Toxicity of irrigating solutions and pharmacological associations used in pulpectomy of primary teeth

Botton, Graziela; Pires, Carine Weber; Cadoná, Francine Carla; Machado, A. K.; Azzolin, Verônica Farina; Cruz, Ivana Beatrice Mânica da; Sagrillo, Michele Rorato; Praetzel, Juliana Rodrigues

doi:10.1111/iej.12509

Cited by 28 publications

(29 citation statements)

References 39 publications

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“…Furthermore due to the low level of hazardousness 11 , 17 and the high level of biocompatibility 8 , it might directly affect the healing process. Studies have proven its ability to interact effectively with microbiota in the root canal system and therefore to eliminate microorganisms 1 , 3 , 5 - 7 , 10 , 12 - 17 . This feature is so remarkable that bacterial resistance has not been reported in the scientific literature.…”

Section: Introductionmentioning

confidence: 99%

Ozone therapy as an adjuvant for endondontic protocols: microbiological – ex vivo study and citotoxicity analyses

Nogales¹,

Ferreira²,

MONTEMOR³

et al. 2016

J. Appl. Oral Sci.

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Objectives This study evaluated the antimicrobial efficacy of ozone therapy in teeth contaminated with Pseudomonas aeruginosa, Enterococcus faecalis, and Staphylococcus aureus using a mono-species biofilm model. Parallel to this, the study aimed to evaluate the cytotoxicity of ozone for human gingival fibroblasts. Material and Methods: One hundred and eighty single-root teeth were contaminated with a mono-species biofilm of Enterococcus faecalis, Pseudomonas aeruginosa, and Staphylococcus aureus. Groups were formed: Group I – control; Group II – standard protocol; Group III – standard protocol + ozone gas at 40 µg/mL; and Group IV – standard protocol + aqueous ozone at 8 µg/mL. In parallel, human gingival fibroblasts were submitted to the MTT test. Cells were plated, then ozone was applied as follows: Group I (control) – broth medium; Group II – aqueous ozone at 2 µg/mL; Group III – aqueous ozone at 5 µg/mL; and Group IV – aqueous ozone at 8 µg/mL. Data were submitted to the Kruskal Wallis test and Bonferroni post hoc analyses to assess microbiology and cytotoxicity, respectively (p<0.05%).Results The results revealed antimicrobial efficacy by Group IV with no CFU count. The cytotoxicity assay showed Groups III and IV to be the most aggressive, providing a decrease in cell viability at hour 0 from 100% to 77.3% and 68.6%, respectively. Such a decrease in cell viability was reverted, and after 72 hours Groups III and IV provided the greatest increase in cell viability, being statistically different from Groups I and II.Conclusion According to the applied methodology and the limitations of this study, it was possible to conclude that ozone therapy improved the decontamination of the root canal ex vivo. Ozone was toxic to the cells on first contact, but cell viability was recovered. Thus, these findings suggest that ozone might be useful to improve root canal results.

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Section: Introductionmentioning

confidence: 99%

Ozone therapy as an adjuvant for endondontic protocols: microbiological – ex vivo study and citotoxicity analyses

Nogales¹,

Ferreira²,

MONTEMOR³

et al. 2016

J. Appl. Oral Sci.

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“…Human primary second mandibular molar teeth recently extracted were collected from patients aged [5][6][7][8] , due to periapical pathology and orthodontic reasons, and stored in distilled water at 4 o C. Multidimensional preoper-ative radiographs were taken to determine root curvature less than 30 o using the Schneider method 26 , and the presence of single, noncomplicated canal of distal roots. The determination of the amount of root resorption was performed using the formula described by Rajan.…”

Section: Specimen Preparationmentioning

confidence: 99%

“…[4][5][6] Because of the large number of accessory canals located in the furcation area of deciduous teeth, 7 irrigation solutions can easily pass into the furcation area, which poses a high risk for permanent tooth germ exists in that area. 8 Klein and Kleier 9 reported in their case report of a 10-year old patient that a sodium hypochlorite accident is a rare event in adults, but even more so in children. Also, Siqueira 10 reported the prevalence of complications associated with NaOCl ranging from 1% to 16% in adult patients.…”

Section: Introductionmentioning

confidence: 99%

“…13 Thus, prevention of the cytotoxic effects of apically extruded debris and irrigants on the periapical tissues and tooth germs is a relevant factor for safety endodontic procedures in pediatric root canal therapy. 8 Although conventional needle irrigation (CNI) is the most widely used irrigation technique in endodontic procedures, several irrigation techniques and devices are being used to improve the disinfection of root canal system.…”

Section: Introductionmentioning

confidence: 99%

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The effect of four irrigation techniques on the amount of apically extruded debris in pulpectomy of primary teeth

Buldur¹,

Kapdan²

2018

Yeditepe J Dent

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SUMMARY Aim:The aim of this in vitro study was to compare the amount of apically extruded debris of primary molar root canals during pulpectomy using four irrigation techniques. Materials and Methods: Root canals of eighty primary second molar teeth were instrumented using ProTaper Next rotary system and randomly divided into 4 experimental groups (n=20): group I -EndoVac, group II-manual dynamic agitation (MDA), group III-Self Adjusting File (SAF), and group IV-conventional needle irrigation (CNI). Bidistilled water was used as irrigant and the apically extruded debris was collected and dried in preweighed Eppendof tubes. Data were analyzed statistically using the ANOVA and the Bonferroni post hoc t-test (p=0.05). Results: EndoVac and SAF extruded significantly less debris than did MDA and CNI (p<0.05), while no statistically significant difference was found between EndoVac and SAF (p>0.05). Conclusions: Within the limitations, all irrigation systems extruded debris. EndoVac and SAF extruded less debris compared with MDA and CNI.

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“…Sodium hypochlorite, chlorhexidine, gluconate, citric acid and EDTA, which are all among irrigation solutions used in deciduous teeth, were compared with respect to cytotoxicity, and chlorhexidine gluconate was reported to have a lower level of toxic effect than others (34).…”

Section: Canal Irrigation Agentsmentioning

confidence: 99%

Root Canal Treatment for Deciduous Teeth: A Review

Daloğlu¹,

Güzel²

2017

meandros

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Süt dişlerinin erken kaybı fizyolojik, fonksiyonel ve estetik açıdan birçok olumsuzluğa yol açmaktadır. Süt dişlerinin yapısı, çürüğün çok daha kolay ve kısa sürede ilerlemesine sebep olmakta ve bu dişlerin ağızda tutulmaları için sıklıkla pulpa tedavilerine ihtiyaç duyulmaktadır. Süt dişi kök kanal tedavilerinin başarısını arttıracak yeni materyallerin araştırılmasına devam edilmektedir. Çalışmamızda süt dişi kök kanal tedavilerinde son dönemde öne çıkan güncel yaklaşımların derlenmesi amaçlanmıştır. Öz AbstractThe early loss of deciduous teeth leads to many negations in terms of physiological, functional and aesthetic. The structure of the deciduous teeth causes decay to progress much easier and less time and this is the need for frequent dental pulp treatment to be kept in the mouth. The new materials that will increase the success of deciduous teeth root canal treatment are continuing to investigate. In this study, it is aimed that recently prominent current approaches are to be compiled in root canal treatment of deciduous teeth.

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Toxicity of irrigating solutions and pharmacological associations used in pulpectomy of primary teeth

Abstract: All groups showed some level of toxicity. Amongst the main solutions, chlorhexidine presented less cytotoxic potential. EDTA was the least cytotoxic of the auxiliary irrigant solutions, and the association of these two solutions showed the lowest toxicity potential amongst all groups.

Cited by 28 publications

References 39 publications

Ozone therapy as an adjuvant for endondontic protocols: microbiological – ex vivo study and citotoxicity analyses

Ozone therapy as an adjuvant for endondontic protocols: microbiological – ex vivo study and citotoxicity analyses

The effect of four irrigation techniques on the amount of apically extruded debris in pulpectomy of primary teeth

Root Canal Treatment for Deciduous Teeth: A Review

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