The effect of using PPM (plant preservative mixture) on the development of cauliflower microshoots and the quality of artificial seed produced

Effect of Different Sterilization Methods on Contamination and Viability of Nodal Segments of Cestrum nocturnumL

“…More experiments carried out using PPM as an addition to the medium. [12] showed that using the PPM can effectively control contamination in the cauliflower micro-shoot micropropagation system. The use of 0.5 ml/l PPM added to the culture medium and 1 ml/l PPM in the blending medium found to be optimal for controlling the contamination without causing a reduction in the effectiveness of the culture system.…”

Artificial Seeds (Principle, Aspects and Applications)

“…These consequences prove the ability of such explants for encapsulation and artificial seed production. The encapsulation of cauliflower microshoots for artificial seed production was successfully achieved and the capacity to convert in commercial substrates was confirmed as well [11,76].…”

“…However, although the design of such systems was achieved in some plant species, such as cauliflower (Brassica oleraceae var. botrytis L.) [11,76,[96][97][98], the micropropagation system is still one of the major limitations of the development of artificial seed technology.…”

“…However, this pathway encountered some complications such as the difficulties of achieving one rooting step for non-embryogenic artificial seeds [101]. The difficulties of sowing artificial seeds directly in soil or in commercial substrates such as compost, vermiculite, etc., under non-sterile conditions are considered to be one of the main limitations of the practical use of this technique [76,102].…”

Effect of Various Surface Sterilant on Contamination and Callus Regeneration of Ashoka (Saraca asoca Roxb. De Wilde) from Leaf Segment Explant

“…The sterilizing agent should be used for an appropriate duration to control contamination. However HgCl 2 which has mainly antibacterial action was more efficient and should be used for decontamination percentage ( Rihan , 2012) The data presented in table 2. revealed that explants treated with 0.1% HgCl 2 for 1 minute significantly reduced the days to callus initiation(37.67) remaining at par with T 4 (0.1% HgCl 2 for 3 minutes),T 5 (0.1% HgCl 2 for 4 minutes), T 6 (0.1% HgCl 2 for 5 minutes) and T 7 (0.1% HgCl 2 for 6 minutes) and callusing percentage were significantly higher (93.33 %) in T 19 (0.1% HgCl 2 for 15 minutes +1% NaOCl for 2 Min) remaining at par with T 12 (0.1% HgCl 2 for 15 minutes). The callus spread was higher (2.33 × 1.00) in T 19 (0.1% HgCl 2 for 15 minutes +1% NaOCl 2 for 2 Min).…”