Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy

Abstract

Navarro-Escobar E, González-Rodríguez MP, Ferrer-Luque CM. Cytotoxic effects of two acid solutions and 2.5% sodium hypochlorite used in endodontic therapy. Med Oral Patol Oral Cir Bucal. 2010 Jan 1;15 (1):e90-4.http://www.medicinaoral.com/medoralfree01/v15i1/medoralv15i1p90.pdf AbstractAim: To evaluate the cytotoxicity of 15% citric acid, 5% phosphoric acid and 2.5% NaOCl on cultured fibroblasts using MTT colorimetric assay. Methodology: Irrigating solutions of 5% phosphoric acid, 15% citric acid, and 2.5% NaOCl, diluted at 0.1% and 0.5%, were applied to cell cultures of 3T3L1 fibroblasts. The cell viability was determined by means of MTT colorimetric assay after a period of 1, 6 and 24 hours. Percentages of cell viability were analyzed using the Kruskal-Wallis test for global comparisons and the Mann-Whitney U-test for pairwise comparisons. Results: The percentage of cell viability diminished progressively over a 24 hour period in all solutions at both dilutions. At 0.1% dilution, 2.5% NaOCl (63.39%) and 15% citric acid (53.91%) showed the highest percentage of cell viability (p=0.083). At 0.5% dilution, 2.5% NaOCl again showed the highest cell viability value (48.51%). Conclusions: The irrigating solution with the highest percentage of cell viability was 2.5% NaOCl at both 0.1% and 0.5% dilutions. A very low percentage of cell viability was obtained with 15% citric acid and 5% phosphoric acid at 0.5% dilution.

Toxicity of irrigating solutions and pharmacological associations used in pulpectomy of primary teeth

“…These results are in agreement with Navarro‐Escobar et al . (), who evaluated cell viability by MTT at 24 h of 2.5% NaOCl in two dilutions, 0.1% and 0.5% on fibroblasts (NIH/3T3) and also found a significant reduction in 0.5%, only 0.1% dilution had higher levels of cell viability. Regarding CHX, Lessa et al .…”

Complications following an accidental sodium hypochlorite extrusion: A report of two cases

“…Thus, several studies have been carried out in order to compare the cytotoxic effects with those of other irrigant solutions. Regarding this aspect, Navarro-Escobar et al (12) compared the cytotoxicity of 15% citric acid, 5% phosphoric acid and 2.5% NaOCl. They concluded that the irrigating solution with the highest percentage of cell viability was 2.5% NaOCl at both 0.1% and 0.5% dilu-tions.…”

Antimicrobial Effectiveness of Intracanal Ozone Treatment

“…Ishod endodontskog zahvata tako ovisi o vrsti i broju zaostalih mikroorganizama (5). Najčešće rabljeno sredstvo za dezinfekciju i irigaciju korijenskih kanala jest natrijev hipoklorit (NaOCl).…”

“…The outcome of treatment depends on the type and number of residual microorganisms (5). The most commonly used root canal irrigation solution is sodium hypochlorite (NaOCl).…”