A manometric method has been described for measurement of the activity of Cl. welchii lecithinase. The method depends on displacement of carbon dioxide from a bicarbonate-carbon dioxide-buffered medium by the phosphorylcholine liberated during the enzymatic hydrolysis.
LIPIDS AND CZ. welchii TOXIN 33 time we added amboceptor and erythrocytes. After 1 0 minutes on the waterbath we read the reaction and controlled this after 20 minutes at room temperature. The complementfixation reaction was also done at ice-box temperature. Our impression was that the reaction proceeded more rapidly and in higher titre. Otherwise our results were essentially the same. We compared these results with those obtained with an antigen prepared exactly after Craig's method. The 2 antigens gave in our hands identical results. A total of 121 sera were tested with the 2 antigens. In only 5 of these patients could we demonstrate E. histolytica in the feces. The serological reaction was positive in only one of the healthy amebic carriers as well as in one patient who showed no ameba in the feces. This latter patient had an uncertain liver disturbance, but our numerous fecal examinations remained negative for ameba. Our sole purpose for presenting these data is to induce investigators having access to greater clinical material, to repeat the serological reaction in amebic dysentery with the saline suspension antigen of E . histolytka. Summary. A saline suspension of E. histolytica was employed as antigen in the complement-fixation test which gave identical results with those obtained with the Craig alcoholic extract antigen of E . h2stolytica.
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