Effect of cultured white soft cheese on the histopathological changes in the kidneys and liver of albino rats

Abstract

Three different types of lactic acid bacteria (Lactobacillus helveticus, Lactobacillus rhamnosus and Streptococcus thermophilus S3855) were used to manufacture white soft cheese. The resultant white soft cheeses were pickled for 28 days at refrigerator temperatures and were fed to the experimental rats. The chemical and microbiological analyses of white soft cheese were conducted at different storage periods (fresh, 7 days, 14 days, 21 days, and 28 days). The pH values and protein content of white soft cheese gradually decreased during the storage peroid. Conversely, the moisture content, titratable acidity, and fat/DM % of white soft cheese were found to increase with of the increase in pickling periods of up to 28 days. Microbiologically, the total viable count of bacteria in the control samples was lower than that in the other treatments. Furthermore, the treatments containing the L. helveticus and L. rhamnosus strains had the highest lactoacilli counts whereas the treatment containing the S. thermophilus strain had the highest streptococci counts. Twenty-five male Albino rats were used for experiemntal technique. Rats were fed with 70% basal diet with addition of 30% white soft cheese. Several pathological findings were present in all experimental groups apart from the control rats, and the kidney samples exhibited renal vascular congestion especially in the cortical area. The changes of the glomeruli comprise atrophy, distortion, hypocellularity of the glomerular tuft, and focal lymphoid cell reactions. The renal tubular epithelium showed a series of degenerative changes ranging up to necrosis. The liver samples showed variable hepatic injury in the form of thickening in the Glisson capsule, as well as dissociation and disorganization of hepatic cords. Hepatocellular vacuolar degeneration, presence of focal areas of nodular hyperplasia, the hyperplastic cells mixed with lymphocytic infiltration, congestion in the portal vein, periportal fibrosis and edema with the presence of newly formed nonfunctional bile ductulus. Based on the histopathology scores, the severity of renal and hepatic changes was significantly increased (P ≤ 0.05) in all of the experimental groups compared with the control group. Generally, the chemical composition, microbiological analysis and vital organs were significantly affected by using cultured white soft cheese.

Wheat Germ Oil and Propolis Decrease Parasite Burden and Restore Marked Histopathological Changes in Liver and Lung in Mice with Chronic Toxoplasmosis

“…Data (mean ± standard deviation) were compared between groups using a t -test performed with the Statistical Program for Social Science version 2. Histopathological scoring data were compared using one-way analysis of variance (ANOVA) with Tukey’s post hoc multiple comparison test with GraphPad Prism, version 5 (San Diego, California, CA, USA) [ 53 ]. A p -value < 0.05 was considered to indicate a statistically significant difference.…”

Melatonin ameliorates serobiochemical alterations and restores the cardio-nephro diabetic vascular and cellular alterations in streptozotocin-induced diabetic rats

“…The histopathological measurements from the experimental groups were stated as mean ± standard deviation (SD) and estimated using Version 5 of GraphPad Prism (San Diego, California, USA) for analyzing data using the one-way ANOVA and Tukey's post hoc multiple-comparisons tests; the statistical significance was considered at a P-value of <0.05 (52,53). As regards the serum biochemical parameters, data were collected and then analyzed using SPSS.28 software.…”

Effect of tuft-IL-25-ILC2 pathway on mouse small intestine stimulated byTrichinella spiralisES antigen

“…Doublecortin-like kinase 1 protein (DCLK1) marks differentiated tuft cells in the small intestine and colon epithelium [11]. Cluster cell-derived IL-25 helps drive the feed-forward Tuft-ILC2 signaling pathway [12], in which ILC2 is activated to produce IL-5 and IL-13, thereby promoting type 2 inflammation.…”

“…Consistent with the above results, it was found that IL-25 might affect the expression of related factors in the Tuft-IL-25-ILC2 pathway in the ES antigen-stimulated mouse small intestine. Doublecortin-like kinase 1 protein (DCLK1) marks differentiated tuft cells in the small intestine and colon epithelium [11]. Cluster cell-derived IL-25 helps drive the feed-forward Tuft-ILC2 signaling pathway [12], in which ILC2 is activated to produce IL-5 and IL-13, thereby promoting type 2 inflammation.…”