting finally in testicular atrophy and impaired function. Antioxidant factors have been experimentally studied in an effort to front this state. They have been classified as endogenous or exogenous antioxidants. Endogenous antioxidants comprise a structure of enzymic enzymatic and non-enzymic enzymatic particles presented within cytoplasm and numerous other subunits in the cells. Exogenous antioxidants include a variety of natural and pharmaceutical agents that may prevent or ameliorate the harmful effects of I/R injury. In this study we review those factors and their ability to enhance the oxidative status of the testis. A feature insight into where we are heading is attempted.

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“…We used Johnsen’s score to evaluate the morphological damage of the seminiferous tubules [ 33 ]. When we checked the literature, we found that different research groups found significant decrease in Johnsen’s score of the testicular IR injury group compared to the control group [ 43 , 44 , 45 , 46 ]. In our study, there was a significant decrease of Johnsen’s score in IR group compared to Sham group as in other studies.…”

Section: ⧉ Discussionmentioning

confidence: 99%

Carvacrol treatment opens Kir6.2 ATP-dependent potassium channels and prevents apoptosis on rat testis following ischemia–reperfusion injury model

Balcı

Fırat²,

Acar³

et al. 2021

Rom J Morphol Embryol

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Testicular torsion is a urological problem that causes subfertility and testicular damage in males. Testis torsion and detorsion lead to ischemiareperfusion (IR) injury in the testis. Testicular IR injury causes the increase of reactive oxygen species (ROS), oxidative stress (OS) and germ cell-specific apoptosis. In this study, we aimed to investigate whether Carvacrol has a protective effect on testicular IR injury and its effects on Kir6.2 channels, which is a member of adenosine triphosphate (ATP)-dependent potassium channels. In the study, 2-4 months old 36 albino Wistar rats were used. For experimental testicular IR model, the left testis was rotated counterclockwise at 720º for two hours, and after two hours following torsion, detorsion was performed. Carvacrol was dissolved in 5% Dimethyl Sulfoxide (DMSO) at a dose of 73 mg/kg and half an hour before detorsion, 0.2 mL was administered intraperitoneally. In testicular tissues, caspase 3 and Kir6.2 immunoexpressions were examined. Serum malondialdehyde (MDA) and testosterone levels were measured. Apoptotic cells and serum MDA levels were significantly decreased and Kir6.2 activation was significantly increased in Carvacrol-administrated IR group. As a result of our study, Carvacrol may activates Kir6.2 channels and inhibits apoptosis and may have a protective effect on testicular IR injury.

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An experimental study on effects of pyrrolidine dithiocarbamate on ischemia-reperfusion injury in testis

Cited by 11 publications

References 24 publications

Protective effect of pyrrolidine dithiocarbamate against methotrexate-induced testicular damage

Protective effect of pyrrolidine dithiocarbamate against methotrexate-induced testicular damage

Antioxidants in experimental ischemia-reperfusion injury of the testis: Where are we heading towards?

Carvacrol treatment opens Kir6.2 ATP-dependent potassium channels and prevents apoptosis on rat testis following ischemia–reperfusion injury model

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