The effect of new probiotic strain Lactobacillus plantarum on counts of coliforms, lactobacilli and bacterial enzyme activities in rats exposed to N,N-dimethylhydrazine (chemical carcinogen)

Abstract

The aim of the present study was to evaluate the effect of the new probiotic strain Lactobacillus plantarum on chemically induced carcinogenesis in rats. Sprague dowley rats (n = 33) were divided into control and experimental groups and were fed a conventional laboratory diet. In the experimental group, rats were treated with the probiotic at the dose of 1 × 10 9 CFU (colonyforming units)/ml. Two weeks after the beginning of the trial, N,N-dimethylhydrazine (chemical carcinogen) injections were applied s.c. at the dose of 21 mg/kg b.w., 5 × weekly. At the end of the 8-month experimental period, faeces samples were taken from the rats and used for laboratory analysis. The counts of lactobacilli and coliforms and bacterial enzyme activity were determined. The probiotic strain L. plantarum as single species or in combination with oil (Lini oleum virginale) decreased the count of total coliforms and increased lactobacilli in faeces of rats. Application of probiotic microorganisms significantly (P < 0.05) decreased the activities of bacterial enzymes (β-galactosidase and β-glucuronidase) compared to the control group rats. The results of this study indicate that probiotic microorganisms could exert a preventive effect on colon carcinogenesis induced by N,N-dimethylhydrazine.

The Effect of Using New Synbiotics on the Turkey Performance, the Intestinal Microbiota and the Fecal Enzymes Activity in Turkeys Fed Ochratoxin A Contaminated Feed

“…The research of Čokášová et al studied the probiotic properties of Lb. plantarum and its effect on rats in which carcinogenesis has been chemically initiated [ 50 ]. In the feces of animals treated with the probiotic, an increased number of Lactobacillus spp.…”

Association between Probiotics and Modulation of Gut Microbial Community Composition in Colorectal Cancer Animal Models: A Systematic Review (2010–2021)

“…For instance, the genera Bifdobacterium and Lactobacillus displayed minimal β-glucuronidase activity for modifying the microbiota [65]. In addition, based on two studies [41,50] in our review, β-glucuronidase activity decreased in response to probiotic and synbiotic supplementation. While Zhu et al [34] identifed β-glucosidase and β-glucuronidase showed no alterations in L. salivarius-treated cancerous animals, still azoreductase activity was reduced in this group, indicating a possible protective efect against carcinogenesis by decreasing levels of carcinogen activation and DNA mutation.…”

“…Among 33 articles included, the most prominent cancer agent was azoxymethane/dextran sodium sulfate (AOM/DSS) (14 studies) [ 23 , 25 – 29 , 33 , 35 , 36 , 39 , 40 , 45 , 46 , 48 ]. The second most commonly used cancer agent was 1,2-dimethylhydrazine (DMH) as the inducing agent of preneoplastic lesions and tumors were induced, and the doses varied from 10 to 40 mg/kg (11 studies) [ 21 , 22 , 32 , 34 , 37 , 41 – 43 , 49 – 51 ]. In 3 studies, genetically modified animals, in which disease developed spontaneously, were used [ 20 , 24 , 31 ].…”

“…Fecal enzymes, i.e., β -glucuronidase, azoreductase, and β -glucosidase have been implicated in converting procarcinogens into carcinogens; thus, the activity of these enzymes was assessed to deduce the modulating potential of probiotics in the colonic environment. Only three studies [ 34 , 41 , 50 ] reported changes in fecal enzyme activity in probiotic and synbiotic-treated groups ( Table 2 ). In 2 studies, fecal enzymes were assessed along with bacterial microbiota compositions and SCFAs [ 34 , 50 ].…”

“…For instance, the genera Bifidobacterium and Lactobacillus displayed minimal β -glucuronidase activity for modifying the microbiota [ 65 ]. In addition, based on two studies [ 41 , 50 ] in our review, β -glucuronidase activity decreased in response to probiotic and synbiotic supplementation. While Zhu et al [ 34 ] identified β -glucosidase and β -glucuronidase showed no alterations in L .…”