The effect of the insecticide dichlorvos on esterase activity extracted from the psocids, Liposcelis bostrychophila and L. entomophila

Wang, Jinjun; Cheng, Wei-Xia; Ding, Wei; Zhao, Zuomin

doi:10.1093/jis/4.1.23

Cited by 36 publications

(13 citation statements)

References 21 publications

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“…These enzymes are playing a vital role in insect pests; especially, the esterase family is helped in digestion, reproduction and metabolism of juvenile hormone, metamorphosis and detoxification of toxic substances [12,13]. Among the various enzymes in esterase families, one of the enzyme called acetylcholinesterase is present at nerve synapses and it helps to transmission of nerve impulse by hydrolysis of acetylcholine [14]. Carboxylesterase, is another important enzyme that plays a vital role in degradation of neurotransmitter, metabolism of specific hormones and pheromones, consequently involved in insect development and behavior, detoxification of toxic substances etc.…”

Section: Introductionmentioning

confidence: 99%

Biocompatible green synthesized silver nanoparticles impact on insecticides resistant developing enzymes of dengue transmitted mosquito vector

et al. 2019

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The growing interest in nanomaterial-based products in various fields urged to study the influence of different nanoproducts on the ecosystem and on various insect pests. In this study, we evaluated insecticides resistant influenced important biochemical constituents of A. aegypti upon the exposure of green synthesized A. reticulata silver nanoparticle (Ar-AgNPs) for 24 h at their lethal threshold concentration. The AgNPs exposed and control mosquito larval homogenates of the protein content, acetylcholinesterase, specific detoxification enzymes α-and β-carboxylesterase and other anti-oxidant or effective insecticides resistant developed enzyme named glutathione S-transferase (GST) were studied. The larvicidal activity of AgNPs at their lethal threshold concentration (4.5 µg/ml) were monitored for 12 and 24 h and prompted time dependent mortality rate were 23.3 ± 0.57 and 46.6 ± 0.57% respectively. Further, the biochemical constituents of protein content of AgNPs exposed larval homogenates were quantitatively higher compared to the control, but, in the qualitative analysis of separated protein in the electrophoresed gel revealed, the expression protein was down regulated. The important esterase enzymes of acetylcholinesterase, α-and β-carboxylesterase and GST enzymes were significantly reduced upon the exposure of AgNPs compared to the control larval body homogenates respectively. In the qualitative analysis, isoenzyme of β-carboxylesterase expression level was also down regulated. Followed by these investigations, it is clearly showed that A. reticulata mediated synthesized AgNPs have the potent deleterious impact on fourth instar larvae of A. aegypti physiological system by inhibiting important esterase enzyme groups.

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Section: Introductionmentioning

confidence: 99%

Biocompatible green synthesized silver nanoparticles impact on insecticides resistant developing enzymes of dengue transmitted mosquito vector

et al. 2019

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“…The infection mechanism of Beauveria in the host insects has been reported (Roberts et al 2004; Wang et al 2004; Thomas et al 2007). The primary route of host invasion is through the external integument via the attachment of the conidia to the cuticle, germination, followed by penetration into the cuticle.…”

Section: Introductionmentioning

confidence: 99%

The Effect ofBeauveria brongniartiiand its Secondary Metabolites on the Detoxification Enzymes of the Pine Caterpillar,Dendrolimus tabulaeformis

Fan

Xie

Xue

et al. 2013

Journal of Insect Science

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The mortality of pine caterpillar, Dendrolimus tabulaeformis Tsai et Liu (Lepidoptera: Lasiocampidae), larvae treated with Beauveria brongniartii (Saccardo) Petch (Hypocreales: Clavicipitaceae) conidia and cell-free culture supernatants enriched for the secondary metabolites of the fungus was investigated. In addition, the effects of the treatments on the activities of two insect-related defense response proteins, glutathione S-transferase (GST) and esterase (EST), were measured over time. Bioassays were performed using a range of fungal spore (6 × 105 through 6 × 107 spores/mL) and supernatant extract concentrations (5.5–550 µg/mL). The results showed that the mortalities of D. tabulaeformis larvae were closely related to the concentration of the conidia and the metabolites of B. brongniartii. The differences among the treatments all reached a significant level. The activities of the two detoxifying enzymes, GST and EST, in the larvae increased simultaneously post-treatment. After infection with the conidial suspensions, the highest GST activity appeared at 3 days, and the activities of the caterpillars infected with 6 × 106 spores/mL and 6 × 107 spores/mL were significantly higher than in the control. Using α-naphthyl, the highest activity of EST also appeared at 3 days, and the differences for the three different concentrations were significant. A similar trend of change in the EST activity was observed using β-naphthyl. After treatment with the secondary metabolite solution, the highest GST activity appeared at 6 hr, and significant differences were found both for the different durations (2, 4, 6, 12, 24, and 48 hr) and in the three concentration groups. When using α-naphthyl, the EST activity peak appeared at 24 hr, and the differences were significant among the durations of 2, 4, 6, 12, 24, and 48 hr. The effect of the concentration of the secondary metabolite solution notably induced the EST activity in the insects, and a similar result was obtained using β-naphthyl. The data suggest that B. brongniartii produces secondary metabolites that disable the immune mechanisms of D. tabulaeformis, allowing the fungus to overcome and then kill its host. It was concluded that both the conidial suspensions and the metabolites of B. brongniartii were toxic to D. tabulaeformis larvae.

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“…Transcriptome studies showed that P450s, GSTs, and ESTs may play a role in insecticide tolerance after short-term exposure to deltamethrin and malathion (Dou et al, 2013). Also enhanced carboxylesterase activities may contribute to insecticide tolerance or resistance in booklice (Wang et al, 2004). Elevated GST activities were also confirmed in DDVP and PH 3 -resistant strains of L. bostrychohila by enzymatic assays (Dou et al, 2009).…”

Section: Introductionmentioning

confidence: 94%

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

et al. 2020

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Esterases (ESTs) play important roles in metabolizing various physiologically endogenous and exogenous compounds, and various environmental xenobiotics in insects. The psocid, Liposcelis bostrychophila is a major pest of stored products worldwide and rapidly develops resistance to commonly insecticides. However, the involvement of ESTs in insecticide metabolization and the application of RNAi approach in psocids have not been well elucidated. In this study, we characterized four LbEST genes and investigated the transcriptional levels of these genes at different developmental stages and under different insecticides exposures to assess their potential roles in response to insecticides. The four LbESTs contain a catalytic triad (Ser-His-Glu) linked to an oxyanion hole and acyl pocket involved in substrate stabilization during its hydrolysis. Synergism observed with the esterase-inhibitor DEF suggests the involvement of esterases in malathion detoxification. LbESTs were expressed during the whole of developmental stages, but predominant abundance in the first nymphal instar and adult stage. The mRNA level of three LbEST genes (except for LbEST4) was induced (1.29-to 5.60 fold) in response to malathion or deltamethrin exposures, indicating that these esterases are involved in the detoxification process. Silencing of LbEST1, LbEST2 or LbEST3 through dsRNA feeding led to a higher mortality of psocids upon the malathion treatment compared to controls (1.83 to 2.69-fold), demonstrating that these esterase genes play roles in malathion detoxification in L. bostrychophila. Our study provides new evidence for understanding of the function and regulation mechanism of esterases in L. bostrychophila in insecticide detoxification. The current study also suggests that the present RNAi method could be applied for gene functional studies in psocids.

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The effect of the insecticide dichlorvos on esterase activity extracted from the psocids, Liposcelis bostrychophila and L. entomophila

Cited by 36 publications

References 21 publications

Biocompatible green synthesized silver nanoparticles impact on insecticides resistant developing enzymes of dengue transmitted mosquito vector

Biocompatible green synthesized silver nanoparticles impact on insecticides resistant developing enzymes of dengue transmitted mosquito vector

The Effect ofBeauveria brongniartiiand its Secondary Metabolites on the Detoxification Enzymes of the Pine Caterpillar,Dendrolimus tabulaeformis

Characterization of Esterase Genes Involving Malathion Detoxification and Establishment of an RNA Interference Method in Liposcelis bostrychophila

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