The Effect of Variation in Ionic Strength on the Apparent First and Second Dissociation Constants of Carbonic Acid

Hastings, A. Baird; Sendroy, Julius

doi:10.1016/s0021-9258(18)84852-9

Cited by 166 publications

(17 citation statements)

References 10 publications

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“…Calculated pH values for these experiments were pH 7.4 and pH 7.1. However, empirical pH values for these NaHCO 3 solutions equilibrated with 5% CO 2 were 7.58 (22 mM NaHCO 3 ) and 7.21 (11 mM HCO 3 ), which may be attributed to the increased pKa of HCO 3 − (Hastings & Sendroy, 1925) and decreased CO 2 solubility (Van Slyke et al 1928) (pH 6.6). These solutions approximate the ionic activity of native human ASL cultured at the air-liquid interface (Knowles et al 1997;Jayaraman et al 2001b;Namkung et al 2009).…”

Section: Solutionsmentioning

confidence: 98%

Paracellular bicarbonate flux across human cystic fibrosis airway epithelia tempers changes in airway surface liquid pH

Thornell

Rehman

Pezzulo

et al. 2020

The Journal of Physiology

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Cl − and HCO 3 − had similar paracellular permeabilities in human airway epithelia. r P Cl /P Na of airway epithelia was unaltered by pH 7.4 vs. pH 6.0 solutions. r Under basal conditions, calculated paracellular HCO 3 − flux was secretory. r Cytokines that increased airway surface liquid pH decreased or reversed paracellular HCO 3 − flux. r HCO 3 − flux through the paracellular pathway may counterbalance effects of cellular H + and HCO 3 − secretion.

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Section: Solutionsmentioning

confidence: 98%

Paracellular bicarbonate flux across human cystic fibrosis airway epithelia tempers changes in airway surface liquid pH

Thornell

Rehman

Pezzulo

et al. 2020

The Journal of Physiology

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“…The boundary CO 3--concentrations can be calculated from the boundary H+ and HCO 3-concentrations according to the mass action law. For this purpose the dissociation constant K 2 , is calculated from the thermodynamic constant K2a (see Landolt-B6rnstein, 1960) and the effect of the ionic strength (see Hastings and Sendroy, 1925) to be 1.1-10-1 mol/liter for the albumin solution of pH = 7.2 and 1.3. 10-'1 mol/liter for the solution of pH = 8.0.…”

Section: Role Of Carbonate Diffusionmentioning

confidence: 99%

Facilitated Diffusion of CO2 across Albumin Solutions

Gros

Moll

1974

The Journal of General Physiology

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The steady-state CO2 flux across thin layers of 30 g/100 ml albumin solutions was measured in two different C02 partial pressure ranges (boundary Pco 2 values 3 and 8 torr, and 160 and 650 torr, respectively). From the data the apparent diffusion coefficient for CO2, Dco 2 , was calculated. In the high Pco 2 range a value of Dco 2 was found which is to be expected on the basis of diffusion of dissolved CO2 only. In the low Pco 2 range Dco 2 was about 100 % higher than in the high Pco 2 range, when carbonic anhydrase was present and the pH was -7.7. Dco 2 depended on the concentration of carbonic anhydrase. It increased with increasing pH. It is concluded that an additional diffusion of bound CO 2 (facilitated CO2 diffusion) occurs in the low Pco 2 range and that this transport involves the hydration of CO2. From the diffusion coefficients in the two Pco 2 ranges the rate of facilitated diffusion was determined. Approximate calculations show that this rate (at pH < 7.7) can be explained on the basis of the proposed mechanism of facilitated CO 2 diffusion: bicarbonate diffusion and simultaneous proton transport by albumin diffusion. The view that facilitated CO 2 diffusion is mediated by the diffusion of albumin is supported by the experimental finding of a considerable suppression of the facilitated CO2 flux in the presence of gelatinized agar-agar.

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“…1. In a bicarbonate-carbon dioxide buffer system of pH 6.74, therefore, approximately 95 per cent of the previously formed * Using the dissociation constant for carbon dioxide and bicarbonate in saline as calculated by~lHastings and Sendroy (11). choline phosphoric acid would immediately react with bicarbonate ion, to liberate carbon dioxide into the gas phase.…”

Section: Possibilities Of Retocion E~ects (Cf Warburg (12) )--(A)mentioning

confidence: 99%

A Manometric Method for Measuring the Activity of the Cl. Welchii Lecithinase and a Description of Certain Properties of This Enzyme

Zamecnik

Brewster

Lipmann

1947

Journal of Experimental Medicine

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The Effect of Variation in Ionic Strength on the Apparent First and Second Dissociation Constants of Carbonic Acid

Cited by 166 publications

References 10 publications

Paracellular bicarbonate flux across human cystic fibrosis airway epithelia tempers changes in airway surface liquid pH

Paracellular bicarbonate flux across human cystic fibrosis airway epithelia tempers changes in airway surface liquid pH

Facilitated Diffusion of CO2 across Albumin Solutions

A Manometric Method for Measuring the Activity of the Cl. Welchii Lecithinase and a Description of Certain Properties of This Enzyme

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