pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against
            <i>Salmonella enterica</i>
            Serovar Typhimurium

Fayol-Messaoudi, Domitille; Berger, Cédric N.; Coconnier-Polter, Marie-Hélène; Moal, Vanessa Liévin-Le; Servin, Alain L.

doi:10.1128/aem.71.10.6008-6013.2005

Cited by 235 publications

(180 citation statements)

References 27 publications

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“…However, there have been numerous reports of lactobacilli and bifidobacteria inhibiting the growth of Gramnegative bacteria by a mechanism(s) that do not involve pH reduction or volatile fatty acid production (e.g. CoconnierPolter et al, 2005;Fayol-Messaoudi et al, 2005).…”

Section: Action Of Probiotics On Other Bacteriamentioning

confidence: 99%

Probiotics – do they have a role in the pig industry?

Kenny

Smidt

Mengheri

et al. 2011

Animal

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The delivery of certain living microorganisms in food has long been suggested as having positive health effects in humans. This practice has extended into food animal production, with a variety of microorganisms being used; lactic acid bacteria, variousBacillusspecies and the yeastSaccharomyces cerevisiaehave been particularly used in the pig industry. The increased interest in probiotics is essentially due to the problem of microbial resistance to antibiotics and following the ban of the use of antibiotics in animal production, probiotics being considered an alternative means to reduce pathogen infection and improve animal health especially around the time of weaning. However, there is still a need to clarify the probiotic effectiveness in pigs, and the underlying mechanisms. When assessing the efficacy of probiotics one must consider the particular strain of organism being used and the production stage of the pigs being treated. The reproducible delivery of probiotics in industrial pig production is problematic as maintenance of viability is key to their beneficial activity, but difficult to achieve with commonly used feed processing technologies. One specific context where probiotics organisms may be reliably delivered is in systems utilising fermented liquid feeds. Liquid feed may be fermented by the activity of wild lactic acid bacteria or may be stimulated using specific isolates as ‘starters’; the latter system has advantages in terms of reproducibility and speed of fermentation. The farm context in which the organism is used is likely to be critical; the use of probiotics is more likely to result in measurable economic gains in animals living in sub-optimal conditions rather than in those reared in the highest welfare and husbandry conditions. The establishment of a beneficial lactic acid bacteria population at birth may lead to healthier animals, this may be most effectively achieved by treating sows, which provide an amplification step and flood the neonatal pigs’ environment with desirable bacterial strains. In contrast, it may be sufficient to provide a supportive, protective microbiota around the time of weaning as this is a time of major crisis with instability and loss of certain bacterial populations.

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Section: Action Of Probiotics On Other Bacteriamentioning

confidence: 99%

Probiotics – do they have a role in the pig industry?

Kenny

Smidt

Mengheri

et al. 2011

Animal

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“…Because the bacterial cell wall is comprised mainly of phospholipids, bile salt which is an emulsifier and solubilizes the lipid that can damage the bacterial cells [21]. [23,24]. Probiotic bacteria can lower the luminal pH through secretion of acetic and lactic acids, which inhibits the growth of some pathogens, including enterohemorrhagic E. coli (EHEC) [25].…”

Section: Lactid Acid Bacteria Survival Test On Acidity and Bile Saltsmentioning

confidence: 99%

Identification and Characterization Indigenous of Lactobacillus spfrom Bovine Rumen Fluidof Slaughterhouse

Nurhajati

Soepranianondo

Lokapirnasari

et al. 2017

KLS

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The discovery and characterisation of indigenous lactic acid bacteria (LAB) are important for diversity microbes as candidate probiotic. This research was aimed to identify lactic acid bacteria isolate from isolation process of local bovine rumen fluid from slaughterhouse in Surabaya Indonesia. Genotypic testing was conducted by analyzing 16S rDNA and biochemical identification. DNA of sample isolate was isolated and then amplified in vitro through the PCR method. Determination of nucleotide sequence of 16S rDNA was performed with sequencing method. The result of nucleotide sequence was than compared with GenBank database. The BLAST was then applied to identify the phylogenetic tree. Based on the biochemical characterization and nucleotide sequences, that isolate was identified as Lactobacillus rhamnosus subsp TG15. The result of this research showed that L.rhamnosus subsp TG15 showed viability bacteria in MRSA as control as much as 1.1 × 10 8 CFU/ml, mean while in MRSA pH 2, L.rhamnosus subsp TG15 showed its viability as much as 9.3 × 10 6 CFU/ml. Viability of isolate on bile tolerance 0.3% was 2.4 × 10 7 CFU/ml). Index antagonist bacteria test on S.aureus showed inhibition diameter as much as 2.0 mm and in antagonist test on E.coli as much as 2.5 mm. Based on the result, it could be concluded that this research found a new strain of lactic acid bacteria, L.rhamnosus subsp TG15 and that isolate has ability as the probiotic candidate.

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“…They may beneficially affect the host upon ingestion by a variety of potential or proven mechanisms (13,41). Similarly to bifidobacteria, lactobacilli are normal inhabitants of human and animal intestines.…”

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confidence: 99%

Characterization of Endogenous Plasmids from Lactobacillus salivarius UCC118

Fang

Flynn²,

et al. 2008

Appl Environ Microbiol

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The genome of Lactobacillus salivarius UCC118 comprises a 1.83-Mb chromosome, a 242-kb megaplasmid (pMP118), and two smaller plasmids of 20 kb (pSF118-20) and 44 kb (pSF118-44). Annotation and bioinformatic analyses suggest that both of the smaller plasmids replicate by a theta replication mechanism. Furthermore, it appears that they are transmissible, although neither possesses a complete set of conjugation genes. Plasmid pSF118-20 encodes a toxin-antitoxin system composed of pemI and pemK homologs, and this plasmid could be cured when PemI was produced in trans. The minimal replicon of pSF118-20 was determined by deletion analysis. Shuttle vector derivatives of pSF118-20 were generated that included the replication region (pLS203) and the replication region plus mobilization genes (pLS208). The plasmid pLS203 was stably maintained without selection in Lactobacillus plantarum, Lactobacillus fermentum, and the pSF118-20-cured derivative strain of L. salivarius UCC118 (strain LS201). Cloning in pLS203 of genes encoding luciferase and green fluorescent protein, and expression from a constitutive L. salivarius promoter, demonstrated the utility of this vector for the expression of heterologous genes in Lactobacillus. This study thus expands the knowledge base and vector repertoire of probiotic lactobacilli.Lactic acid bacteria and in particular members of the genus Lactobacillus are the most common microbes used as probiotics. They may beneficially affect the host upon ingestion by a variety of potential or proven mechanisms (13,41). Similarly to bifidobacteria, lactobacilli are normal inhabitants of human and animal intestines. Among the more than 100 species of the genus Lactobacillus that have been identified, those that are used as . rhamnosus, and L. salivarius (32). Genome sequence availability considerably facilitates the identification of probiotic characteristics of these bacteria and the prediction of their behaviors in the human gastrointestinal (GI) tract. To date, 11 Lactobacillus genome sequences have been published, and at least 11 additional sequencing projects are in progress (6). This information has dramatically improved our understanding of the metabolic processes and genetics of these microorganisms, as well as their potential roles in health promotion of their hosts. However, for the targeted analysis of genes that contribute to probiotic characteristics, the development of molecular tools for these lactobacilli is of paramount importance.Plasmids, autonomously replicating extrachromosomal genetic elements, are widely present in the genus Lactobacillus. About 38% of the species in this genus contain plasmids (60). Endogenous plasmids from Lactobacillus are of interest because of the traits they confer upon the host. For example, these plasmids may harbor genes encoding resistance to antibiotics (39, 54) and metal ions (55), genes encoding bacteriocins (30, 44), gene clusters for conjugation (55), genes involved in adherence and biotin metabolism (10), and genes encoding toxin-antitoxin (TA) pr...

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pH-, Lactic Acid-, and Non-Lactic Acid-Dependent Activities of Probiotic Lactobacilli against Salmonella enterica Serovar Typhimurium

Cited by 235 publications

References 27 publications

Probiotics – do they have a role in the pig industry?

Probiotics – do they have a role in the pig industry?

Identification and Characterization Indigenous of Lactobacillus spfrom Bovine Rumen Fluidof Slaughterhouse

Characterization of Endogenous Plasmids from Lactobacillus salivarius UCC118

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