The effect of resveratrol on oxidative ovary-damage induced by methotrexate in rats (Resveratrol oxidative ovary-damage)

Ata, Nahit; Kulhan, Nur Gözde; Külhan, Mehmet; Turkler, Can; Kiremitli, Tunay; Kiremitli, Sevil; Çimen, Ferda Keskin; Süleyman, Halis; Toprak, Veysel

doi:10.4149/gpb_20190281

Cited by 8 publications

(7 citation statements)

References 18 publications

(19 reference statements)

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“…In our study, histopathological evaluation revealed that ovarian damage in the MTX + RES group was significantly milder than in the MTX group In addition, the decrease in the number of follicles seen in MTX groups increased in MTX+RES groups. Similar to our study in the literature, Ata et al (36) reported the effects of RES on MTX-induced ovarian damage. In this study, RES was shown to protect the ovary from damage caused by MTX.…”

Section: Discussionsupporting

confidence: 92%

Resveratrol Siçanlarda Oksi̇dati̇f Stresi̇ Ve Apoptozu Baskilayarak Metotreksat Kaynakli Yumurtalik Hasarini Hafi̇fleti̇r

AKA

Acar

Özgöçmen

et al. 2022

SDÜ Tıp Fakültesi Dergisi

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Abstract Objective: The study aimed to evaluate the protective effects of resveratrol (RES) against methotrexate (MTX)-induced ovarian damage in female rats and were divided into 7 groups: Material and Methods: Control; MTX (15mg/kg-1st day); MTX (1st and 3rd day); MTX (1st, 3rd and 5th day); MTX (1st day) + RES (20mg/kg); MTX (1st and 3rd day) + RES; MTX (1st, 3rd and 5th day) + RES. Since there was only one animal left in groups 4 and 6 and no animals in group 7, these groups were excluded from the evaluation. Ovarian damage was evaluated by performing hematoxylin-eosin (H-E) and TUNEL staining on ovarian tissues of rats. TAS and TOS activities in ovarian tissues were evaluated spectrophotometrically. In addition, DNA damage in blood was evaluated by comet assay. Results: As a result of biochemical analyzes, TOS levels increased in MTX groups, group 2 and 3, compared to the control group, while TOS level decreased significantly in group 5. On the other hand, TAS level increased significantly in group 5 compared to groups 2 and 3. H-E staining results showed that MTX-induced damage improved with RES (group 5) treatment. Maximum positive staining in TUNEL image; observed in equal amounts in groups 2 and 3, then in group 5. In addition, the comet score, which indicates DNA damage, increased significantly in groups 2 and 3, while it decreased significantly in group 5. Conclusion: In line with the results of this study, it was observed that resveratrol may provide protective effects against methotrexate-induced ovarian damage due to its antioxidant properties.

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Section: Discussionsupporting

confidence: 92%

Resveratrol Siçanlarda Oksi̇dati̇f Stresi̇ Ve Apoptozu Baskilayarak Metotreksat Kaynakli Yumurtalik Hasarini Hafi̇fleti̇r

AKA

Acar

Özgöçmen

et al. 2022

SDÜ Tıp Fakültesi Dergisi

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“…It has been stated in previous studies that biochemical findings that reflect oxidative stress coincide with histopathological findings 14 . As the literature suggests, MDA and tGSH are biochemical parameters that commonly used to identify oxidative damage in the ovarian tissue 15,16 . Histopathological findings showing increased MDA and reduced tGSH levels in ovarian tissues were reported as indicative of progressive tissue damage in a previous study 17 .…”

Section: Discussionmentioning

confidence: 99%

The effect of taxifolin on oxidative ovarian damage and reproductive dysfunctions induced by antipsychotic drugs in female rats

İnce

Özer

Kadıoğlu

et al. 2021

J of Obstet and Gynaecol

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Aim Typical antipsychotics (TAPs) are commonly used to treat schizophrenia and bipolar disorder. However, extrapyramidal disorders, hyperprolactinemia, and reproductive dysfunctions have been observed in women during the use of TAPs. For this reason, less toxic and prolactin‐sparing atypical antipsychotic (AAP) drugs such as clozapine (CLN) have been developed. The aim of this study is to investigate the effect of taxifolin on possible ovarian and reproductive toxicity associated with CLN and haloperidol (HPL) in female Wistar albino rats. Methods The rats were grouped as healthy control group (HCG), CLN, HPL, taxifolin + clozapine (TCL), and taxifolin + haloperidol (THL). Drugs were administered to the groups for 28 days. At the end of that time, ovarian tissues of six rats from each group were taken for histopathological and biochemical analyses. Remaining six rats in groups were examined for evaluation of reproductive dysfunctions. Results Severe degeneration and vacuolization were observed in the primary, secondary, and primordial follicles of the ovarian tissues of CLN‐ and HPL‐treated groups, of which malondialdehyde (MDA) level was high and total glutathione (tGSH) level was low. In the taxifolin‐treated groups, taxifolin significantly prevented the increase of MDA level and decrease of tGSH level, and the severity of histopathological damage was found to be lower. In addition, it was found that taxifolin significantly prevented infertility and delay in pregnancy associated with CLN and HPL. Conclusions The results of this experiment suggest that taxifolin can be beneficial in treating oxidative ovarian damage, infertility, and reproductive dysfunctions induced by CLN and HPL.

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“…It has been reported that RES may stimulate ovarian activity and slow ovarian aging. In POF animal models, RES activates primordial follicles, increases the quantity of primary, primordial, and developing follicles, enhances granulosa cell proliferation, reduces ovarian inflammatory response, preserves the ovarian structure, and prevents oocyte and granulosa cell damage [55,56].…”

Section: Discussionmentioning

confidence: 99%

Ameliorative Effect of Citrus Lemon Peel Extract and Resveratrol on Premature Ovarian Failure Rat Model: Role of iNOS/Caspase-3 Pathway

et al. 2022

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Premature ovarian failure (POF) is described as a loss of oocytes and the absence of folliculogenesis and is considered an adverse effect of chemotherapeutic drugs, which leads to infertility. Subsequently, the existing inquiry was achieved by exploring the potential suspicious influences of lemon peel extract (LPE), and resveratrol (RES) on cyclophosphamide (CPA) induced-POF. The results showed that CPA-induced POF significantly decreased serum estradiol (E2) and progesterone levels, along with a considerable rise in serum luteinizing hormone (LH) and follicle-stimulating hormone (FSH) levels. Moreover, CPA administration to rats significantly increased the serum level of Malondialdehyde (MDA) and significantly lowered the levels of reduced glutathione (GSH) and superoxide dismutase (SOD); in addition, it increased nuclear factor kappa B (NF-κB) levels, tumor necrosis factor-α (TNF-α), as well as cyclooxygenase 2 (COX-2) with the spread expression of inducible nitric oxide synthase (iNOS) mRNA levels and caspase-3 (Casp3) levels in ovarian tissues versus the control rats. However, treatment with LPE and RES suppressed the triggering of NF- κB pathways, evidenced by a considerable reduction in Casp3 & iNOS mRNA expression level and significant ameliorative effects in all evaluated parameters, as confirmed by the histological and immunohistochemical investigation when comparing the model group. In overall findings, both lemon peel extract and resveratrol can mitigate the adverse effects of CPA-induced POF. Most crucially, its combination therapy is a promising pharmacological agent for this disease.

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The effect of resveratrol on oxidative ovary-damage induced by methotrexate in rats (Resveratrol oxidative ovary-damage)

Cited by 8 publications

References 18 publications

Resveratrol Siçanlarda Oksi̇dati̇f Stresi̇ Ve Apoptozu Baskilayarak Metotreksat Kaynakli Yumurtalik Hasarini Hafi̇fleti̇r

Resveratrol Siçanlarda Oksi̇dati̇f Stresi̇ Ve Apoptozu Baskilayarak Metotreksat Kaynakli Yumurtalik Hasarini Hafi̇fleti̇r

The effect of taxifolin on oxidative ovarian damage and reproductive dysfunctions induced by antipsychotic drugs in female rats

Ameliorative Effect of Citrus Lemon Peel Extract and Resveratrol on Premature Ovarian Failure Rat Model: Role of iNOS/Caspase-3 Pathway

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