High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen

Abstract

The aim of this study was to evaluate the effect of different concentrations of trans-resveratrol or quercetin on the ability of goat sperm to withstand being frozen. Six pools of semen obtained from six male goats were processed with different concentrations of resveratrol or quercetin (Experiment 1: 0, 15, 25, 50, 75 or 100µM resveratrol; Experiment 2: 0, 15, 25, 50, 75 or 100µM quercetin) and frozen. After thawing, the semen was evaluated for sperm kinematics, plasma membrane and acrosome integrity, morphology and oxidative stress following 0 and 1h of incubation. Immediately after thawing (0h), wobble (oscillation index) in the groups treated with 100µM of quercetin or resveratrol was lower (P<0.05) than in those treated with 0 and 25µM resveratrol and 0µM quercetin, respectively. After 1h of incubation, the total motility in treatments with 15, 50 and 75µM quercetin, as well as the plasma membrane integrity in all quercetin concentrations were lower (P<0.05) than at 0h. In opposition, the linearity of semen samples treated with 100µM quercetin and the straightness of those treated with 75 and 100µM quercetin were lower (P<0.05) at 0h than at 1h after thawing. Thus, it can be concluded that resveratrol and quercetin at high concentrations (100µM) transiently reduce the wobble of goat sperm submitted to frozen storage, and that quercetin (75 and 100µM) increases the linearity and straightness over time, which can be favorable for fertility.

Supplementation of quercetin for advanced DNA integrity in bull semen cryopreservation

“…When sperm motility characteristics, plasma membrane integrity and sperm abnormalities were considered, treatment did not improve any fields tested. The results of the present study indicate that neither resveratrol nor Q improved sperm morphology, plasma membrane, acrosomal integrity (Silva et al., ) and sperm motility characteristics (Mortimer, ). The addition of either methionine or Q did not ensure any effect on the sperm motility characteristics VAP and VCL in a study of chilled rabbit sperm (Johinke, de Graaf, & Bathgate, ).…”

“…These flavonoid's positive effects have been related to various characteristics such as oxidative inhibition (Ben Abdallah, Fetoui, Zribi, Fakhfakh, & Keskes, ), metal chelating capability (Ferrali et al., ) and protection of DNA integrity (Seifi‐Jamadi, Kohram, Shahneh, Ansari, & Macías‐García, ). Supplementation of semen extender with Q has been reported to have beneficial antioxidant properties on post‐thaw characteristics in sperm cells of bulls (Tvrda et al., ), horses (Seifi‐Jamadi et al., ), boars (Kim et al., ), rams (Silva, Cajueiro, Silva, Soares, & Guerra, ) and bucks (Silva, Arruda, Silva, Souza, & Guerra, ). In other respects, more research is needed to optimise the use of Q in ROS associated with oxidative stress.…”

Quercetin attenuates H2O2‐induced toxicity of rooster semen during liquid storage at 4°C

“…Moreover, Silva et al. (), believed that addition of quercetin to goat semen would make no changes on sperm kinematics. On the other hand, inhibitory effects of quercetin supplementation (especially at high doses) on sperm kinematics were reported in ram and rabbit (Breitbart, Rubinstein, & Nass‐Arden, ; Johinke et al., ).…”

“…Quercetin scavenges free radicals and provides antioxidant barrier through the chelation of divalent cations and inhibition of ROS formation (Afanas'ev, Dcrozhko, Brodskii, Kostyuk, & Potapovitch, ; da Silva, Piskula, Yamamoto, Moon, & Terao, ). Antioxidant properties of quercetin on semen characteristics have been previously evaluated in human (Moretti et al., ), goat (Silva, Arruda, Silva, Souza, & Guerra, ), rat (Farombi et al., ), stallion (Gibb, Butler, Morris, Maxwell, & Grupen, ), rabbit (Johinke, de Graaf, & Bathgate, ) and ram (Silva, Cajueiro, Silva, Soares, & Guerra, ). Quercetin exerted positive effects on productive performance of laying hens that is linked to its oestrogen‐like activities (Yang et al., ).…”

Avaliação in vitro do sêmen congelado de carneiros com diluidor suplementado com miricetina

“…Diversos estudos vêm sendo realizados para avaliar os efeitos antioxidantes de compostos fenólicos sobre os espermatozoides (Martinez-Soto et al, 2010;Moretti et al, 2012;Silva et al, 2016). No entanto, não há relatos na literatura acerca da ação da miricetina em diluidores de congelação de sêmen de qualquer espécie.…”