The Effect of Slow-grown in Vitro Storage Under Different Light Spectra on Banana Plantlets Cv. Prata Catarina (AAB).

Rodrigues, Paulo Hercílio Viegas; Oliveira, Emerson; Demétrio, Christian Aparecido; Ambrosano, Guilherme Bovi; Piedade, Sônia M.

doi:10.21203/rs.3.rs-597268/v1

Cited by 3 publications

(3 citation statements)

References 21 publications

(29 reference statements)

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“…without compromising explant survival and with a lower risk of inducing genetic variations (Muñoz et al, 2019;Viegas et al, 2021). For this species, a storage period of 12 months without subcultures was evaluated.…”

Section: Responses To Slow-growth Storagementioning

confidence: 99%

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

Armijos-Gonzalez,

Ramón-Contento,

Cueva-Agila

2023

Preprint

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Cinchona officinalis has experienced anthropogenic pressures for nearly 400 years, such as overexploitation, habitat fragmentation, and the subsequent reduction of genetic diversity. Additionally, the challenge of regeneration in its natural environment makes it a vulnerable species. In this context, various treatments for the in vitro conservation of explants were evaluated in the present study. For short-term conservation, the effects of osmotic substances such as sorbitol, mannitol, and sucrose at different concentrations were assessed. Different concentrations of MS and B5 culture media were also examined for their impact on the growth, budding, mortality, and rooting of explants over 12 months without subcultures. For long-term conservation by cryopreservation, two techniques were tested: vitrification and encapsulation-dehydration. Short-term preservation of explants in sorbitol resulted in low mortality, minimal growth, and limited development of new shoots compared to preservation in sucrose or mannitol, although tissues could be recovered successfully from all storage conditions. After cryopreservation and 45 days of recovery, explants with the lowest mortality (4%) were from the control treatment, cultivated in a medium with sucrose which proved useful as a cryoprotectant, followed by the encapsulation-dehydration treatment. In conclusion, it is possible to conserve C. officinalis tissues in the short-term using in vitro techniques, while further assays are needed for long-term conservation.

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Section: Responses To Slow-growth Storagementioning

confidence: 99%

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

Armijos-Gonzalez,

Ramón-Contento,

Cueva-Agila

2023

Preprint

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“…Local and global efforts for the ex situ preservation of banana germplasm are massive. For example, at the International Transit Centre (ITC) in Leuven (Belgium), the in vitro SGS is achieved under a combination of low temperature (16 °C) and limited light (25 μmol m −2 s −1 ) [ 71 , 72 ]. Therefore, this method is appropriate to decrease the subculture frequency, ranging between 3 and 22 months, depending on the genomic group and in vitro browning reaction [ 71 ].…”

Section: Conservation In Sgs Of Tropical Fruit Speciesmentioning

confidence: 99%

“…An innovative study evaluated the effect of different spectra of light on the conservation of the banana ‘Prata Catarina’ group AAB under SGS [ 72 ]. Plantlets maintained with a photoperiod of 14 h (25 °C) were illuminated with red and blue light (combined or alone) or with white light.…”

Section: Conservation In Sgs Of Tropical Fruit Speciesmentioning

confidence: 99%

In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

Benelli̇

Tarraf

İzgü

et al. 2022

Plants

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Plant genetic resources conservation may be a potential option for the improvement of agricultural crops through modern biotechnologies, and in vitro conservation is a tool available to safeguard plant biodiversity. Ex situ conservation of plant genetic resources using the in vitro procedures is in progress in many countries. The slow growth storage (SGS) technique is a valid in vitro approach to preserve several vegetatively propagated species by controlling the growth and development of plantlets, economizing storage space and labor and reducing costs. Moreover, SGS prolongs the timing between subcultures, lowers the risk of losing germplasm through handling errors, such as contamination problems, and decreases the risk of genetic instability due to the reduction in the number of subcultures. SGS is applied by considering different factors: temperature, light or darkness conditions, medium composition, including mineral or sucrose concentrations, and the presence/absence of plant growth regulators, osmotic agents and growth inhibitors. SGS protocols for some fruit species have been well defined, others require additional research. The present review focuses on the effect of several factors that influence the SGS of in vitro shoots derived from temperate and tropical fruit species during the last ten years.

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Cinchona officinalis L. ex situ conservation by in vitro slow growth and cryopreservation techniques

Armijos-González,

Ramón,

Cueva-Agila

2024

Plant Cell Tiss Organ Cult

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The Effect of Slow-grown in Vitro Storage Under Different Light Spectra on Banana Plantlets Cv. Prata Catarina (AAB).

Cited by 3 publications

References 21 publications

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

Cinchona officinalis L. in vitro conservation by means of slow-growth storage and cryopreservation techniques

In Vitro Conservation through Slow Growth Storage Technique of Fruit Species: An Overview of the Last 10 Years

Cinchona officinalis L. ex situ conservation by in vitro slow growth and cryopreservation techniques

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