The Effect of Resveratrol on the Quality of Extended Boar Semen During Storage at 17ºC

Abstract

The natural polyphenol resveratrol may be beneficial to many aspects of cell function and animal health, although its actions in the male reproductive system vary depending on animal species. This work investigates resveratrol effects on the quality of preserved boar semen during liquid storage at 17ºC. We used three approaches: 1) evaluation of conventional parameters of seminal quality, 2) measurement of specific response to capacitating stimuli, and 3) evaluation of mitochondria membrane potential and ATP content. Resveratrol supplementation causes i) a loss in the response of liquid stored boar spermatozoa to capacitating stimuli, ii) a decrease in the sperm ATP content and iii) a reduction in the mitochondrial membrane potential. Moreover, higher concentrations of resveratrol increase plasma membrane phospholipid disorder and reduce the percentage of motile spermatozoa. These results suggest that semen doses supplemented with resveratrol could be considered sub-fertile compared with semen stored hypothermically in standard conditions.

High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen

“…The inhibitory effect of resveratrol (Martín-Hidalgo et al, 2013) and quercetin (Johinke et al, 2014) on the sperm Kinematic of different species has been reported, particularly when used at high concentrations. In this study, with the exception of the oscillation index (WOB) at time 0h, treating goat semen with different concentrations (0,15,25,50,75 and 100µM) of trans-resveratrol or quercetin did not alter the sperm kinematics.…”

“…The differences between studies released with the use of resveratrol and quercetin polyphenols have been attributed to the dose and differences between species (Moretti et al, 2012;Martín-Hidalgo et al, 2013). According to the species, enzymes that are directly or indirectly linked to sperm motility maintenance, such as Ca 2+ -ATPase (Nass-Arden and Breitbart, 1990), F0F1-ATPase (Zheng and Ramirez, 2000) and cyclooxygenase (COX) (Kowalska et al, 2011), are inhibited to varying degrees.…”

Is Resveratrol Effective in Protecting Stallion Cooled Semen?

“…In the boar, various doses of RSV (10, 33, 66, and 100 mM) supplemented during semen preservation at 17 C for 7 days did not improve sperm quality parameters [22].…”

Epigallocatechin‐3‐gallate (EGCG) and green tea polyphenols do not improve stallion semen parameters during cooling at 4°C

“…We already demonstrated that resveratrol is ineffective for improving stallion sperm storage (Giaretta et al., ) and that it could even become dangerous if supplemented at high concentrations. Other authors obtained similar results in porcine sperm (Martin‐Hidalgo et al., ), concluding that this antioxidant is not suitable for sperm preservation.…”

“…Other authors obtained similar results in porcine sperm (Martin-Hidalgo et al, 2013), concluding that this antioxidant is not suitable for sperm preservation.…”