The effect of sodium selenite and feed additive “Metisevit plus” on the protein-synthesizing function and functional state of the liver of rats under prolonged cadmium and lead loading

Slobodian, S. О.; Gutyj, B. V.; Gufriy, D. F.; Hnativ, P. S.; Murska, S. D.

doi:10.32718/nvlvet10010

Cited by 4 publications

(4 citation statements)

References 8 publications

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“…High content of lead and cadmium was determined in fodders of Nyva Cooperative of Dubno district and "Ukraina" Enterprise of Dubrovytsia district. At the same time, a great difference was determined in accumulations of these metals between different types of fodder (Slivinska et al, 2019;Slobodian et al, 2020).…”

Section: Discussionmentioning

confidence: 99%

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Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Slobodian

Gutyj

Darmohray

et al. 2021

Regul. Mech. Biosyst.

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Prolonged ingress of heavy metals into the organisms of animals, even in low concentrations, causes a number of toxic impacts, affecting various organs and systems. The objective of this study was to research parameters of enzymatic and non-enzymatic links of the system of antioxidant protection of the organism of young cattle in the condition of lead-cadmium load and the effect of Metisevit Plus feed additive. Studies were conducted on the basis of the Private Agricultural Company “Ukraina”, located in Dubrovytsia district of Rivne Oblast, using 12 six-month old bulls of Ukrainian Black Pied Dairy breed divided into two groups, each consisting of 6 animals. Bulls of the control group received the standard diet. The bulls of the experimental group were fed with Metisevit Plus feed additive in the dose of 0.5 g/kg of feed. The contents of lead and cadmium in feed at the enterprise were determined to be high. According to the conducted studies, it was determined that in conditions of cadmium and lead load, there is a decrease in activity of glutathione link of the system of antioxidant protection of the organism of bulls. Feeding the bulls of the experimental group with Metisevit Plus feed additive for 30 days led to increase in the levels of non-enzymatic and enzymatic activities of the glutathione system. Significant increase in the level of reduced glutathione was recorded on days 30 and 40 of the experiment. Consumption of Metisevit Plus by the animals of the experimental group promoted increase in the activity of catalase and superoxide dismutase starting on the 20th day of the experiment. On days 30 and 40 of the experiment, the activity of the studied enzymes ranged within the physiological values. Therefore, the studies conducted on the bulls indicate that in the conditions of cadmium and lead load the feed additive Metisevit Plus fed to the bulls activated the system of antioxidant protection by increasing the levels of enzymatic and non-enzymatic links. When feeding bulls of the experimental group with Metisevit Plus feed additive, we determined significant decreases in diene conjugates and TBA-active products in their blood starting already on the 10th day of the experiment. On days 20 and 30 of the experiment, the level of diene conjugates in the blood of animals of the experimental group decreased by 14.5% and 24.0%. Efficiency of the feed additive may be explained by the effect of its constituents on the main pathogenic links of the pathological process caused by cadmium and lead. As indicated in the studies, liquidation of the syndrome of endogenous intoxication restores the functional condition of cellular membranes of the liver, protein-synthesizing function of the liver, increases the antioxidant status of the organisms of bulls. The studies we conducted confirm the expedience of using feed additive Metisevit Plus to prevent lead-cadmium toxicosis.

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Section: Discussionmentioning

confidence: 99%

“…The effectiveness of the feed additive may be explained by the impact of its constituents based on the pathogenic link of the pathological process caused by cadmium and lead (Slobodian et al, 2020). Inclusion of selenium in the composition of feed additive prevented the absorption of cadmium and lead in the digestive tract.…”

Section: Discussionmentioning

confidence: 99%

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Slobodian

Gutyj

Darmohray

et al. 2021

Regul. Mech. Biosyst.

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“…Ефективність кормової добавки можна пояснити впливом її складників на основні патогенетичні ланки патологічного процесу, спричиненим Кадмієм і Свинцем (Slobodian et al, 2020).…”

Section: рисunclassified

Influence of “Metisevit Plus” feed additive on morphological and biochemical parameters of bull blood under conditions of lead-cadmium loading

Slobodian¹,

Gutyj²,

Shalovylo³

et al. 2022

SMLNUVMB

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In the conditions of modern man-caused pollution of the environment, environmental problems, as well as improving the quality of livestock products and their food safety, are important and relevant issues today. The study aimed to investigate the effect of the “Metisevit Plus” feed additive on bull blood's morphological and biochemical parameters under lead-cadmium loading conditions. The research was conducted based on the agricultural private enterprise “Ukraine” of Dubrovytsia district of Rivne region on 12 bulls of six months of age, Ukrainian black-and-white dairy breed, which was formed into two groups of 6 animals each. The bulls of the control group were on a standard diet. The bulls of the experimental group were fed the feed additive “Metisevit Plus” at a dose of 0.5 g/kg of feed. This farm has a high content of lead and cadmium in feed. According to the results of experimental studies, it was found that the feed additive “Metisevit Plus” is effective under lead-cadmium load in bulls. Administration of this feed additive to experimental animals helps restore their suppressed hematopoietic function; the number of erythrocytes and hemoglobin in their blood increased by 25.3 and 19.4 %, and the number of leukocytes decreased by 12.4 %, respectively. Metisevit Plus feed additive also enhanced the functional state and protein-synthesizing function of the liver of bulls under artificial conditions. When feeding the feed additive “Metisevit Plus”, a decrease in the activity of both alanine aminotransferase and aspartate aminotransferase in the serum of bulls of the experimental group was found. On day 40 of the experiment, it was found that the activity of alanine and aspartate aminotransferase in the serum of bulls of the experimental group fluctuated within physiological values. When a “Metisevit-Plus” feed additive is added to the diet, there is a tendency to increase the total protein level in bulls of the experimental group. In the study of the albumin level in the blood of bulls of the experimental group, its probable increase was found starting from the 10th day of the experiment. On the 30th and 40th day of the experiment, the albumin level in the blood of bulls in the experimental group was the highest, whereas compared to the control group, it increased by 15.4 and 17.0 %, respectively. Our studies confirm the feasibility of using the feed additive “Metisevit Plus” to prevent lead-cadmium toxicosis.

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“…Високий вміст Свинцю і Кадмію встановлено в кормах СВК "Нива" Дубенського району та СП "Україна" Дубровицького району. Одночасно виявлено значну відмінність у нагромадженні цих металів між різними видами кормів (Slivinska et al, 2019;Slobodian et al, 2020).…”

Section: вступunclassified

Influence of Metisevit Plus feed additive on the activity of the glutathione system of the body of bulls under conditions of man-caused load

et al. 2021

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Pollution of the environment with heavy metals due to artificial activities of the population has led to several problems for agricultural production. The work aims to study the influence of Metisevit Plus feed additive on the state of the glutathione system of the body of bulls under conditions of man-caused load. The research was conducted based on the private agricultural enterprise “Ukraine” Dubrovytsia district of Rivne region on 12 bulls six months old, Ukrainian black-and-white dairy breed, which was formed into two groups of 6 animals each: 1 group – control, bulls were on a standard diet; Group 2 – experimental, bulls were fed a feed additive “Metisevit plus” at a dose of 0.5 g/kg of feed. According to studies, cadmium and lead load reduce the activity of the glutathione link in the antioxidant defense system of bulls. Feeding the Metisevit Plus feed additive to the bulls of the experimental group for 30 days helped increase the level of non-enzymatic and enzymatic activity of the glutathione system. A probable increase in the level of reduced glutathione was found on 30 and 40 days of the experiment, were compared with the control group of animals, it increased by 7.2 and 7.1 %, respectively. In the study of the activity of glutathione peroxidase and glutathione reductase, it was found that in these study periods they ranged from 35.7 ± 1.61 and 35.3 ± 1.55 nmol NADPH/min per 1 mg of protein (P ≤ 0.01) and 1.67 ± 0.041 and 1.64 ± 0.039 nmol NADPH/min per 1 mg of protein (P ≤ 0.001). Thus, studies on bulls indicate that the feed additive Metisevit Plus, when fed to bulls, under a load of cadmium and lead, contributed to the activation of the glutathione antioxidant defense system by increasing the level of enzymatic and non-enzymatic units. Our studies confirm the feasibility of using a feed additive, Metisevit Plus, to prevent lead-cadmium toxicosis.

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The effect of sodium selenite and feed additive “Metisevit plus” on the protein-synthesizing function and functional state of the liver of rats under prolonged cadmium and lead loading

Cited by 4 publications

References 8 publications

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Antioxidant status of the organisms of young bulls in the conditions of lead-cadmium load and effect of correcting factors

Influence of “Metisevit Plus” feed additive on morphological and biochemical parameters of bull blood under conditions of lead-cadmium loading

Influence of Metisevit Plus feed additive on the activity of the glutathione system of the body of bulls under conditions of man-caused load

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