Effects of non-steroidal anti-inflammatory drug (ibuprofen) in low and high dose on stemness and biological characteristics of human dental pulp-derived mesenchymal stem cells

Salkın, Hasan; Basaran, Kemal Erdem

doi:10.1080/03008207.2022.2083613

Cited by 5 publications

(4 citation statements)

References 35 publications

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“…Meanwhile, doses of ibuprofen used in the study of Salkin et al (2022) were several times higher than therapeutic plasma concentration. In their research, high-dose ibuprofen (3mmol/l) significantly increased CD73 expression in DPSCs [35], which indicates dosagedependent results.…”

Section: Discussionmentioning

confidence: 90%

“…Regarding cellular viability, there is conflicting evidence in the literature in the case of NSAIDS effect on stem cell viability. While doses of ibuprofen used in the study by Salkin et al (2022) were 0.1mmol/l and 3mmol/l, DPSCs viability increased significantly in the ibuprofen-applied groups [35]. However, different results were revealed with therapeutic concentrations of ibuprofen pre-treatment (25μg/ml, 50μg/ml) on human bone marrow mesenchymal stem cells.…”

Section: Discussionmentioning

confidence: 93%

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Effects of Ibuprofen and Diclofenac Pre-treatment on Viability and Apoptosis Processes in Human Dental Pulp Stem Cells

Adamickova,

Gažová,

Adamicka

et al. 2024

Preprint

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Stem cell-based regeneration strategies have shown therapeutic efficacy in various fields of regenerative medicine. These include bone healing after bone augmentation, often complicated by pain, that is managed by using non-steroidal anti-inflammatory drugs (NSAIDSs). Information is limited about how NSAIDSs affect the therapeutic potential of stem cells. Therefore, we investigated the effects of ibuprofen and diclofenac in low and high therapeutic doses on the properties of human mesenchymal stromal cells isolated from the dental pulp (DPSCs) and cultured in vitro. Ibuprofen and diclofenac significantly reduced the viability of DPSCs, while the expression of mesenchymal stem cell surface markers was unaffected. Both ibuprofen and diclofenac treatment significantly upregulated the expression of HGF, while the expression of VEGFA remained unchanged. Ibuprofen significantly altered the expression of several apoptosis-related genes, including upregulation of CASP9 and BCL2, with decreased CASP3 expression. BAK, CASP3, CASP9, and BCL2 expressions were significantly increased in the diclofenac-treated DPSCs, while no difference was demonstrated in BAX expression. Our results suggest that concomitant use of the NSAIDSs ibuprofen or diclofenac with stem cell therapy may negatively impact cell viability and alter the expression of apoptosis-related genes, which can affect the efficacy of stem cell therapy.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 93%

Effects of Ibuprofen and Diclofenac Pre-treatment on Viability and Apoptosis Processes in Human Dental Pulp Stem Cells

Adamickova,

Gažová,

Adamicka

et al. 2024

Preprint

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show abstract

“…The improvement in the stemness characteristics of DPSCs related to the use of ibuprofen or other NSAIDs, according to the authors, could be useful in the treatment of inflammatory diseases such as osteoarthritis (OA). They speculate that in OA treatment ibuprofen may have similar effects as curcumin [ 13 ]; in fact, curcumin anti-inflammatory action has been demonstrated to inhibit the activation of COX-2 involved in OA processes [ 110 ]. However, further studies are needed to more closely examine the mechanisms of ibuprofen on DPSCs.…”

Section: Non-natural Compoundsmentioning

confidence: 99%

“…In addition, non-natural molecules have shown similar beneficial effects on cell differentiation of bone tissues [ 13 ]. Furthermore, the combined use of stem cell therapy and such natural or pharmacological sources can enhance the osteogenic differentiation capacity of MSCs.…”

Section: Introductionmentioning

confidence: 99%

Molecules Inducing Dental Stem Cells Differentiation and Bone Regeneration: State of the Art

Ariano

Posa

Storlino

et al. 2023

IJMS

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Teeth include mesenchymal stem cells (MSCs), which are multipotent cells that promote tooth growth and repair. Dental tissues, specifically the dental pulp and the dental bud, constitute a relevant source of multipotent stem cells, known as dental-derived stem cells (d-DSCs): dental pulp stem cells (DPSCs) and dental bud stem cells (DBSCs). Cell treatment with bone-associated factors and stimulation with small molecule compounds are, among the available methods, the ones who show excellent advantages promoting stem cell differentiation and osteogenesis. Recently, attention has been paid to studies on natural and non-natural compounds. Many fruits, vegetables, and some drugs contain molecules that can enhance MSC osteogenic differentiation and therefore bone formation. The purpose of this review is to examine research work over the past 10 years that has investigated two different types of MSCs from dental tissues that are attractive targets for bone tissue engineering: DPSCs and DBSCs. The reconstruction of bone defects, in fact, is still a challenge and therefore more research is needed; the articles reviewed are meant to identify compounds useful to stimulate d-DSC proliferation and osteogenic differentiation. We only consider the results of the research which is encouraging, assuming that the mentioned compounds are of some importance for bone regeneration.

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Regulation Mechanisms and Maintenance Strategies of Stemness in Mesenchymal Stem Cells

Jiang,

Tian,

Wang

et al. 2023

Stem Cell Rev and Rep

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Effects of non-steroidal anti-inflammatory drug (ibuprofen) in low and high dose on stemness and biological characteristics of human dental pulp-derived mesenchymal stem cells

Cited by 5 publications

References 35 publications

Effects of Ibuprofen and Diclofenac Pre-treatment on Viability and Apoptosis Processes in Human Dental Pulp Stem Cells

Effects of Ibuprofen and Diclofenac Pre-treatment on Viability and Apoptosis Processes in Human Dental Pulp Stem Cells

Molecules Inducing Dental Stem Cells Differentiation and Bone Regeneration: State of the Art

Regulation Mechanisms and Maintenance Strategies of Stemness in Mesenchymal Stem Cells

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