The Effect of Type and Dose of Anti-Inflammatory Drugs on the Viability and Osteogenic Potential of Dental Pulp Stem Cells

Abstract

Objectives: The use of nonsteroidal anti-inflammatory drugs (NSAIDs) after regenerative surgeries may affect the behavior of stem cells used during the treatment. This study investigates the possible effect of NSAIDs on the proliferation and the osteo-differentiation of dental pulp stem cells (DPSCs) in vitro. Methods : DPSCs were isolated, cultured and characterized with human antibodies CD90, CD105 and CD45. Cells were cultured for a period of 1, 3 and 5 days to assess the viability using different drug concentrations of acetylsalicylic acid (ASA) 10, 50, and 200 μg/mL., Diclofenac sodium 10 -4 and 10 -6 M and Meloxicam 0.01, 0.1, and 1 μM .Selected concentration of ASA 10 μg/ mL, Diclofenac sodium 10 -6 M and Meloxicam 0.1 μM were used during osteo-differentiation of the cells.Results : DPSCs viability assay recorded the highest absorption rate on the 1 st day with ASA 10 μg/mL and on the 3 rd day with Meloxicam 0.01 μM. On day 5 all concentration of ASA and Diclofenac reported the highest absorption rats. The highest values of calcium compounds was expressed with ASA 10 μg/mL on the 7 th day using alizarin red satin. Conclusion :Using different types of NSAIDs during the postsurgical regimen can affect the viability and osteo-differentiation of DPSCs.