Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

Jeong, Seong Mok; Kim, Bo‐Bae; Lee, Ji‐Eun; Ko, Youngkyung; Park, Jun‐Beom

doi:10.3892/mmr.2015.3456

Cited by 20 publications

(14 citation statements)

References 19 publications

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“…Gingiva can be easily accessible and obtainable [10,[21][22][23][24][25]. This procedure can be performed under local anesthesia and the obtained tissues are usually discarded in routine periodontal surgery [11,[26][27][28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of Bambusa tulda on osteogenic differentiation and mineralization of human stem cells

Lee¹,

Uddin²,

Lee³

et al. 2018

biomedicalresearch

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Bambusa tulda has been used for various Purpose and is considerd as one of the most useful bamboo species. This study aimed to evaluate the effects of Bambusa tulda extract (BBT) on the osteogenic differentiation and mineralization of human mesenchymal stem cells. Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of BBT at concentrations ranging from 0.001% to 1%. Evaluations of cell morphology and cellular viability were done at days 1, 3, 5 and 7. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells. The morphology of stem cells in the presence of BBT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not show any noticeable changes when compared with the untreated control group. The treatment of BBT (from 0.001% to 1% groups) showed decrease in alkaline phosphatase activity. The results of the Alizarin Red S staining showed a significant decrease with application of BBT. Conclusively, Bambusa tulda had influenced the osteogenic differentiation of the stem cells derived from the gingiva. Thus, the use of Bambusa tulda may produce adverse effects onto oral tissues. The concentration and application time of Bambusa tulda should be meticulously controlled to minimize the adverse effects.

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Section: Discussionmentioning

confidence: 99%

Evaluation of the effects of Bambusa tulda on osteogenic differentiation and mineralization of human stem cells

Lee¹,

Uddin²,

Lee³

et al. 2018

biomedicalresearch

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show abstract

“…Stem cells may be obtained from various tissues, including bone marrow and adipose tissue (3,4). Stem cells derived from gingiva may be useful for research and treatment of diseases as harvesting stem cells from the mandible or maxilla may be performed easily under local anesthesia (5,6). Human mesenchymal stem cells have previously been isolated and characterized from the gingiva with minimal complications (7).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva-derived stem cells and osteoprecursor cells

Lee

Park

2017

Experimental and Therapeutic Medicine

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The present study was performed to create stem cell spheroids from human gingiva-derived stem cells and osteoprecursor cells and to evaluate the maintenance of the stemness, the viability and osteogenic differentiation of the cell spheroids. Gingiva-derived stem cells were isolated, and a total of 6×105 stem cells and osteoprecursor cells were seeded into concave micromolds at various ratios. Gingiva-derived stem cells and/or osteoprecursor cells formed spheroids in concave microwells. The spheroids demonstrated a smaller diameter when the number of osteoprecursor cells seeded was lower. The majority of cells in the spheroids were identified to be live cells and the cell spheroids preserved viability throughout the experimental period. The cell spheroids, which contained stem cells, were positive for stem-cell markers. Cell spheroids in concave microwells demonstrated a statistically significant increase in alkaline phosphatase activity as time progressed (P<0.05). A statistically significant difference in phosphatase activity was observed in the stem cell alone group when compared with the osteoprecursor cell group at day 5 (P<0.05). Mineralized extracellular deposits were observed in each group after Alizarin Red S staining. Within the limits of the present study, cell spheroids from gingival cells and osteoprecursor cells maintained shape, viability, stemness and osteogenic differentiation potential.

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“…3) Moreover, in recent years, the effects of medicinal herbs on stem cells have been tested. [3][4][5] The application of Cimicifugae rhizoma increased cell proliferation without morphologic changes. 5) Similarly, Angelicae dahuricae radix produced a marginal increase in stem cell proliferation.…”

Section: Introductionmentioning

confidence: 99%

“…5) Similarly, Angelicae dahuricae radix produced a marginal increase in stem cell proliferation. 4) The effect of differentiation of stem cells has been of interest. A previous report suggested that the therapeutic potential of stem cells has limitations due to cell survival and differentiation capabilities.…”

Section: Introductionmentioning

confidence: 99%

The effects of on osteogenic differentiation and mineralization of human stem cells derived from the gingiva

Lee

Uddin²,

Kim

et al. 2017

J Korean Med

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The aim of the present study is to evaluate the effects of the extract of Pongamia pinnata on the morphology, viability, and differentiation potential of human stem cells derived from the gingiva. Methods: Stem cells obtained from gingivae were cultured in an osteogenic medium in the presence of methanol extract of Pongamia pinnata (PPT) at concentrations ranging from 0.001 to 1%. Evaluations of cell morphology and cellular viability were done at Day 1. Alkaline phosphatase activity assays and Alizarin red S staining were performed to evaluate the osteogenic differentiation of stem cells. Results: The morphology of stem cells in the presence of PPT at final concentrations of 0%, 0.001%, 0.01%, 0.1%, and 1% did not produce any noticeable changes when compared with the untreated control group. Application of PPT produced a significant increase in alkaline phosphatase activity when compared to the control group. The results of the Alizarin Red S staining showed a significant increase of absorbance with the 0.001% group. Conclusions: Based on these findings, it was concluded that PPT could produce beneficial effects on mesenchymal stem cells with enhanced osteogenic differentiation.

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Evaluation of the effects of Angelicae dahuricae radix on the morphology and viability of mesenchymal stem cells

Cited by 20 publications

References 19 publications

Evaluation of the effects of Bambusa tulda on osteogenic differentiation and mineralization of human stem cells

Evaluation of the effects of Bambusa tulda on osteogenic differentiation and mineralization of human stem cells

Evaluation of the shape, viability, stemness and osteogenic differentiation of cell spheroids formed from human gingiva-derived stem cells and osteoprecursor cells

The effects of on osteogenic differentiation and mineralization of human stem cells derived from the gingiva

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