The Effect of Vincristine on Haemoglobin Synthesis

Ben‐Bassat, Isaac; Mozel, Miriam; Mark, Z.; Ramot, Bracha

doi:10.1111/j.1600-0609.1977.tb02110.x

Cited by 3 publications

(1 citation statement)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Apo ␣-globin is particularly unstable, as evidenced by its reduced secondary structure in solution (68). Moreover, ␣-globin precipitation is relatively increased in iron deficiency (69). AHSP binds newly synthesized apo ␣-globin to enhance its folding and solubility (22).…”

Section: Discussionmentioning

confidence: 99%

An Iron Responsive Element-like Stem-Loop Regulates α-Hemoglobin-stabilizing Protein mRNA

Santos

Doré

Valentine

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

Hemoglobin production during erythropoiesis is mechanistically coupled to the acquisition and metabolism of iron. We discovered that iron regulates the expression of ␣-hemoglobinstabilizing protein (AHSP), a molecular chaperone that binds and stabilizes free ␣-globin during hemoglobin synthesis. In primates, the 3-untranslated region (UTR) of AHSP mRNA contains a nucleotide sequence resembling iron responsive elements (IREs), stem-loop structures that regulate gene expression post-transcriptionally by binding iron regulatory proteins (IRPs). The AHSP IRE-like stem-loop deviates from classical consensus sequences and binds IRPs poorly in electrophoretic mobility shift assays. However, in cytoplasmic extracts, AHSP mRNA co-immunoprecipitates with IRPs in a fashion that is dependent on the stem-loop structure and inhibited by iron. Moreover, this interaction enhances AHSP mRNA stability in erythroid and heterologous cells. Our findings demonstrate that IRPs can regulate mRNA expression through non-canonical IREs and extend the repertoire of known iron-regulated genes. In addition, we illustrate a new mechanism through which hemoglobin may be modulated according to iron status.

show abstract

Section: Discussionmentioning

confidence: 99%

An Iron Responsive Element-like Stem-Loop Regulates α-Hemoglobin-stabilizing Protein mRNA

Santos

Doré

Valentine

et al. 2008

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

Desacetyl vinblastine amide sulfate induced ineffective erythropoiesis

Jumean

Camitta

Holcenberg

et al. 1979

Cancer

View full text Add to dashboard Cite

Ineffective erythropoiesis occurred during desacetyl vinblastine amide sulfate (VDS) therapy of a patient with metastatic Ewing's sarcoma. The peripheral blood was characterized by anisocytosis, poikilocytosis and reticulocytopenia. Bone marrow showed megaloblastic red cell hyperplasia with nuclear fragmentation, binuclearity and abnormal metaphases. Radioiron incorporation into red cells was markedly decreased. Electron microscopy showed red cell surface changes previously called ropalocytosis. Normal erythropoiesis promptly resumed when VDS treatment was withheld. Although less common than leukopenia, red cell abnormalities are part of the spectrum of vinca alkaloid toxicity.

show abstract