The effect of Pluronic F-68 on hybridoma cells in continuous culture

Al‐Rubeai, Mohamed; Emery, A. N.; Chalder, S.

doi:10.1007/bf00174201

Cited by 22 publications

(9 citation statements)

References 7 publications

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“…Unfortunately, even low-level turbulence is detrimental to dinoflagellates [2, 6-9, 11, 24]. One approach to improving survival of dinoflagellates in a relatively turbulent environment may be to use protective additives such as carboxymethyl cellulose (CMC) or Pluronic F68 (PF68) that have been shown to reduce or prevent shear-associated damage to fragile animal cells [15][16][17] and certain nondinoflagellate shear-sensitive microalgae [18,19]. This was the rationale for assessing the usefulness of CMC and PF68 in culturing P. reticulatum.…”

Section: Resultsmentioning

confidence: 99%

“…Changes in the fluidity of the cell membrane are another factor that is known to affect the susceptibility of certain cells to hydrodynamic forces [15,25]. PF68 reduces fluidity of the membrane of animal cells [25] to make them more robust and may have a similar action on dinoflagellates.…”

Section: Relative Cell Viability Was Low Compared To Cells Inmentioning

confidence: 99%

“…Shear protective additives such as carboxymethyl cellulose (CMC) and Pluronic F68 have been used previously to enhance survival of fragile cells [15][16][17] including some microalgae [18,19] in conditions that would be otherwise damaging. Similar methods have been found useful for culturing dinoflagellates [11,13,20], although few studies exist.…”

Section: Introductionmentioning

confidence: 99%

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Carboxymethyl cellulose and Pluronic F68 protect the dinoflagellate Protoceratium reticulatum against shear-associated damage

Rodríguez

Sánchez-Mirón

Camacho

et al. 2010

Bioprocess Biosyst Eng

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The red-tide dinoflagellate Protoceratium reticulatum is shown to be protected against turbulence-associated damage by the use of the additives Pluronic F68 (PF68) and carboxymethyl cellulose (CMC) in the culture medium. Relative to agitated controls, these additives had a dose-dependent protective effect at concentrations of up to 0.4 and 0.5 g L(-1) for CMC and F68, respectively. In static cultures, these additives inhibited growth directly or indirectly at a concentration of >0.5 g L(-1). Compared to CMC, PF68 was a better protectant overall. Cell-specific production of yessotoxins was enhanced under elevated shear stress regimens so long as the turbulence intensity was insufficient to damage the cells outright. Shear-induced production of reactive oxygen species and direct effects of turbulence on the cell cycle contributed to the observed shear effects.

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Section: Resultsmentioning

confidence: 99%

Section: Relative Cell Viability Was Low Compared To Cells Inmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Carboxymethyl cellulose and Pluronic F68 protect the dinoflagellate Protoceratium reticulatum against shear-associated damage

Rodríguez

Sánchez-Mirón

Camacho

et al. 2010

Bioprocess Biosyst Eng

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“…However, there are offsetting disadvantages of using Pluronic F-68. These include the difficulties of removing Pluronic F-68 during product recovery as well as the potential inhibition of growth for some cell lines [42]. For these reasons, it may be important to minimize the concentration of Pluronic F-68 in the culture.…”

Section: Discussionmentioning

confidence: 99%

Pluronic Enhances the Robustness and Reduces the Cell Attachment of Mammalian Cells

et al. 2008

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The addition of the non-ionic surfactant, Pluronic F-68, to serum-free CHO cultures causes multi-functional effects that enhance cell yield in agitated cultures and reduce cell adhesion in stationary cultures. Three independent CHO cell lines were subjected to high liquid shear in assay systems that either included or excluded a liquid-gas interface. In the absence of Pluronic, there was a loss in cell viability in either assay system, although there was an intrinsic variability in sensitivity of the cell lines to shear damage. Supplementation with Pluronic prevented loss of cell viability, indicating protection in either a gas sparged or bubble-free environment. However, we found no evidence of long-term protection of cells once Pluronic was removed. Pluronic was capable of repairing trypsin-damaged cells as evidenced by enhanced growth, reduced membrane porosity, and improved robustness under liquid shear. The proportion of adherent cells was reduced to a minimal level by the presence of Pluronic although its effect was rapidly reversible with a high proportion (70%) of adherent cells observed within a few culture passages of its removal. The observed effects of Pluronic on these cultures are compatible with a mechanism in which the polymer forms a protective layer on the cell membrane, which has a significantly lower hydrophobicity.

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“…According to the results obtained, the agitation played an important role during the culture of suspension adapted 293 cells having a negative effect upon cell density and viability. The addition of a surfactant could help to overcome this problem, which has been reported previously for different mammalian cells [31][32][33][34][35].…”

Section: Discussionmentioning

confidence: 96%

Production of Adenoviral Vectors in 293 Cells: A Case Study of the Adaptation of Attached Cells to Grow in Suspension

Negrete¹,

Ling²,

Lyddiatt³

2008

TOBIOTJ

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A study of the production of adenoviral vectors in suspension 293 cells has been explored. A defined serumfree medium (293 SFM II) formulated without human or animal origin components from Invitrogen was used for the suspension adapted 293 cells. It was demonstrated that the 293 cells can be adapted to grow in suspension using serum free medium. The effect of different cell culture parameters was determined. The production technique demonstrated here is expected to simplify purification processes and circumvents the problems associated with serum containing medium.

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The effect of Pluronic F-68 on hybridoma cells in continuous culture

Cited by 22 publications

References 7 publications

Carboxymethyl cellulose and Pluronic F68 protect the dinoflagellate Protoceratium reticulatum against shear-associated damage

Carboxymethyl cellulose and Pluronic F68 protect the dinoflagellate Protoceratium reticulatum against shear-associated damage

Pluronic Enhances the Robustness and Reduces the Cell Attachment of Mammalian Cells

Production of Adenoviral Vectors in 293 Cells: A Case Study of the Adaptation of Attached Cells to Grow in Suspension

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