The effect of probiotic microorganisms and bioactive compounds on chemically induced carcinogenesis in rats

Abstract

Diet interventions and natural bioactive supplements have now been extensively studied to reduce risks of colon cancer, which is one of the major public health problem throughout the world. The objective of our investigation was to study the effects of probiotic, prebiotic, nutritional plant extract, and plant oil on selected biochemical and immunological parameters in rats with colon cancer induced by N,N dimethylhydrazine (DMH). Male and female Wistar albino rats were were fed by a high-fat (HF) diet (10% fat in the diet) and were divided into 9 groups: Control group; PRO group -HF diet supplemented with probiotic Lactobacillus plantarum to provide 3 x 10 9 c.f.u. of strain/1 ml of medium; PRE group -HF diet supplemented with inulin enriched with oligofructose (2% of HF diet); HES group -HF diet supplemented with plant extract of Aesculus hippocastanum L. (1% of HF diet); OIL group -HF diet comprised Lini oleum virginale (2% of HF diet); and combination of probiotic microorganisms and bioactive compounds in the groups -PRO-PRE, PRO-HES, PRO-OIL, PRE-OIL. Carcinogenesis was iniciated with subcutaneous injection of DMH (20 mg/kg) two times at week interval and dietary treatments were continued for the six weeks. Application of probiotic microorganisms and bioactive compounds in all treated groups significantly decreased the activities of bacterial enzymes (p<0.001), the fecal bile acids concentration (p<0.01; p<0.001) and significantly increased serum TNFα level (p<0.001) in comparison to the control rats. The number of coliforms was reduced in PRO, PRO-PRE, PRO-OIL and PRE-OIL groups and significantly higher count of lactobacilli (p<0.05) was observed in PRO-PRE, PRO-OIL and PRE-OIL groups in compare with the controls. In conclusion, the results of this study indicate that probiotic microorganisms and bioactive compounds could exert a preventive effect on colon carcinogenesis induced by DMH.

Mechanisms and therapeutic effectiveness of lactobacilli

“…18 Lactobacilli are capable of competitively inhibiting carcinogen and mutagen formation, altering overall metabolism, adsorbing and removing toxic and mutagenic metabolites and producing protective metabolites. 19 In the context of colorectal cancer, the prevention mechanism exerted by probiotics may be a combination of different actions such as intestinal microbiota modification, [20][21][22][23][24][25][26] inactivation of cancerogenic…”

The role of probiotics and natural bioactive compounds in modulation of the common molecular pathways in pathogenesis of atherosclerosis and cancer

“…The results of a previous study indicate that probiotic microorganisms could exert a preventive effect on chemically induced colon carcinogenesis in rats. The application of Lactobacillus plantarum in combination with bioactive compounds was more effective in comparison to the administration of probiotic alone (Bertková et al 2010).…”

The effect of new probiotic strain Lactobacillus plantarum on counts of coliforms, lactobacilli and bacterial enzyme activities in rats exposed to N,N-dimethylhydrazine (chemical carcinogen)

“…Based on an experimental study, Bertková et al (2010) suggested that Hippocastani extractum siccum may be a useful candidate agent for colon cancer chemoprevention and treatment. They investigated the effect of probiotic microorganisms and selected bioactive compounds in different combinations on decreasing the concentration of Table 2.…”