The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells

Chang, Yu‐Chao; Huang, Fu‐Mei; Tai, Kuo‐Wei; Chou, Ming‐Yung

doi:10.1067/moe.2001.116812

Cited by 188 publications

(168 citation statements)

References 24 publications

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“…8), accordingly to what has been reported in previous studies (Chang et al 2001;Hidalgo et al 2001). This result was confirmed by cell morphology and integrity inspection based on differential AO/EB staining.…”

Section: Antimicrobial Activity Of Chx-compounded Pcl Fibressupporting

confidence: 90%

“…On the other hand, neither antibiotic nor toxic effect is reported for PCL. The results of trypan blue exclusion assay and cell morphology and integrity inspection based on differential AO/EB staining showed that the exposure to fibre incorporating the higher amount of CHX does not impair cell viability, while exposure to pure CHX or 100°C-treated CHX reduces cell viability to 1 %, accordingly to what has been reported in previous studies (Chang et al 2001;Hidalgo et al 2001). This finding might be due to the CHX release kinetics from compounded PCL fibres.…”

Section: Discussionsupporting

confidence: 80%

“…At CHX concentrations of 10-20 μg/ml or more, a bactericidal effect can be expected as well as inhibitory growth property against yeasts (Russell and Day 1993). Studies evaluating in vitro toxicity on human cell revealed that CHX concentrations greater than 0.0001 % significantly compromise cell viability after a 24-h exposure (Chang et al 2001;Hidalgo et al 2001). On the other hand, neither antibiotic nor toxic effect is reported for PCL.…”

Section: Discussionmentioning

confidence: 99%

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Combining in the melt physical and biological properties of poly(caprolactone) and chlorhexidine to obtain antimicrobial surgical monofilaments

Scaffaro

Botta

Sanfilippo

et al. 2012

Appl Microbiol Biotechnol

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Bacterial infections on a sutured wound represent a critical problem, and the preparation of suture threads possessing antimicrobial properties is valuable. In this work, poly(caprolactone) (PCL) monofilaments were compounded at the concentration of 1, 2 and 4 % (w/w), respectively, to the antiseptic chlorhexidine diacetate (CHX). The incorporation was carried out in the melt by a single-step methodology, i.e. "online" approach. Mechanical tests revealed that the incorporation of CHX does not significantly change tensile properties of PCL fibres as the thermal profile adopted to prepare the compounded fibres does not compromise the antibacterial activity of CHX. In fact, CHX confers to compounded PCL fibres' antimicrobial property even at the lowest CHX concentration as revealed by microbiological assays performed on Escherichia coli, Micrococcus luteus and Bacillus subtilis strains. The scanning electron microscope micrographs and energy-dispersive X-ray analysis of compounded threads revealed that CHX is uniformly distributed on fibre surface and that the overall amount of superficial CHX increases by increasing compounded CHX concentration. This distribution determines a biphasic CHX release kinetics characterized by an initial rapid solubilisation of superficial CHX micro-crystals, followed by a slow and gradual release of CHX incorporated in the bulk. Interestingly, the compounded threads did not show any toxic effect compromising cell viability of human fibroblasts in vitro, differently from that observed using an equal amount of pure CHX. Thus, this study originally demonstrated the effectiveness of an "online" approach to confer antimicrobial properties to an organic thermoplastic polymeric material commonly used for medical devices.

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Section: Antimicrobial Activity Of Chx-compounded Pcl Fibressupporting

confidence: 90%

Section: Discussionsupporting

confidence: 80%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Combining in the melt physical and biological properties of poly(caprolactone) and chlorhexidine to obtain antimicrobial surgical monofilaments

Scaffaro

Botta

Sanfilippo

et al. 2012

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…However, Hidalgo et al (2) using an XTT assay, observed cell death in cultured skin fibroblasts after application of NaOCl at concentrations >0.05% for 2-24h. Furthermore, Chang et al (19), using a PI fluorescence assay, found that 0.4% and 0.2% dilutions of a 5.25% NaOCl solution killed human periodontal ligament cells after 3 and 24h of exposure, respectively. Discrepancies with the present results may be due to the use of different cell lines, procedures, and experimental conditions used to assess cytotoxicity.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy

Navarro-Escobar¹,

González-Rodríguez²,

Ferrer-Luque³

2009

Med Oral

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Navarro-Escobar E, González-Rodríguez MP, Ferrer-Luque CM. Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy. Med Oral Patol Oral Cir Bucal. 2010 Jan 1;15 (1):e90-4.http://www.medicinaoral.com/medoralfree01/v15i1/medoralv15i1p90.pdf AbstractAim: To evaluate the cytotoxicity of 15% citric acid, 5% phosphoric acid and 2.5% NaOCl on cultured fibroblasts using MTT colorimetric assay. Methodology: Irrigating solutions of 5% phosphoric acid, 15% citric acid, and 2.5% NaOCl, diluted at 0.1% and 0.5%, were applied to cell cultures of 3T3L1 fibroblasts. The cell viability was determined by means of MTT colorimetric assay after a period of 1, 6 and 24 hours. Percentages of cell viability were analyzed using the Kruskal-Wallis test for global comparisons and the Mann-Whitney U-test for pairwise comparisons. Results: The percentage of cell viability diminished progressively over a 24 hour period in all solutions at both dilutions. At 0.1% dilution, 2.5% NaOCl (63.39%) and 15% citric acid (53.91%) showed the highest percentage of cell viability (p=0.083). At 0.5% dilution, 2.5% NaOCl again showed the highest cell viability value (48.51%). Conclusions: The irrigating solution with the highest percentage of cell viability was 2.5% NaOCl at both 0.1% and 0.5% dilutions. A very low percentage of cell viability was obtained with 15% citric acid and 5% phosphoric acid at 0.5% dilution.

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“…The earliest mention of medicinal use of plants in Hindu culture is found in "Rigveda". Herbs like neem and green tea might have a potential use as irrigants as they have been found to possess antimicrobial and antifungal properties 5,7 . Neem has been extensively used in ayurveda, unani and homoeopathic medicine.…”

Section: Introductionmentioning

confidence: 99%

Antifungal efficacy of Azadirachta indica (neem) - An in vitro study

Raghavendra¹,

Balsaraf²

2014

Braz. J. Oral Sci.

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Aim: To evaluate antimicrobial ability of neem leaf extract, 3% sodium hypochlorite (NaOCl) and 2% chlorhexidine (CHX) against Candida albicans. Methods: Neem leaf extract was prepared by using absolute ethanol with fresh neem leaves, filtering the extract through muslin cloth, coarse residue and filter paper. Cultures of C. albicans were maintained on Brain Heart Infusion broth and Agar. The antimicrobial efficacy was checked using the Agar Diffusion and the zones of inhibition were measured. The results were statistically analysed using ANOVA test. Inter-group comparison was checked using Kruskal Wallis ANOVA and Mann Whitney tests (a=0.05). Results: There was statistically significant difference between the zones of inhibition seen with 3% NaOCl and Neem extract with 2% CHX (p<0.05). There was no significant difference observed between Neem extract and 3% NaOCl. Conclusions: Efficacy of Neem extract is comparable to 3% NaOCl against C. albicans and it is significantly better than 2% CHX.

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The effect of sodium hypochlorite and chlorhexidine on cultured human periodontal ligament cells

Cited by 188 publications

References 24 publications

Combining in the melt physical and biological properties of poly(caprolactone) and chlorhexidine to obtain antimicrobial surgical monofilaments

Combining in the melt physical and biological properties of poly(caprolactone) and chlorhexidine to obtain antimicrobial surgical monofilaments

Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy

Antifungal efficacy of Azadirachta indica (neem) - An in vitro study

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