Biocompatible green synthesized silver nanoparticles impact on insecticides resistant developing enzymes of dengue transmitted mosquito vector

Abstract

The growing interest in nanomaterial-based products in various fields urged to study the influence of different nanoproducts on the ecosystem and on various insect pests. In this study, we evaluated insecticides resistant influenced important biochemical constituents of A. aegypti upon the exposure of green synthesized A. reticulata silver nanoparticle (Ar-AgNPs) for 24 h at their lethal threshold concentration. The AgNPs exposed and control mosquito larval homogenates of the protein content, acetylcholinesterase, specific detoxification enzymes α-and β-carboxylesterase and other anti-oxidant or effective insecticides resistant developed enzyme named glutathione S-transferase (GST) were studied. The larvicidal activity of AgNPs at their lethal threshold concentration (4.5 µg/ml) were monitored for 12 and 24 h and prompted time dependent mortality rate were 23.3 ± 0.57 and 46.6 ± 0.57% respectively. Further, the biochemical constituents of protein content of AgNPs exposed larval homogenates were quantitatively higher compared to the control, but, in the qualitative analysis of separated protein in the electrophoresed gel revealed, the expression protein was down regulated. The important esterase enzymes of acetylcholinesterase, α-and β-carboxylesterase and GST enzymes were significantly reduced upon the exposure of AgNPs compared to the control larval body homogenates respectively. In the qualitative analysis, isoenzyme of β-carboxylesterase expression level was also down regulated. Followed by these investigations, it is clearly showed that A. reticulata mediated synthesized AgNPs have the potent deleterious impact on fourth instar larvae of A. aegypti physiological system by inhibiting important esterase enzyme groups.

Synthesis of Green Engineered Silver Nanoparticles through Urtica dioica: An Inhibition of Microbes and Alleviation of Cellular and Organismal Toxicity in Drosophila melanogaster

“…The AChE levels in the UD-AgNPs were substantially greater than those in the other groups. AChE levels were found to be increased by green synthesized NPs and AChE inhibition in AgNO 3 -exposed organisms, which is consistent with earlier findings [ 74 , 75 ].…”

Synthesis of MnCoO/CNT nanoflakes for the photocatalytic degradation of methyl orange dye and the evaluation of their activity against Culex pipiens larvae in the purification of fresh water

“…Moreover, protein has a main role in the insects during metamorphosis involving epidermal formation and synthesis of chitin, but when insects are exposed to the insecticide, protein level changes in the whole body can be triggered by down-regulation or up-regulation. 49,50 As summarized in Table 2, the level of acid phosphatase activity drastically decreased from 1041.61 of control larvae to 504.72 and 555.36 U g −1 upon exposure to MnCoO and MnCoO/CNTs, respectively. Additionally, the value of alkaline phosphatase declined from 2113.37 for control to 74.86 and 200.25 U g −1 in the 4th larval instar of C. pipiens treated with MnCoO and MnCoO/CNTs, respectively for an equal length of time.…”