Our findings suggest that bone marrow, adipose tissue and dental pulp may serve as a universal donor MSC source for the prevention of burn wound progression.

Objective The aim of our study was to investigate the effect of Transforming growth factor beta-1 (TGF- β 1) gene therapy on the surface markers, multilineage differentiation, viability, apoptosis, cell cycle, DNA damage and senescence of human Dental Pulp-derived Mesenchymal Stromal Cells (hDPSC). Methods hDPSCs were isolated from human teeth, and were cultured with 20% Fetal Bovine Serum (FBS) in minimum essential media-alpha ( α -MEM). TGF- β 1 gene transfer into hDPSCs was performed by electroporation method after the plasmid was prepared. The transfection efficiency was achieved by using western blot and flow cytometry analyses and GFP transfection. Mesenchymal stem cell (MSC) markers, multilineage differentiation, cell proliferation, apoptosis, cell cycle, DNA damage and cellular senescence assays were performed by comparing the transfected and non-transfected cells. Statistical analyses were performed using GraphPad Prism. Results Strong expression of TGF- β 1 in pCMV-TGF- β 1-transfected hDPSCs was detected in flow cytometry analysis. TGF- β 1 transfection efficiency was measured as 95%. Western blot analysis showed that TGF- β 1 protein levels increased at third and sixth days in pCMV-TGF- β 1-transfected hDPSCs. The continuous TGF- β 1 overexpression in hDPSCs did not influence the immunophenotype and surface marker expression of MSCs. Our results showed that TGF- β 1 increased osteogenic and chondrogenic differentiation, but decreased adipogenic differentiation. Overexpression of TGF- β 1 increased the proliferation rate and decreased total apoptosis in hDPSCs (p<0.05). The number of cells at “ S ” phase was higher with TGF- β 1 transfection (p<0.05). Cellular senescence decreased in TGF- β 1 transfected group (p<0.05). Conclusions These results reflect that TGF- β 1 has major impact on MSC differentiation. TGF- β 1 transfection has positive effect on proliferation, cell cycle, and prevents cellular senescence and apoptosis.

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Purpose: The aim of this study is to compare effects of postoperative care agents; chlorhexidine, octenidine dihydrochloride and hyaluronic acid on human gingival fibroblasts’ viability, proliferation, apoptosis and migration. Materials and methods: After cell culturing; chlorhexidine, octenidine dihydrochloride and hyaluronic acid solutions were applied on cells and nothing was applied for control group. The cells were monitored to investigate cytotoxicity; the percentage of apoptotic, living and dead cells at the time of 24, 48, and 72 hours (h). A scratch wound assay was performed to detect cell migration and cells were monitored at baseline, at 24 and 48h. Results: At 24h, chlorhexidine showed statistically lower percentage of total apoptotic cells’ than octenidine dihydrochloride (p=0.049), hyaluronic acid (p=0.049) and control (p=0.049). At 48h, hyaluronic acid showed statistically lower percentage than chlorhexidine (p=0.049), and control (p=0.049). All agents were found to have statistically and significantly more cytotoxic than control. However, there was no difference between experimental groups for proliferation rate. Octenidine dihydrochloride showed statistically negative effects on cell migration than chlorhexidine and hyaluronic acid at 24h. Chlorhexidine and hyaluronic acid maintained migration ability of cells than octenidine dihydrochloride at 48h. Conclusion: All agents have similar effects on cell behavior such as viability, apoptosis and cell proliferation. However, octenidine dihydrochloride showed statistically negative effects on migration ability than chlorhexidine and hyaluronic acid.

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