The effect of surface treatment of titanium with sand-blasting/acid-etching or hydroxyapatite-coating and application of bone morphogenetic protein-2 on attachment, proliferation, and differentiation of stem cells derived from buccal fat pad

Park, Jun‐Beom; Kim, Youn Sun; G, Lee; Yun, Byeong Gon; Kim, Chang-Hyen

doi:10.1007/s13770-013-0011-6

Cited by 16 publications

(18 citation statements)

References 28 publications

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“…Gingiva from the maxillofacial region may be considered as a favorable source of mesenchymal stem cells as the harvesting of stem cells from the mandible or maxilla can be performed easily under local anesthesia (19). Furthermore, harvesting stem cells from the gingiva may be more practical when compared with the bone marrow of the maxilla and mandible as it is less invasive and has less complications, including paresthesia and pain (20)(21)(22).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

Jeong

Kim

Lee

et al. 2012

Molecular Medicine Reports

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Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

Jeong

Kim

Lee

et al. 2012

Molecular Medicine Reports

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“…Therefore, single-material-based scaffolds would be inefficient in maintaining the strength and rigidity which are normally required. Moreover, it is utmost important to find out materials which possess bone conductive properties and exactly simulate the ECM of bone [7–9]. An ideal tissue engineering scaffold is the one which can exactly mimic the extracellular matrix environment [10].…”

Section: Introductionmentioning

confidence: 99%

Development of Novel Biocomposite Scaffold of Chitosan-Gelatin/Nanohydroxyapatite for Potential Bone Tissue Engineering Applications

et al. 2016

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In this study, a three-dimensional chitosan-gelatin/nanohydroxyapatite (ChG/nHaP) scaffold was successfully fabricated and characterized in terms of swelling, degradation, cell proliferation, cell attachment, and mineralization characterizations. The ChG/nHaP scaffold was fabricated with a mean pore size of 100–180 μm. Our results showed that the physicochemical and biological properties of the scaffolds were affected by the presence of HaP. The swelling and degradation characteristics of the ChG scaffold were remarkably decreased by the addition of HaP. On the other hand, the presence of HaP remarkably improved the MC3T3-E1 cell attachment and cell growth in the scaffold membrane. The biocompatible nature of the ChG/nHaP scaffold leads to the development of finely scaled mineral deposits on the scaffold membrane. Thus, HaP played an important role in improving the biological performance of the scaffold. Therefore, the ChG/nHaP scaffold could be applied as a suitable material for bone tissue engineering applications.

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“…Gingiva-derived stem cells from the maxillofacial region may be considered a favorable source of mesenchymal stem cells as harvesting stem cells from the mandible or maxilla may be easily performed under local anesthesia (8,10). The present study was performed to produce stem-cell spheroids using concave microwells and to evaluate the maintenance of stemness, viability, and differentiation potential.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the maintenance of stemness, viability, and differentiation potential of gingiva-derived stem-cell spheroids

Lee¹,

Ko²,

Park³

2017

Experimental and Therapeutic Medicine

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Gingiva-derived stem cells have been applied for tissue-engineering purposes and may be considered a favorable source of mesenchymal stem cells as harvesting stem cells from the mandible or maxilla may be performed with ease under local anesthesia. The present study was performed to fabricate stem-cell spheroids using concave microwells and to evaluate the maintenance of stemness, viability, and differentiation potential. Gingiva-derived stem cells were isolated, and the stem cells of 4×105 (group A) or 8×105 (group B) cells were seeded into polydimethylsiloxane-based, concave micromolds with 600 µm diameters. The morphology of the microspheres and the change of the diameters of the spheroids were evaluated. The viability of spheroids was qualitatively analyzed via Live/Dead kit assay. A cell viability analysis was performed on days 1, 3, 6, and 12 with Cell Counting Kit-8. The maintenance of stemness was evaluated with immunocytochemical staining using SSEA-4, TRA-1-60(R) (positive markers), and SSEA-1 (negative marker). Osteogenic, adipogenic, and chondrogenic differentiation potential was evaluated by incubating spheroids in osteogenic, adipogenic and chondrogenic induction medium, respectively. The gingiva-derived stem cells formed spheroids in the concave microwells. The diameters of the spheroids were larger in group A than in group B. The majority of cells in the spheroids emitted green fluorescence, indicating the presence of live cells at day 6. At day 12, the majority of cells in the spheroids emitted green fluorescence, and a small portion of red fluorescence was also noted, which indicated the presence of dead cells. The spheroids were positive for the stem-cell markers SSEA-4 and TRA-1-60(R) and were negative for SSEA-1, suggesting that these spheroids primarily contained undifferentiated human stem cells. Osteogenic, adipogenic, and chondrogenic differentiation was more evident with an increase of incubation time: Mineralized extracellular deposits were observed following Alizarin Red S staining at days 14 and 21; oil globules were increased at day 18 when compared with day 6; and Alcian blue staining was more evident at day 18 when compared with day 6. Within the limits of this study, stem-cell spheroids from gingival cells maintained the stemness, viability, and differentiation potential during the experimental periods. This method may be applied for a promising strategy for stem-cell therapy.

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The effect of surface treatment of titanium with sand-blasting/acid-etching or hydroxyapatite-coating and application of bone morphogenetic protein-2 on attachment, proliferation, and differentiation of stem cells derived from buccal fat pad

Cited by 16 publications

References 28 publications

Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

Development of Novel Biocomposite Scaffold of Chitosan-Gelatin/Nanohydroxyapatite for Potential Bone Tissue Engineering Applications

Evaluation of the maintenance of stemness, viability, and differentiation potential of gingiva-derived stem-cell spheroids

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