The effect of probiotic bacteria on the adhesion of pathogens to human intestinal mucus

Tuomola, Elina; Ouwehand, Arthur C.; Salminen, Seppo

doi:10.1111/j.1574-695x.1999.tb01381.x

Cited by 186 publications

(94 citation statements)

References 30 publications

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“…It was observed for example that there was a dramatic decrease in the adhesive ability of Lb. rhamnosus GG whatever the intestinal matrix model (Tuomola et al, 1999) or on Caco-2 cells (Ouwehand et al, 2000). The reduction of adhesion can be explained by the heatsensitive proteinaceous nature of the molecules involved in Lb.…”

Section: Discussionmentioning

confidence: 99%

“…This enhances the intestinal persistence of the probiotic bacteria, and limits pathogen access to the epithelium (O'Hara & Shanahan, 2007). In general, the incubation of enteropathogens with probiotic strains having a high coaggregation potential decreases the adhesion of the pathogen to the intestinal mucus (Tuomola et al, 1999), as well as the invasive capacity of pathogens on enterocytes (Golowczyc et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

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In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

112

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Bacterial aggregation and/or adhesion are key factors for colonization of the digestive ecosystem and the ability of probiotic strains to exclude pathogens. In the present study, two probiotic strains, Lactobacillus rhamnosus CNCM-I-3698 and Lactobacillus farciminis CNCM-I-3699, were evaluated as viable or heat-killed forms and compared with probiotic reference Lactobacillus strains (Lb. rhamnosus GG and Lb. farciminis CIP 103136). The autoaggregation potential of both forms was higher than that of reference strains and twice that of pathogenic strains. The coaggregation potential of these two beneficial micro-organisms was evaluated against several pathogenic agents that threaten the global safety of the feed/food chain: Escherichia coli, Salmonella spp., Campylobacter spp. and Listeria monocytogenes. The strongest coaggregative interactions were demonstrated with Campylobacter spp. by a coaggregation test, confirmed by electron microscopic examination for the two forms. Viable forms were investigated for the nature of the bacterial cell-surface molecules involved, by sugar reversal tests and chemical and enzymic pretreatments. The results suggest that the coaggregation between both probiotic strains and C. jejuni CIP 70.2 T is mediated by a carbohydrate-lectin interaction. The autoaggregation potential of the two probiotics decreased upon exposure to proteinase, SDS or LiCl, showing that proteinaceous components on the surface of the two lactobacilli play an important role in this interaction. Adhesion abilities of both Lactobacillus strains were also demonstrated at significant levels on Caco-2 cells, mucin and extracellular matrix material. Both viable and heat-killed forms of the two probiotic lactobacilli inhibited the attachment of C. jejuni CIP 70.2 T to mucin. In conclusion, in vitro assays showed that Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, as viable or heat-killed forms, are adherent to different intestinal matrix models and are highly aggregative in vitro with pathogens, especially Campylobacter spp., the most commonly reported zoonotic agent in the European Union. This study supports the need for further in vivo investigations to demonstrate the potential food safety benefits of Lb. rhamnosus CNCM-I-3698 and Lb. farciminis CNCM-I-3699, live or heat-killed, in the global feed/food chain.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

Tareb

Bernardeau²,

Guéguen

et al. 2013

Journal of Medical Microbiology

112

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“…It has been previously reported that the probiotic strains L. johnsonii La1 (2,18,27), L. rhamnosus GG (11-14, 16, 26, 27), L. casei Shirota YT9029 (12,13,20,25,27), and L. rhamnosus GR1 (4, 17, 22) exert antagonistic activity against gram-negative pathogens. We observed that the La1, GG, YT9029, GR1, and DN-114001 strains acted mainly by secreting a non-lactic acid molecule(s) into the CFCS.…”

Section: Discussionmentioning

confidence: 99%

pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium

Fayol-Messaoudi

Berger

Coconnier-Polter

et al. 2005

Appl Environ Microbiol

244

163

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The mechanism(s) underlying the antibacterial activity of probiotic Lactobacillus strains appears to be multifactorial and includes lowering of the pH and the production of lactic acid and of antibacterial compounds, including bacteriocins and nonbacteriocin, non-lactic acid molecules. Addition of Dulbecco's modified Eagle's minimum essential medium to the incubating medium delays the killing activity of lactic acid. We found that the probiotic strains Lactobacillus johnsonii La1, Lactobacillus rhamnosus GG, Lactobacillus casei Shirota YIT9029, L. casei DN-114 001, and L. rhamnosus GR1 induced a dramatic decrease in the viability of Salmonella enterica serovar Typhimurium SL1344 mainly attributable to non-lactic acid molecule(s) present in the cell-free culture supernatant (CFCS). These molecules were more active against serovar Typhimurium SL1344 in the exponential growth phase than in the stationary growth phase. We also showed that the production of the non-lactic acid substance(s) responsible for the killing activity was dependent on growth temperature and that both unstable and stable substances with killing activity were present in the CFCSs. We found that the complete inhibition of serovar Typhimurium SL1344 growth results from a pH-lowering effect.

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“…Several probiotics have been demonstrated to enhance gastrointestinal health by stimulation of host immunity and inhibition of pathogen adherence to mucus and epithelial cells (reviewed in references 7 and 44). The ability of lactobacilli to attach to epithelial cells (4,5,26,36,51) and mucins (27,34,43,(48)(49)(50) has been documented and is expected to be an important characteristic, enhancing intestinal persistence and antagonistic competition with pathogens, especially at the point of initial contact with the mucosa. Recently, the association of Lactobacillus species with Peyer's patches in mice has been described (40).…”

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confidence: 99%

GroEL ofLactobacillus johnsoniiLa1 (NCC 533) Is Cell Surface Associated: Potential Role in Interactions with the Host and the Gastric PathogenHelicobacter pylori

et al. 2006

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Heat shock proteins of the GroEL or Hsp60 class are highly conserved proteins essential to all living organisms. Even though GroEL proteins are classically considered intracellular proteins, they have been found at the surface of several mucosal pathogens and have been implicated in cell attachment and immune modulation. The purpose of the present study was to investigate the GroEL protein of a gram-positive probiotic bacterium, Lactobacillus johnsonii La1 (NCC 533). Its presence at the bacterial surface was demonstrated using a whole-cell enzyme-linked immunosorbent assay and could be detected in bacterial spent culture medium by immunoblotting. To assess binding of La1 GroEL to mucins and intestinal epithelial cells, the La1 GroEL protein was expressed in Escherichia coli. We report here that La1 recombinant GroEL (rGroEL) binds to mucins and epithelial cells and that this binding is pH dependent. Immunomodulation studies showed that La1 rGroEL stimulates interleukin-8 secretion in macrophages and HT29 cells in a CD14-dependent mechanism. This property is common to rGroEL from other gram-positive bacteria but not to the rGroEL of the gastric pathogen Helicobacter pylori. In addition, La1 rGroEL mediates the aggregation of H. pylori but not that of other intestinal pathogens. Our in vitro results suggest that GroEL proteins from La1 and other lactic acid bacteria might play a role in gastrointestinal homeostasis due to their ability to bind to components of the gastrointestinal mucosa and to aggregate H. pylori.

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The effect of probiotic bacteria on the adhesion of pathogens to human intestinal mucus

Cited by 186 publications

References 30 publications

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

In vitro characterization of aggregation and adhesion properties of viable and heat-killed forms of two probiotic Lactobacillus strains and interaction with foodborne zoonotic bacteria, especially Campylobacter jejuni

pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium

GroEL ofLactobacillus johnsoniiLa1 (NCC 533) Is Cell Surface Associated: Potential Role in Interactions with the Host and the Gastric PathogenHelicobacter pylori

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