The Effect of Tobramycin on Rabbit Eyes

Purnell, W. David; McPherson, Samuel D.

doi:10.1016/0002-9394(74)90474-7

Cited by 15 publications

(3 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, netilmicin ophthalmic solution (NETTACIN eye drops 0.3%), is also formulated and available in some countries as a preservative-free solution, thus lacking the detrimental effects of preservatives on the ocular surface (16)(17)(18)(19)(20). However, netilmicin, as with all other aminoglycosides (33)(34)(35)(36), does not easily penetrate the corneal epithelial barrier because of its hydrophilic properties and, consequently, effective concentrations of the drugs cannot be attained in the anterior chamber by topical administration. For all these reasons, netilmicin may be considered as an "ideal" antibiotic for the treatment of external eye disease; however, its influence on epithelial proliferation and integrity is at present unknown.…”

Section: Discussionmentioning

confidence: 96%

Effect of Ofloxacin and Netilmicin on Human Corneal and Conjunctival CellsIn Vitro

Papa¹,

Leonardi

Getuli³

et al. 2003

Journal of Ocular Pharmacology and Therapeutics

View full text Add to dashboard Cite

The purpose of this study was to compare the cytotoxic effects of the fluoroquinolone ofloxacin with that of the aminoglycoside netilmicin. Human corneal epithelial cells (HCE-T) and human conjunctival epithelial cells (Wong-Kilbourne derivative of Chang conjunctiva) were exposed to antibiotics (0.08-5.0 mg/mL) for 4 or 24 hours. Cell proliferation and viability were assessed with the MTT assay, neutral red uptake, and bromo deoxy uridine incorporation. In both cell lines, ofloxacin inhibited cell proliferation and viability. These effects were time and dose dependent. Concentrations of ofloxacin ranging from 0.4 to 2.4 mg/mL (0.04% to 0.24%) produced a 50% inhibition of proliferation and viability. In contrast, netilmicin induced no toxic effect. The differences between ofloxacin and netilmicin were highly statistically significant (p < 0.001). This finding is particularly relevant in deciding the optimal antibiotic to be applied in clinical situations in which the epithelium is compromised.

show abstract

Section: Discussionmentioning

confidence: 96%

Effect of Ofloxacin and Netilmicin on Human Corneal and Conjunctival CellsIn Vitro

Papa¹,

Leonardi

Getuli³

et al. 2003

Journal of Ocular Pharmacology and Therapeutics

View full text Add to dashboard Cite

show abstract

“…Subconjunctival aminoglycosides and other antibiotics have been known to penetrate into aqueous or corneal tissuell5ll9,120 [134][135][136] and reported to have a clinically therapeutic effect in experimental bacterial keratitis. 101,103,106,107,112 However, the quantitative microbiologic effect of subconjunctival aminoglycosides has been unclear, and their relative efficacy and value have been questioned, compared with fortified topical regimens. 110,119'122,123 Also, their role as therapy adjunctives to an intensive topical regimen has been unclear.…”

Section: Subconjunctival Therapymentioning

confidence: 99%

Experimental Pseudomonas Keratitis

Hyndiuk

Davis

Hatchell

et al. 1983

Cornea

View full text Add to dashboard Cite

“…aeruginosa (Purnell and McPherson, 1974), it rapidly penetrates the eye. And since it is now available as an injectable solution (Nebcin@*), we decided to test both its efficacy and its toxicity when used to treat Pseudomonas-infected rabbit eyes, and to compare these effects with those of gentamicin.…”

mentioning

confidence: 99%